A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice

Detalhes bibliográficos
Autor(a) principal: Carlétti, Dyego
Data de Publicação: 2013
Outros Autores: Fonseca, Denise Morais da, Gembre, Ana Flavia, Masson, Ana Paula, Campos, Lívia Weijenborg, Leite, Luciana C. C., Pires, Andréa Rodrigues, Vieira, Joseli Lannes, Silva, Célio Lopes, Bonato, Vânia Luiza Deperon, Horn, Cynthia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11676
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil.
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spelling Carlétti, DyegoFonseca, Denise Morais daGembre, Ana FlaviaMasson, Ana PaulaCampos, Lívia WeijenborgLeite, Luciana C. C.Pires, Andréa RodriguesVieira, Joseli LannesSilva, Célio LopesBonato, Vânia Luiza DeperonHorn, Cynthia2015-09-21T17:25:10Z2015-09-21T17:25:10Z2013CARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.https://www.arca.fiocruz.br/handle/icict/1167610.1128/CVI.00148-13engAmerican Society for MicrobiologyA Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Instituto Butantã. Centro de Biotecnologia. São Paulo, SP, brasil.Fonte Medicina Diagnóstica. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, BrasilMycobacterium bovis BCG prime DNA (Mycobacterium tuberculosis genes)-booster vaccinations have been shown to induce greater protection against tuberculosis (TB) than BCG alone. This heterologous prime-boost strategy is perhaps the most realistic vaccination for the future of TB infection control, especially in countries where TB is endemic. Moreover, a prime-boost regimen using biodegradable microspheres seems to be a promising immunization to stimulate a long-lasting immune response. The alanine proline antigen (Apa) is a highly immunogenic glycoprotein secreted by M. tuberculosis. This study investigated the immune protection of Apa DNA vaccine against intratracheal M. tuberculosis challenge in mice on the basis of a heterologous prime-boost regimen. BALB/c mice were subcutaneously primed with BCG and intramuscularly boosted with a single dose of plasmid carrying apa and 6,6=-trehalose dimycolate (TDM) adjuvant, coencapsulated in microspheres (BCG-APA), and were evaluated 30 and 70 days after challenge. This prime-boost strategy (BCG-APA) resulted in a significant reduction in the bacterial load in the lungs, thus leading to better preservation of the lung parenchyma, 70 days postinfection compared to BCG vaccinated mice. The profound effect of this heterologous prime-boost regimen in the experimental model supports its development as a feasible strategy for prevention of TB.TuberculosisDNA VaccineMycobacterium bovisMycobacterium bovis BCG prime DNATuberculoseMycobacterium bovisVacina BCGinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11676/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjoseli_vieira_etal_IOC_2013.pdfapplication/pdf2121105https://www.arca.fiocruz.br/bitstream/icict/11676/2/joseli_vieira_etal_IOC_2013.pdf5ed67f3990f4a296fb8320be5cead158MD52TEXTjoseli_vieira_etal_IOC_2013.pdf.txtjoseli_vieira_etal_IOC_2013.pdf.txtExtracted texttext/plain42813https://www.arca.fiocruz.br/bitstream/icict/11676/3/joseli_vieira_etal_IOC_2013.pdf.txtd24ea7cd082aa797042349f43005df33MD53icict/116762022-06-24 12:17:44.108oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
title A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
spellingShingle A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
Carlétti, Dyego
Tuberculosis
DNA Vaccine
Mycobacterium bovis
Mycobacterium bovis BCG prime DNA
Tuberculose
Mycobacterium bovis
Vacina BCG
title_short A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
title_full A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
title_fullStr A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
title_full_unstemmed A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
title_sort A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
author Carlétti, Dyego
author_facet Carlétti, Dyego
Fonseca, Denise Morais da
Gembre, Ana Flavia
Masson, Ana Paula
Campos, Lívia Weijenborg
Leite, Luciana C. C.
Pires, Andréa Rodrigues
Vieira, Joseli Lannes
Silva, Célio Lopes
Bonato, Vânia Luiza Deperon
Horn, Cynthia
author_role author
author2 Fonseca, Denise Morais da
Gembre, Ana Flavia
Masson, Ana Paula
Campos, Lívia Weijenborg
Leite, Luciana C. C.
Pires, Andréa Rodrigues
Vieira, Joseli Lannes
Silva, Célio Lopes
Bonato, Vânia Luiza Deperon
Horn, Cynthia
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carlétti, Dyego
Fonseca, Denise Morais da
Gembre, Ana Flavia
Masson, Ana Paula
Campos, Lívia Weijenborg
Leite, Luciana C. C.
Pires, Andréa Rodrigues
Vieira, Joseli Lannes
Silva, Célio Lopes
Bonato, Vânia Luiza Deperon
Horn, Cynthia
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
DNA Vaccine
Mycobacterium bovis
Mycobacterium bovis BCG prime DNA
topic Tuberculosis
DNA Vaccine
Mycobacterium bovis
Mycobacterium bovis BCG prime DNA
Tuberculose
Mycobacterium bovis
Vacina BCG
dc.subject.decs.pt_BR.fl_str_mv Tuberculose
Mycobacterium bovis
Vacina BCG
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-09-21T17:25:10Z
dc.date.available.fl_str_mv 2015-09-21T17:25:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11676
dc.identifier.doi.pt_BR.fl_str_mv 10.1128/CVI.00148-13
identifier_str_mv CARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.
10.1128/CVI.00148-13
url https://www.arca.fiocruz.br/handle/icict/11676
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11676/1/license.txt
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