Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12666 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. |
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Silva, Thais Almeida Marques daGomes, Luciana InáciaOliveira, EdwardVital, Wendel CouraSilva, Letícia de AzevedoPais, Fabiano Sviatopolk MirskyKer, Henrique GamaReis, Alexandre BarbosaRabello, Ana Lucia TelesCarneiro, Mariangela2016-02-01T11:46:00Z2016-02-01T11:46:00Z2015SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/1266610.1186/s13071-015-0837-y.engBioMed CentralGenetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Toxoplasmose Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil.BACKGROUND: Certain municipalities in the Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil, have the highest human visceral leishmaniasis (VL) mortality rates in the country and also demonstrate high canine seropositivity. In Brazil, the etiologic agent of VL is Leishmania (Leishmania) infantum. The aim of this study was to evaluate the intraspecific genetic variability of parasites from humans and from dogs with different clinical forms of VL in five municipalities of BHMA using PCR-RFLP and two target genes: kinetoplast DNA (kDNA) and gp63. METHODS: In total, 45 samples of DNA extracted from clinical samples (n = 35) or L. infantum culture (n = 10) were evaluated. These samples originated from three groups: adults (with or without Leishmania/HIV co-infection; n = 14), children (n = 18) and dogs (n = 13). The samples were amplified for the kDNA target using the MC1 and MC2 primers (447 bp), while the Sg1 and Sg2 (1330 bp) primers were used for the gp63 glycoprotein target gene. RESULTS: The restriction enzyme patterns of all the samples tested were monomorphic. CONCLUSIONS: These findings reveal a high degree of genetic homogeneity for the evaluated gene targets among L. infantum samples isolated from different hosts and representing different clinical forms of VL in the municipalities of BHMA studied.Leishmania (Leishmania) infantumIntraspecific genetic variabilityPCR-RFLPinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12666/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdfGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdfapplication/pdf10721186https://www.arca.fiocruz.br/bitstream/icict/12666/2/Genetic%20homogeneity%20among%20Leishmania%20%28Leishmania%29%20infantum%20isolates.pdf6344edd6cdea306724b13c911ba8a110MD52TEXTGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdf.txtGenetic homogeneity among Leishmania (Leishmania) infantum isolates.pdf.txtExtracted texttext/plain696https://www.arca.fiocruz.br/bitstream/icict/12666/3/Genetic%20homogeneity%20among%20Leishmania%20%28Leishmania%29%20infantum%20isolates.pdf.txtc11d0ef9c17ba436738803447e98c364MD53icict/126662019-06-19 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dc.title.pt_BR.fl_str_mv |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
title |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
spellingShingle |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil Silva, Thais Almeida Marques da Leishmania (Leishmania) infantum Intraspecific genetic variability PCR-RFLP |
title_short |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
title_full |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
title_fullStr |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
title_full_unstemmed |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
title_sort |
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil |
author |
Silva, Thais Almeida Marques da |
author_facet |
Silva, Thais Almeida Marques da Gomes, Luciana Inácia Oliveira, Edward Vital, Wendel Coura Silva, Letícia de Azevedo Pais, Fabiano Sviatopolk Mirsky Ker, Henrique Gama Reis, Alexandre Barbosa Rabello, Ana Lucia Teles Carneiro, Mariangela |
author_role |
author |
author2 |
Gomes, Luciana Inácia Oliveira, Edward Vital, Wendel Coura Silva, Letícia de Azevedo Pais, Fabiano Sviatopolk Mirsky Ker, Henrique Gama Reis, Alexandre Barbosa Rabello, Ana Lucia Teles Carneiro, Mariangela |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Thais Almeida Marques da Gomes, Luciana Inácia Oliveira, Edward Vital, Wendel Coura Silva, Letícia de Azevedo Pais, Fabiano Sviatopolk Mirsky Ker, Henrique Gama Reis, Alexandre Barbosa Rabello, Ana Lucia Teles Carneiro, Mariangela |
dc.subject.en.pt_BR.fl_str_mv |
Leishmania (Leishmania) infantum Intraspecific genetic variability PCR-RFLP |
topic |
Leishmania (Leishmania) infantum Intraspecific genetic variability PCR-RFLP |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-02-01T11:46:00Z |
dc.date.available.fl_str_mv |
2016-02-01T11:46:00Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/12666 |
dc.identifier.issn.none.fl_str_mv |
1756-3305 |
dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-015-0837-y. |
identifier_str_mv |
SILVA, Thais Almeida Marques da et al. Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA); Minas Gerais; Brazil. Parasit Vectors., vol. 8, p. 226, 2015. 1756-3305 10.1186/s13071-015-0837-y. |
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https://www.arca.fiocruz.br/handle/icict/12666 |
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eng |
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eng |
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BioMed Central |
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BioMed Central |
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