Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture

Detalhes bibliográficos
Autor(a) principal: Gomes, Lucas Roberto Pereira
Data de Publicação: 2015
Outros Autores: Franceschi, Cristina do Rosário Batista, Ribas, Luciana Lopes Fortes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276
Resumo: An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival). 
id UEM-1_7ec8699bd5540a22ac594b631b1da109
oai_identifier_str oai:periodicos.uem.br/ojs:article/27276
network_acronym_str UEM-1
network_name_str Acta Scientiarum Biological Sciences
repository_id_str
spelling Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer cultureepiphytic orchidin vitro propagationprotocormsprotocorm-like body2.03.00.00-0An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival). Universidade Estadual De Maringá2015-08-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2727610.4025/actascibiolsci.v37i2.27276Acta Scientiarum. Biological Sciences; Vol 37 No 2 (2015); 143-149Acta Scientiarum. Biological Sciences; v. 37 n. 2 (2015); 143-1491807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276/pdf_49Gomes, Lucas Roberto PereiraFranceschi, Cristina do Rosário BatistaRibas, Luciana Lopes Fortesinfo:eu-repo/semantics/openAccess2015-08-06T15:38:50Zoai:periodicos.uem.br/ojs:article/27276Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2015-08-06T15:38:50Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
title Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
spellingShingle Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
Gomes, Lucas Roberto Pereira
epiphytic orchid
in vitro propagation
protocorms
protocorm-like body
2.03.00.00-0
title_short Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
title_full Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
title_fullStr Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
title_full_unstemmed Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
title_sort Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
author Gomes, Lucas Roberto Pereira
author_facet Gomes, Lucas Roberto Pereira
Franceschi, Cristina do Rosário Batista
Ribas, Luciana Lopes Fortes
author_role author
author2 Franceschi, Cristina do Rosário Batista
Ribas, Luciana Lopes Fortes
author2_role author
author
dc.contributor.author.fl_str_mv Gomes, Lucas Roberto Pereira
Franceschi, Cristina do Rosário Batista
Ribas, Luciana Lopes Fortes
dc.subject.por.fl_str_mv epiphytic orchid
in vitro propagation
protocorms
protocorm-like body
2.03.00.00-0
topic epiphytic orchid
in vitro propagation
protocorms
protocorm-like body
2.03.00.00-0
description An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival). 
publishDate 2015
dc.date.none.fl_str_mv 2015-08-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276
10.4025/actascibiolsci.v37i2.27276
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276
identifier_str_mv 10.4025/actascibiolsci.v37i2.27276
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276/pdf_49
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 37 No 2 (2015); 143-149
Acta Scientiarum. Biological Sciences; v. 37 n. 2 (2015); 143-149
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
_version_ 1799317396357382144