Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24270 |
Resumo: | Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95â—¦C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential. |
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Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessmentAloesinAloevera green rindAntimicrobial activityAntioxidant activityAqueous glycolic solventsCytotoxicityProcess optimizationAloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95â—¦C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). M. Añibarro-Ortega and A.K. Molina are grateful to FCT, P.I., for their PhD studentships (2020.06297.BD and 2020.06231.BD, respectively), J. Pinela for the contract (CEECIND/01011/2018) through the individual scientific employment program-contract, and L. Barros and R.C. Calhelha through the institutional scientific employment program-contract. To the Project AllNat—POCI-01-0145- FEDER-030463 (PTDC/EQU-EPQ/30463/2017), funded by FEDER funds through COMPETE2020— Programa Operacional Competitividade e Internacionalização (POCI)—and by national funds through FCT/MCTES. To the FEDER-Interreg España-Portugal programme for financial support through the project TRANSCoLAB 0612_TRANS_CO_LAB_2_P and to ERDF through the Regional Operational Program North 2020, within the scope of the Project GreenHealth—Norte-01-0145- FEDER-000042. The authors are also grateful to the Ministry of Education, Science and Technological Development, Republic of Serbia, grant No. 173032.Biblioteca Digital do IPBAñibarro-Ortega, MikelPinela, JoséĆirić, AnaLopes, ElsaMolina, Adriana K.Calhelha, Ricardo C.Soković, MarinaFerreira, OlgaFerreira, Isabel C.F.R.Barros, Lillian2018-01-19T10:00:00Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/24270engAñibarro-Ortega, Mikel; Pinela, José; Ćirić, Ana; Lopes, Elsa; Molina, Adriana K.; Calhelha, Ricardo C.; Soković, Marina; Ferreira, Olga; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment. Biology. ISSN 2079-7737. 10:10, p. 1-162079-773710.3390/biology10100951info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:15Zoai:bibliotecadigital.ipb.pt:10198/24270Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:07.208980Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
title |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
spellingShingle |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment Añibarro-Ortega, Mikel Aloesin Aloevera green rind Antimicrobial activity Antioxidant activity Aqueous glycolic solvents Cytotoxicity Process optimization |
title_short |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
title_full |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
title_fullStr |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
title_full_unstemmed |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
title_sort |
Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment |
author |
Añibarro-Ortega, Mikel |
author_facet |
Añibarro-Ortega, Mikel Pinela, José Ćirić, Ana Lopes, Elsa Molina, Adriana K. Calhelha, Ricardo C. Soković, Marina Ferreira, Olga Ferreira, Isabel C.F.R. Barros, Lillian |
author_role |
author |
author2 |
Pinela, José Ćirić, Ana Lopes, Elsa Molina, Adriana K. Calhelha, Ricardo C. Soković, Marina Ferreira, Olga Ferreira, Isabel C.F.R. Barros, Lillian |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Añibarro-Ortega, Mikel Pinela, José Ćirić, Ana Lopes, Elsa Molina, Adriana K. Calhelha, Ricardo C. Soković, Marina Ferreira, Olga Ferreira, Isabel C.F.R. Barros, Lillian |
dc.subject.por.fl_str_mv |
Aloesin Aloevera green rind Antimicrobial activity Antioxidant activity Aqueous glycolic solvents Cytotoxicity Process optimization |
topic |
Aloesin Aloevera green rind Antimicrobial activity Antioxidant activity Aqueous glycolic solvents Cytotoxicity Process optimization |
description |
Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95â—¦C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/24270 |
url |
http://hdl.handle.net/10198/24270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Añibarro-Ortega, Mikel; Pinela, José; Ćirić, Ana; Lopes, Elsa; Molina, Adriana K.; Calhelha, Ricardo C.; Soković, Marina; Ferreira, Olga; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Extraction of aloesin from aloe vera rind using alternative green solvents: Process optimization and biological activity assessment. Biology. ISSN 2079-7737. 10:10, p. 1-16 2079-7737 10.3390/biology10100951 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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