Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study
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Publication Date: | 2017 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/49950 |
Summary: | The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor. |
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Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample studyChondrosarcomaCentrosome amplificationPrimary cell cultureCiências Médicas::Medicina BásicaScience & TechnologyThe genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.info:eu-repo/semantics/publishedVersionSpandidos PublicationsUniversidade do MinhoPinheiro, Carla AparecidaSoares, Iberê CauduroPenna, ValterSquire, JeremyReis, R. M.Silva, Sandra Regina Morini daCarvalho, Isabela deCamparoto, Marjori LeivaSilva, Maicon Fernando Zanon daLongatto, Adhemar2017-01-182017-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/49950engPinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M. V., da Silva, S. R. M., ... & Longatto Filho, A. (2017). Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncology letters, 13(3), 1835-18351792-10741792-108210.3892/ol.2017.5633https://www.spandidos-publications.com/10.3892/ol.2017.5633?text=abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:32Zoai:repositorium.sdum.uminho.pt:1822/49950Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:21.133361Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
title |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
spellingShingle |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study Pinheiro, Carla Aparecida Chondrosarcoma Centrosome amplification Primary cell culture Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
title_full |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
title_fullStr |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
title_full_unstemmed |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
title_sort |
Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study |
author |
Pinheiro, Carla Aparecida |
author_facet |
Pinheiro, Carla Aparecida Soares, Iberê Cauduro Penna, Valter Squire, Jeremy Reis, R. M. Silva, Sandra Regina Morini da Carvalho, Isabela de Camparoto, Marjori Leiva Silva, Maicon Fernando Zanon da Longatto, Adhemar |
author_role |
author |
author2 |
Soares, Iberê Cauduro Penna, Valter Squire, Jeremy Reis, R. M. Silva, Sandra Regina Morini da Carvalho, Isabela de Camparoto, Marjori Leiva Silva, Maicon Fernando Zanon da Longatto, Adhemar |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pinheiro, Carla Aparecida Soares, Iberê Cauduro Penna, Valter Squire, Jeremy Reis, R. M. Silva, Sandra Regina Morini da Carvalho, Isabela de Camparoto, Marjori Leiva Silva, Maicon Fernando Zanon da Longatto, Adhemar |
dc.subject.por.fl_str_mv |
Chondrosarcoma Centrosome amplification Primary cell culture Ciências Médicas::Medicina Básica Science & Technology |
topic |
Chondrosarcoma Centrosome amplification Primary cell culture Ciências Médicas::Medicina Básica Science & Technology |
description |
The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-gamma-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with.-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-18 2017-01-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/49950 |
url |
http://hdl.handle.net/1822/49950 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M. V., da Silva, S. R. M., ... & Longatto Filho, A. (2017). Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncology letters, 13(3), 1835-1835 1792-1074 1792-1082 10.3892/ol.2017.5633 https://www.spandidos-publications.com/10.3892/ol.2017.5633?text=abstract |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Spandidos Publications |
publisher.none.fl_str_mv |
Spandidos Publications |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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