O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro

• Autor(a) principal: Ragonezi, Carla
• Data de Publicação: 2013
• Outros Autores: Teixeira, Dora, Caldeira, Ana Teresa, Martins, Maria do Rosário, Santos Silva, Celeste, Ganhão, Elsa, Klimaszewska, Krystyna, Zavattieri, Maria Amely
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10174/9338; https://doi.org/JOUR T1 - O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro AU - Ragonezi, C. AU - Teixeira, D. AU - Caldeira, A.T. AU - Martins, M.R. AU - Santos-Silva, C. AU - Ganhão, E. AU - Klimaszewska, K. AU - Zavattieri, M.A. Y1 - 2013/08/16 PY - 2013 N1 - doi: 10.1080/17429145.2013.831489 DO - 10.1080/17429145.2013.831489 T2 - Journal of Plant Interactions JF - Journal of Plant Interactions JO - Journal of Plant Interactions SP - 1 EP - 9 PB - Taylor & Francis SN - 1742-9145 M3 - doi: 10.1080/17429145.2013.831489 UR - http://dx.doi.org/10.1080/17429145.2013.831489 Y2 - 2013/09/20 ER -; https://doi.org/10.1080/17429145.2013.831489
• topic: Adventitious rooting; Co-cultivation; Ectomycorrhiza; Phenolics; Pre-colonization; Stone pine

During ectomycorrhizal (ECM) establishment, biochemical signals lead to the development of complex structures in both the plant and the fungus that ultimately result in the formation of an ectomycorrhiza. The cross-talk between partners begins before physical contact. Our objective was to investigate the chemical nature of the signals during the first stages of in vitro mycorrhization of Pinus pinea with Pisolithus arhizus. For this purpose a double-phase solid–liquid medium was expressly developed for the co-culture in order to simplify the extraction and further molecules analysis. O-coumaric acid ester was identified using HPLC–UV and LC–DAD–MS on the second day of co-culture and its presence was detected for up to 10 days. These results contribute to the characterization of biochemical signals during pre-colonization involving conifer species and an ECM fungus, and demonstrate the suitability of the double-phase medium developed for the growth of both organisms and for the analysis of released chemical mediators.