Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3762 https://doi.org/10.1016/j.mib.2012.05.002 |
Resumo: | Visceral leishmaniasis is a major health problem in Latina America, as well as the Mediterranean region of Europe and Asia. We aimed to develop a vaccine against visceral leishmaniasis targeting the intracellular amastigotes, which is the parasite stage that persists throughout infections with Leishmania parasites. With this in mind, we identified an amastigote specific antigen (A2) that contains an immunogenic epitope for CD4+ T helper (Th) cells and multiple repetitive units encoding CD8+ cytotoxic T lymphocyte (CTL) epitopes. Vaccine formulations containing the recombinant A2 associated with saponin, alum and IL-12 or expressed by attenuated adenovirus were shown to be protective in mice, dogs and nonhuman-primates. We are currently identifying novel amastigote specific immunogenic proteins that could be aggregated to A2 to further improve the level of vaccineinduced cell-mediated immunity and protection against visceral leishmaniasis. |
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Fernandes, Ana PaulaCoelho, Eduardo Antônio FerrazCoelho, George Luiz Lins MachadoGrimaldi Junior, GabrielGazzinelli, Ricardo Tostes2014-11-11T17:10:07Z2014-11-11T17:10:07Z2012FERNANDES, A. P. Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. Current Opinion in Microbiology, v. 5, p. 12, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1369527412000562>. Acesso em: 01 set. 2014.1369-5274http://www.repositorio.ufop.br/handle/123456789/3762https://doi.org/10.1016/j.mib.2012.05.002Visceral leishmaniasis is a major health problem in Latina America, as well as the Mediterranean region of Europe and Asia. We aimed to develop a vaccine against visceral leishmaniasis targeting the intracellular amastigotes, which is the parasite stage that persists throughout infections with Leishmania parasites. With this in mind, we identified an amastigote specific antigen (A2) that contains an immunogenic epitope for CD4+ T helper (Th) cells and multiple repetitive units encoding CD8+ cytotoxic T lymphocyte (CTL) epitopes. Vaccine formulations containing the recombinant A2 associated with saponin, alum and IL-12 or expressed by attenuated adenovirus were shown to be protective in mice, dogs and nonhuman-primates. We are currently identifying novel amastigote specific immunogenic proteins that could be aggregated to A2 to further improve the level of vaccineinduced cell-mediated immunity and protection against visceral leishmaniasis.Visceral leishmaniasisVaccineMaking an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Current Opinion in Microbiology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3461450283724.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3762/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_Makingantiamastigote.pdfARTIGO_Makingantiamastigote.pdfapplication/pdf644854http://www.repositorio.ufop.br/bitstream/123456789/3762/1/ARTIGO_Makingantiamastigote.pdfd893d3ab27eee626930a5d3a9efacb30MD51123456789/37622019-05-06 14:06:31.282oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-06T18:06:31Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
title |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
spellingShingle |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. Fernandes, Ana Paula Visceral leishmaniasis Vaccine |
title_short |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
title_full |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
title_fullStr |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
title_full_unstemmed |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
title_sort |
Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. |
author |
Fernandes, Ana Paula |
author_facet |
Fernandes, Ana Paula Coelho, Eduardo Antônio Ferraz Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes |
author_role |
author |
author2 |
Coelho, Eduardo Antônio Ferraz Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Ana Paula Coelho, Eduardo Antônio Ferraz Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes |
dc.subject.por.fl_str_mv |
Visceral leishmaniasis Vaccine |
topic |
Visceral leishmaniasis Vaccine |
description |
Visceral leishmaniasis is a major health problem in Latina America, as well as the Mediterranean region of Europe and Asia. We aimed to develop a vaccine against visceral leishmaniasis targeting the intracellular amastigotes, which is the parasite stage that persists throughout infections with Leishmania parasites. With this in mind, we identified an amastigote specific antigen (A2) that contains an immunogenic epitope for CD4+ T helper (Th) cells and multiple repetitive units encoding CD8+ cytotoxic T lymphocyte (CTL) epitopes. Vaccine formulations containing the recombinant A2 associated with saponin, alum and IL-12 or expressed by attenuated adenovirus were shown to be protective in mice, dogs and nonhuman-primates. We are currently identifying novel amastigote specific immunogenic proteins that could be aggregated to A2 to further improve the level of vaccineinduced cell-mediated immunity and protection against visceral leishmaniasis. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-11-11T17:10:07Z |
dc.date.available.fl_str_mv |
2014-11-11T17:10:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, A. P. Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. Current Opinion in Microbiology, v. 5, p. 12, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1369527412000562>. Acesso em: 01 set. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3762 |
dc.identifier.issn.none.fl_str_mv |
1369-5274 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.mib.2012.05.002 |
identifier_str_mv |
FERNANDES, A. P. Making an anti-amastigote vaccine for visceral leishmaniasis : rational, update and perspectives. Current Opinion in Microbiology, v. 5, p. 12, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1369527412000562>. Acesso em: 01 set. 2014. 1369-5274 |
url |
http://www.repositorio.ufop.br/handle/123456789/3762 https://doi.org/10.1016/j.mib.2012.05.002 |
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eng |
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openAccess |
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