Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34456 |
Resumo: | The purification of value-added compounds by three phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid-liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Accordingly, we have here investigated the use of bio-based glycine-betaine ionic liquids (IL) directly with honey, a carbohydrate-rich matrix, as phase-forming components of TPP systems. These ILTPP systems were applied in the purification of Major Royal Jelly Proteins (MRJPs) from honey. The results obtained show that MRJPs mostly precipitate in the ILTPP interphase, with a recovery yield ranging between 82.8 and 97.3%. In particular, MRJP1 can be obtained with a purity level up to 90.1%. Furthermore, these systems allow the simultaneous separation of antioxidants and carbohydrates to different liquid phases. The proposed approach allows the separation of proteins, antioxidants, and carbohydrates from honey in a single step, while using only ILs and a real carbohydrate-rich matrix, being thus sustainable TPP processes. |
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Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioningIonic liquidThree-phase partitioningMajor Royal Jelly ProteinsAntioxidantsExtractionPurificationThe purification of value-added compounds by three phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid-liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Accordingly, we have here investigated the use of bio-based glycine-betaine ionic liquids (IL) directly with honey, a carbohydrate-rich matrix, as phase-forming components of TPP systems. These ILTPP systems were applied in the purification of Major Royal Jelly Proteins (MRJPs) from honey. The results obtained show that MRJPs mostly precipitate in the ILTPP interphase, with a recovery yield ranging between 82.8 and 97.3%. In particular, MRJP1 can be obtained with a purity level up to 90.1%. Furthermore, these systems allow the simultaneous separation of antioxidants and carbohydrates to different liquid phases. The proposed approach allows the separation of proteins, antioxidants, and carbohydrates from honey in a single step, while using only ILs and a real carbohydrate-rich matrix, being thus sustainable TPP processes.American Chemical Society2023-07-25T00:00:00Z2022-07-25T00:00:00Z2022-07-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34456eng10.1021/acssuschemeng.2c01782Pereira, Matheus M.Pedro, Sónia N.Quental, Maria V.Mohamadou, AminouCoutinho, João A. P.Freire, Mara G.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-17T04:14:18ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
title |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
spellingShingle |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning Pereira, Matheus M. Ionic liquid Three-phase partitioning Major Royal Jelly Proteins Antioxidants Extraction Purification |
title_short |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
title_full |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
title_fullStr |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
title_full_unstemmed |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
title_sort |
Integrated approach to extract and purify proteins from honey by ionic liquid-based three-phase partitioning |
author |
Pereira, Matheus M. |
author_facet |
Pereira, Matheus M. Pedro, Sónia N. Quental, Maria V. Mohamadou, Aminou Coutinho, João A. P. Freire, Mara G. |
author_role |
author |
author2 |
Pedro, Sónia N. Quental, Maria V. Mohamadou, Aminou Coutinho, João A. P. Freire, Mara G. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Matheus M. Pedro, Sónia N. Quental, Maria V. Mohamadou, Aminou Coutinho, João A. P. Freire, Mara G. |
dc.subject.por.fl_str_mv |
Ionic liquid Three-phase partitioning Major Royal Jelly Proteins Antioxidants Extraction Purification |
topic |
Ionic liquid Three-phase partitioning Major Royal Jelly Proteins Antioxidants Extraction Purification |
description |
The purification of value-added compounds by three phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid-liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Accordingly, we have here investigated the use of bio-based glycine-betaine ionic liquids (IL) directly with honey, a carbohydrate-rich matrix, as phase-forming components of TPP systems. These ILTPP systems were applied in the purification of Major Royal Jelly Proteins (MRJPs) from honey. The results obtained show that MRJPs mostly precipitate in the ILTPP interphase, with a recovery yield ranging between 82.8 and 97.3%. In particular, MRJP1 can be obtained with a purity level up to 90.1%. Furthermore, these systems allow the simultaneous separation of antioxidants and carbohydrates to different liquid phases. The proposed approach allows the separation of proteins, antioxidants, and carbohydrates from honey in a single step, while using only ILs and a real carbohydrate-rich matrix, being thus sustainable TPP processes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-25T00:00:00Z 2022-07-25 2023-07-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34456 |
url |
http://hdl.handle.net/10773/34456 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1021/acssuschemeng.2c01782 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303585196867584 |