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Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Carla Aparecida
Data de Publicação: 2017
Outros Autores: Soares, Iberê Cauduro, Penna, Valter, Squire, Jeremy, Reis, R. M., Silva, Sandra Regina Morini da, Carvalho, Isabela de, Camparoto, Marjori Leiva, Silva, Maicon Fernando Zanon da, Longatto, Adhemar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/49950
Resumo: The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.
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spelling Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample studyChondrosarcomaCentrosome amplificationPrimary cell cultureCiências Médicas::Medicina BásicaScience & TechnologyThe genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.info:eu-repo/semantics/publishedVersionSpandidos PublicationsUniversidade do MinhoPinheiro, Carla AparecidaSoares, Iberê CauduroPenna, ValterSquire, JeremyReis, R. M.Silva, Sandra Regina Morini daCarvalho, Isabela deCamparoto, Marjori LeivaSilva, Maicon Fernando Zanon daLongatto, Adhemar2017-01-182017-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/49950engPinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M. V., da Silva, S. R. M., ... & Longatto Filho, A. (2017). Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncology letters, 13(3), 1835-18351792-10741792-108210.3892/ol.2017.5633https://www.spandidos-publications.com/10.3892/ol.2017.5633?text=abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:32Zoai:repositorium.sdum.uminho.pt:1822/49950Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:21.133361Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
title Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
spellingShingle Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
Pinheiro, Carla Aparecida
Chondrosarcoma
Centrosome amplification
Primary cell culture
Ciências Médicas::Medicina Básica
Science & Technology
title_short Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
title_full Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
title_fullStr Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
title_full_unstemmed Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
title_sort Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
author Pinheiro, Carla Aparecida
author_facet Pinheiro, Carla Aparecida
Soares, Iberê Cauduro
Penna, Valter
Squire, Jeremy
Reis, R. M.
Silva, Sandra Regina Morini da
Carvalho, Isabela de
Camparoto, Marjori Leiva
Silva, Maicon Fernando Zanon da
Longatto, Adhemar
author_role author
author2 Soares, Iberê Cauduro
Penna, Valter
Squire, Jeremy
Reis, R. M.
Silva, Sandra Regina Morini da
Carvalho, Isabela de
Camparoto, Marjori Leiva
Silva, Maicon Fernando Zanon da
Longatto, Adhemar
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pinheiro, Carla Aparecida
Soares, Iberê Cauduro
Penna, Valter
Squire, Jeremy
Reis, R. M.
Silva, Sandra Regina Morini da
Carvalho, Isabela de
Camparoto, Marjori Leiva
Silva, Maicon Fernando Zanon da
Longatto, Adhemar
dc.subject.por.fl_str_mv Chondrosarcoma
Centrosome amplification
Primary cell culture
Ciências Médicas::Medicina Básica
Science & Technology
topic Chondrosarcoma
Centrosome amplification
Primary cell culture
Ciências Médicas::Medicina Básica
Science & Technology
description The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-18
2017-01-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/49950
url http://hdl.handle.net/1822/49950
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M. V., da Silva, S. R. M., ... & Longatto Filho, A. (2017). Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncology letters, 13(3), 1835-1835
1792-1074
1792-1082
10.3892/ol.2017.5633
https://www.spandidos-publications.com/10.3892/ol.2017.5633?text=abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Spandidos Publications
publisher.none.fl_str_mv Spandidos Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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