Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/113016 |
Resumo: | Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5–100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity – lactate dehydrogenase assay, analysis of cell cycle and cell death production – and genotoxicity – H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells. |
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Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytesIron oxide nanoparticlesNeurotoxicityCytotoxicityGenotoxicityIron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5–100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity – lactate dehydrogenase assay, analysis of cell cycle and cell death production – and genotoxicity – H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells.20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/113016eng0278-691510.1016/j.fct.2018.04.058Fernández-Bertólez, NCosta, CBrandão, FKiliç, GDuarte, JATeixeira, JPPásaro, EValdiglesias, VLaffon, Binfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:47:26Zoai:repositorio-aberto.up.pt:10216/113016Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:47:41.289701Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
title |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
spellingShingle |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes Fernández-Bertólez, N Iron oxide nanoparticles Neurotoxicity Cytotoxicity Genotoxicity |
title_short |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
title_full |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
title_fullStr |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
title_full_unstemmed |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
title_sort |
Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes |
author |
Fernández-Bertólez, N |
author_facet |
Fernández-Bertólez, N Costa, C Brandão, F Kiliç, G Duarte, JA Teixeira, JP Pásaro, E Valdiglesias, V Laffon, B |
author_role |
author |
author2 |
Costa, C Brandão, F Kiliç, G Duarte, JA Teixeira, JP Pásaro, E Valdiglesias, V Laffon, B |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernández-Bertólez, N Costa, C Brandão, F Kiliç, G Duarte, JA Teixeira, JP Pásaro, E Valdiglesias, V Laffon, B |
dc.subject.por.fl_str_mv |
Iron oxide nanoparticles Neurotoxicity Cytotoxicity Genotoxicity |
topic |
Iron oxide nanoparticles Neurotoxicity Cytotoxicity Genotoxicity |
description |
Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5–100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity – lactate dehydrogenase assay, analysis of cell cycle and cell death production – and genotoxicity – H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/113016 |
url |
http://hdl.handle.net/10216/113016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0278-6915 10.1016/j.fct.2018.04.058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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