Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117921 |
Resumo: | Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. |
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Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles.MutationsGeneMycobacterium bovis BCGGenomeVirulenceMycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.Mercedes Bigi, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Cristina Lourdes Vazquez, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Ana Beatriz C Castelão, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Elizabeth Andrea García, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Angel A Cataldi, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Mary Jackson, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Michael McNeil, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Marcelo Soria, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Martín J Zumárraga, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Matias Cabruja, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Gabriela Gago, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Federico C Blanco, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Christiane Nishibef, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; Nalvo F Almeida, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; FLABIO RIBEIRO DE ARAUJO, CNPGC; Fabiana Bigi, Instituto de Biotecnología, IABIMO, CICVyA/INTA.BIGI, M.VAZQUEZ, C. L.CASTELÃO, A. B. C.GARCÍA, E. A.CATALDI, A. A.JACKSON, M.MAcNEIL, M.SORIA, M.ZUMÁRRAGA, M. J.MATIAS, C.GAGO, G.BLANCO, F. C.NISHIBEF, C.ALMEIDA, N. F.ARAUJO, F. R.BIGI, F.2019-12-31T00:37:05Z2019-12-31T00:37:05Z2019-12-3020192019-12-31T00:37:05Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVeterinary Microbiology, v. 239, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117921enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-12-31T00:37:12Zoai:www.alice.cnptia.embrapa.br:doc/1117921Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-12-31T00:37:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-12-31T00:37:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
title |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
spellingShingle |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. BIGI, M. Mutations Gene Mycobacterium bovis BCG Genome Virulence |
title_short |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
title_full |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
title_fullStr |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
title_full_unstemmed |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
title_sort |
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles. |
author |
BIGI, M. |
author_facet |
BIGI, M. VAZQUEZ, C. L. CASTELÃO, A. B. C. GARCÍA, E. A. CATALDI, A. A. JACKSON, M. MAcNEIL, M. SORIA, M. ZUMÁRRAGA, M. J. MATIAS, C. GAGO, G. BLANCO, F. C. NISHIBEF, C. ALMEIDA, N. F. ARAUJO, F. R. BIGI, F. |
author_role |
author |
author2 |
VAZQUEZ, C. L. CASTELÃO, A. B. C. GARCÍA, E. A. CATALDI, A. A. JACKSON, M. MAcNEIL, M. SORIA, M. ZUMÁRRAGA, M. J. MATIAS, C. GAGO, G. BLANCO, F. C. NISHIBEF, C. ALMEIDA, N. F. ARAUJO, F. R. BIGI, F. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Mercedes Bigi, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Cristina Lourdes Vazquez, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Ana Beatriz C Castelão, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Elizabeth Andrea García, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Angel A Cataldi, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Mary Jackson, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Michael McNeil, Colorado State University, Dept. of Microbiology, Immunology and Pathology; Marcelo Soria, Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología Agrícola, INBA-CONICET; Martín J Zumárraga, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Matias Cabruja, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Gabriela Gago, Laboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Federico C Blanco, Instituto de Biotecnología, IABIMO, CICVyA/INTA; Christiane Nishibef, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; Nalvo F Almeida, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Computação; FLABIO RIBEIRO DE ARAUJO, CNPGC; Fabiana Bigi, Instituto de Biotecnología, IABIMO, CICVyA/INTA. |
dc.contributor.author.fl_str_mv |
BIGI, M. VAZQUEZ, C. L. CASTELÃO, A. B. C. GARCÍA, E. A. CATALDI, A. A. JACKSON, M. MAcNEIL, M. SORIA, M. ZUMÁRRAGA, M. J. MATIAS, C. GAGO, G. BLANCO, F. C. NISHIBEF, C. ALMEIDA, N. F. ARAUJO, F. R. BIGI, F. |
dc.subject.por.fl_str_mv |
Mutations Gene Mycobacterium bovis BCG Genome Virulence |
topic |
Mutations Gene Mycobacterium bovis BCG Genome Virulence |
description |
Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-31T00:37:05Z 2019-12-31T00:37:05Z 2019-12-30 2019 2019-12-31T00:37:05Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Veterinary Microbiology, v. 239, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117921 |
identifier_str_mv |
Veterinary Microbiology, v. 239, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117921 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503487139086336 |