Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus

Detalhes bibliográficos
Autor(a) principal: Gandra,Eliezer Avila
Data de Publicação: 2011
Outros Autores: Fernandez,Maria Aparecida, Silva,Jorge Adolfo, Silva,Wladimir Padilha da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019
Resumo: The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.
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spelling Standardization of a multiplex PCR for the identification of coagulase-positive StaphylococcusS. aureusS. hyicusS. intermediusPCRnuc geneThe enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019Food Science and Technology v.31 n.4 2011reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612011000400019info:eu-repo/semantics/openAccessGandra,Eliezer AvilaFernandez,Maria AparecidaSilva,Jorge AdolfoSilva,Wladimir Padilha daeng2012-02-06T00:00:00Zoai:scielo:S0101-20612011000400019Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2012-02-06T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
title Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
spellingShingle Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
Gandra,Eliezer Avila
S. aureus
S. hyicus
S. intermedius
PCR
nuc gene
title_short Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
title_full Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
title_fullStr Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
title_full_unstemmed Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
title_sort Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
author Gandra,Eliezer Avila
author_facet Gandra,Eliezer Avila
Fernandez,Maria Aparecida
Silva,Jorge Adolfo
Silva,Wladimir Padilha da
author_role author
author2 Fernandez,Maria Aparecida
Silva,Jorge Adolfo
Silva,Wladimir Padilha da
author2_role author
author
author
dc.contributor.author.fl_str_mv Gandra,Eliezer Avila
Fernandez,Maria Aparecida
Silva,Jorge Adolfo
Silva,Wladimir Padilha da
dc.subject.por.fl_str_mv S. aureus
S. hyicus
S. intermedius
PCR
nuc gene
topic S. aureus
S. hyicus
S. intermedius
PCR
nuc gene
description The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 10² CFU.mL-1.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-20612011000400019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.31 n.4 2011
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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