Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/693 |
Resumo: | Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I. |
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Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodellingEpigeneticsmeiosisOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I.Harvard Medical School; NIH/NIGMS grants: R01-GM084947, RO1 1119484-1-68857, R01GM112783); Fundação para a Ciência e a Tecnologia grant: (PTDC/BEX-BID/0395/2014); SUNY Presidential Innovative Award; Pew Biomedical Scholars Program.Nature Publishing GroupARCANavarro-Costa, PauloMcCarthy, AliciaPrudêncio, PedroGreer, ChristinaGuilgur, Leonardo G.Becker, Jörg D.Secombe, JulieRangan, PrashanthMartinho, Rui G.2016-08-23T15:06:46Z2016-08-102016-08-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/693engNavarro-Costa, P. et al. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nat. Commun. 7:12331 doi: 10.1038/ncomms12331 (2016).10.1038/ncomms12331info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:04Zoai:arca.igc.gulbenkian.pt:10400.7/693Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:55.551114Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
spellingShingle |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling Navarro-Costa, Paulo Epigenetics meiosis |
title_short |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_full |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_fullStr |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_full_unstemmed |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
title_sort |
Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling |
author |
Navarro-Costa, Paulo |
author_facet |
Navarro-Costa, Paulo McCarthy, Alicia Prudêncio, Pedro Greer, Christina Guilgur, Leonardo G. Becker, Jörg D. Secombe, Julie Rangan, Prashanth Martinho, Rui G. |
author_role |
author |
author2 |
McCarthy, Alicia Prudêncio, Pedro Greer, Christina Guilgur, Leonardo G. Becker, Jörg D. Secombe, Julie Rangan, Prashanth Martinho, Rui G. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Navarro-Costa, Paulo McCarthy, Alicia Prudêncio, Pedro Greer, Christina Guilgur, Leonardo G. Becker, Jörg D. Secombe, Julie Rangan, Prashanth Martinho, Rui G. |
dc.subject.por.fl_str_mv |
Epigenetics meiosis |
topic |
Epigenetics meiosis |
description |
Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-23T15:06:46Z 2016-08-10 2016-08-10T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/693 |
url |
http://hdl.handle.net/10400.7/693 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Navarro-Costa, P. et al. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nat. Commun. 7:12331 doi: 10.1038/ncomms12331 (2016). 10.1038/ncomms12331 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130574693072896 |