Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Thais Almeida Marques da
Data de Publicação: 2015
Outros Autores: Gomes, Luciana Inácia, Oliveira, Edward, Vital, Wendel Coura, Silva, Letícia de Azevedo, Pais, Fabiano Sviatopolk Mirsky, Ker, Henrique Gama, Reis, Alexandre Barbosa, Rabello, Ana Lucia Teles, Carneiro, Mariangela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12666
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
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spelling Silva, Thais Almeida Marques daGomes, Luciana InáciaOliveira, EdwardVital, Wendel CouraSilva, Letícia de AzevedoPais, Fabiano Sviatopolk MirskyKer, Henrique GamaReis, Alexandre BarbosaRabello, Ana Lucia TelesCarneiro, Mariangela2016-02-01T11:46:00Z2016-02-01T11:46:00Z2015SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/1266610.1186/s13071-015-0837-y.engBioMed CentralGenetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Toxoplasmose Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil.BACKGROUND: Certain municipalities in the Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil, have the highest human visceral leishmaniasis (VL) mortality rates in the country and also demonstrate high canine seropositivity. In Brazil, the etiologic agent of VL is Leishmania (Leishmania) infantum. The aim of this study was to evaluate the intraspecific genetic variability of parasites from humans and from dogs with different clinical forms of VL in five municipalities of BHMA using PCR-RFLP and two target genes: kinetoplast DNA (kDNA) and gp63. METHODS: In total, 45 samples of DNA extracted from clinical samples (n = 35) or L. infantum culture (n = 10) were evaluated. These samples originated from three groups: adults (with or without Leishmania/HIV co-infection; n = 14), children (n = 18) and dogs (n = 13). The samples were amplified for the kDNA target using the MC1 and MC2 primers (447 bp), while the Sg1 and Sg2 (1330 bp) primers were used for the gp63 glycoprotein target gene. RESULTS: The restriction enzyme patterns of all the samples tested were monomorphic. CONCLUSIONS: These findings reveal a high degree of genetic homogeneity for the evaluated gene targets among L. infantum samples isolated from different hosts and representing different clinical forms of VL in the municipalities of BHMA studied.Leishmania (Leishmania) infantumIntraspecific genetic variabilityPCR-RFLPinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12666/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdfGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdfapplication/pdf10721186https://www.arca.fiocruz.br/bitstream/icict/12666/2/Genetic%20homogeneity%20among%20Leishmania%20%28Leishmania%29%20infantum%20isolates.pdf6344edd6cdea306724b13c911ba8a110MD52TEXTGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdf.txtGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdf.txtExtracted texttext/plain696https://www.arca.fiocruz.br/bitstream/icict/12666/3/Genetic%20homogeneity%20among%20Leishmania%20%28Leishmania%29%20infantum%20isolates.pdf.txtc11d0ef9c17ba436738803447e98c364MD53icict/126662019-06-19 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dc.title.pt_BR.fl_str_mv Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
title Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
spellingShingle Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
Silva, Thais Almeida Marques da
Leishmania (Leishmania) infantum
Intraspecific genetic variability
PCR-RFLP
title_short Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
title_full Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
title_fullStr Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
title_full_unstemmed Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
title_sort Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
author Silva, Thais Almeida Marques da
author_facet Silva, Thais Almeida Marques da
Gomes, Luciana Inácia
Oliveira, Edward
Vital, Wendel Coura
Silva, Letícia de Azevedo
Pais, Fabiano Sviatopolk Mirsky
Ker, Henrique Gama
Reis, Alexandre Barbosa
Rabello, Ana Lucia Teles
Carneiro, Mariangela
author_role author
author2 Gomes, Luciana Inácia
Oliveira, Edward
Vital, Wendel Coura
Silva, Letícia de Azevedo
Pais, Fabiano Sviatopolk Mirsky
Ker, Henrique Gama
Reis, Alexandre Barbosa
Rabello, Ana Lucia Teles
Carneiro, Mariangela
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Thais Almeida Marques da
Gomes, Luciana Inácia
Oliveira, Edward
Vital, Wendel Coura
Silva, Letícia de Azevedo
Pais, Fabiano Sviatopolk Mirsky
Ker, Henrique Gama
Reis, Alexandre Barbosa
Rabello, Ana Lucia Teles
Carneiro, Mariangela
dc.subject.en.pt_BR.fl_str_mv Leishmania (Leishmania) infantum
Intraspecific genetic variability
PCR-RFLP
topic Leishmania (Leishmania) infantum
Intraspecific genetic variability
PCR-RFLP
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-02-01T11:46:00Z
dc.date.available.fl_str_mv 2016-02-01T11:46:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12666
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-0837-y.
identifier_str_mv SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015.
1756-3305
10.1186/s13071-015-0837-y.
url https://www.arca.fiocruz.br/handle/icict/12666
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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