Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots

Detalhes bibliográficos
Autor(a) principal: Ragonezi, Carla
Data de Publicação: 2018
Outros Autores: Zavattieri, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/4046
Resumo: Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.
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spelling Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine rootsEctomycorrhizaMycorrhiza-like structuresStone pineAdventitious rootsHartig net.Escola Superior de Tecnologias e GestãoSeveral studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.MDPIDigitUMaRagonezi, CarlaZavattieri, Maria2022-02-04T15:33:09Z2018-01-01T00:00:00Z2018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4046engRagonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps103003410.3390/mps1030034info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:57:10Zoai:digituma.uma.pt:10400.13/4046Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:43.994363Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
title Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
spellingShingle Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
Ragonezi, Carla
Ectomycorrhiza
Mycorrhiza-like structures
Stone pine
Adventitious roots
Hartig net
.
Escola Superior de Tecnologias e Gestão
title_short Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
title_full Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
title_fullStr Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
title_full_unstemmed Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
title_sort Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
author Ragonezi, Carla
author_facet Ragonezi, Carla
Zavattieri, Maria
author_role author
author2 Zavattieri, Maria
author2_role author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Ragonezi, Carla
Zavattieri, Maria
dc.subject.por.fl_str_mv Ectomycorrhiza
Mycorrhiza-like structures
Stone pine
Adventitious roots
Hartig net
.
Escola Superior de Tecnologias e Gestão
topic Ectomycorrhiza
Mycorrhiza-like structures
Stone pine
Adventitious roots
Hartig net
.
Escola Superior de Tecnologias e Gestão
description Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01T00:00:00Z
2018-01-01T00:00:00Z
2022-02-04T15:33:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/4046
url http://hdl.handle.net/10400.13/4046
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ragonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps1030034
10.3390/mps1030034
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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