Antioxidante na viabilidade do sêmen equino congelado e refrigerado

• Autor(a) principal: OLIVEIRA, Rodrigo Arruda de
• Data de Publicação: 2011
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/1113
• topic: Cisteína; espermatozóide; Garanhão; glutationa; recursos genéticos; 1. Antioxidantes- Sêmen 2. Equinos; antioxidants, equine, semen; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL

In the equine world is increasing the use of frozen semen and cooled due to the recent release of its use for most of breeders' associations. However, the fertility of frozen semen is still low and this hinders their widespread use. The objective was to evaluate the effect In vitro addition of glutathione on sperm concentrations in five of 12 stallions in different protocols of freezing, and I Experiment (EI): automated freezing immediately after harvest and Experiment II (EII): automated freezing (MRI) and manual (RG) after passive cooling 24 h at 16 ° C. The variables assessed were total and progressive motility, vigor, plasma membrane integrity and acrosomal. The addition of glutathione in concentration of 2.5 mM in the midst of freezing preserves the total motility, significantly increases the motility and integrity of plasma membrane, both for semen frozen immediately after harvesting and for the refrigerated and frozen manually. Concentrations exceeding 2.5 mM were deleterious to sperm in different freezing protocols, with or without passive cooling. In chapter 3 aimed to evaluate the in vitro effect of the addition of cysteine ​​and glutathione in seven treatments on sperm from 12 stallions in protocol passive cooling to 16 ° C for 36h. The variables assessed were total motility and progressive force and plasma membrane integrity and acrosomal in seven different times (0, 6, 12, 18, ​​24, 30 and 36h). For discussion of data were taken into account only the values ​​of the moments 1, 3, 5 and 7 (0, 12, 24 and 36h). The concentration of 1.0 mM cysteine ​​was more effective in protection of sperm cells in the trading system of the passive cooling 16 º C for 36h, with higher values ​​of motility and membrane integrity.