Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

• Main Author: da Costa, Paulo J.
• Publication Date: 2019
• Other Authors: Menezes, Juliane, Saramago, Margarida, García-Moreno, Juan F., Santos, Hugo A., Gama-Carvalho, Margarida, Arraiano, Cecília M., Viegas, Sandra C., Romão, Luísa
• Format: Article
• Language: eng
• Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Download full: http://hdl.handle.net/10400.18/6501
• topic: DIS3L2; NMD; NMD-targets; UPF1; mRNA Degradation; mRNA Surveillance; Doenças Genéticas; Genómica Funcional e Estrutural

In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.