Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots

Detalhes bibliográficos
Autor(a) principal: Ragonezi, Carla
Data de Publicação: 2018
Outros Autores: Zavattieri, Maria Amely
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/23383
Resumo: Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.
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spelling Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine RootsEctomycorrhizaCryostatMycorrhiza-like structuresHartig-netMicroscopyAdventitious rootingSeveral studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.MDPI Publications2018-09-07T14:09:54Z2018-09-072018-09-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/23383http://hdl.handle.net/10174/23383engRagonezi, Carla & Zavattieri Maria Amely (2018) Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots. Methods Protoc. 2018, 1(3), 34; doi: 10.3390/mps1030034http://www.mdpi.com/2409-9279/1/3/34/htmMethods and ProtocolsICT - Artigos em revistas internacionais com arbitragem científicacarla.ragonezi@staff.uma.ptmazavattieri@gmail.com544doi: 10.3390/mps1030034Ragonezi, CarlaZavattieri, Maria Amelyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:15:32Zoai:dspace.uevora.pt:10174/23383Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:16.510887Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
title Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
spellingShingle Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
Ragonezi, Carla
Ectomycorrhiza
Cryostat
Mycorrhiza-like structures
Hartig-net
Microscopy
Adventitious rooting
title_short Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
title_full Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
title_fullStr Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
title_full_unstemmed Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
title_sort Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots
author Ragonezi, Carla
author_facet Ragonezi, Carla
Zavattieri, Maria Amely
author_role author
author2 Zavattieri, Maria Amely
author2_role author
dc.contributor.author.fl_str_mv Ragonezi, Carla
Zavattieri, Maria Amely
dc.subject.por.fl_str_mv Ectomycorrhiza
Cryostat
Mycorrhiza-like structures
Hartig-net
Microscopy
Adventitious rooting
topic Ectomycorrhiza
Cryostat
Mycorrhiza-like structures
Hartig-net
Microscopy
Adventitious rooting
description Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-07T14:09:54Z
2018-09-07
2018-09-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/23383
http://hdl.handle.net/10174/23383
url http://hdl.handle.net/10174/23383
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ragonezi, Carla & Zavattieri Maria Amely (2018) Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots. Methods Protoc. 2018, 1(3), 34; doi: 10.3390/mps1030034
http://www.mdpi.com/2409-9279/1/3/34/htm
Methods and Protocols
ICT - Artigos em revistas internacionais com arbitragem científica
carla.ragonezi@staff.uma.pt
mazavattieri@gmail.com
544
doi: 10.3390/mps1030034
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI Publications
publisher.none.fl_str_mv MDPI Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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