Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/37266 |
Resumo: | Considering the widespread consumption of milk powder by the general population as well as the lack of studies on the toxicity of such industrialized foods, this study evaluated the cytotoxic and genotoxic potential of powdered milk from four reputed companies in the food market of Brazil and other South American countries. Milk samples were evaluated in root meristem cells of Allium cepa L., at concentrations of 0.065 and 0.13 g/mL, for 24 and 48 hours of exposure; and by means of cell viability in culture of cells of normal lineage, via MTT test, for 24 hours, at concentrations of 0.016; 0.032; 0.065 and 0.13g/mL. The concentration 0.13 g/mL was the one suggested for consumption in all milk packages evaluated in this study. In A. cepa, we observed that the milks, at both concentrations and at the two exposure times investigated, reduced the cellular proliferation of root meristems demonstrating a significant cytotoxicity. Furthermore, 0.13g/mL milks at the exposure time of 24h induced an expressive frequency of cellular alterations in the plant tissue, showing to be genotoxic. In the in vitro evaluation, three milks at 0.065 g/mL and all milks at 0.13 g/mL have significantly reduced cell viability, proving to be cytotoxic to the analyzed cell culture. Therefore, under the studied conditions, the powdered milks evaluated caused significant genetic instability to the cells of the test systems used. |
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Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity Leites industrializados, tipo em pó, frente a bioensaios de avaliação de citotoxicidade e genotoxicidadepowdered milkcell divisionmitotic spindle changescell viabilitymeristematic tissueMTT test.Biological SciencesConsidering the widespread consumption of milk powder by the general population as well as the lack of studies on the toxicity of such industrialized foods, this study evaluated the cytotoxic and genotoxic potential of powdered milk from four reputed companies in the food market of Brazil and other South American countries. Milk samples were evaluated in root meristem cells of Allium cepa L., at concentrations of 0.065 and 0.13 g/mL, for 24 and 48 hours of exposure; and by means of cell viability in culture of cells of normal lineage, via MTT test, for 24 hours, at concentrations of 0.016; 0.032; 0.065 and 0.13g/mL. The concentration 0.13 g/mL was the one suggested for consumption in all milk packages evaluated in this study. In A. cepa, we observed that the milks, at both concentrations and at the two exposure times investigated, reduced the cellular proliferation of root meristems demonstrating a significant cytotoxicity. Furthermore, 0.13g/mL milks at the exposure time of 24h induced an expressive frequency of cellular alterations in the plant tissue, showing to be genotoxic. In the in vitro evaluation, three milks at 0.065 g/mL and all milks at 0.13 g/mL have significantly reduced cell viability, proving to be cytotoxic to the analyzed cell culture. Therefore, under the studied conditions, the powdered milks evaluated caused significant genetic instability to the cells of the test systems used.Devido o amplo consumo de leite em pó pela população em geral, bem como, a carência de estudos sobre a toxicidade de tais alimentos industrializados, objetivou-se na presente pesquisa avaliar o potencial citotóxico e genotóxico de leites em pó provenientes de quatro empresas de reconhecida atuação no mercado de alimentos brasileiro e de outros países da América do sul. As amostras de leite foram avaliadas em células meristemáticas de raízes de Allium cepa L., nas concentrações 0,065 e 0,13g/mL, por 24 e 48 horas de exposição; e por meio da viabilidade celular em cultura de células de linhagem normal, via teste MTT, por 24 horas, nas concentrações 0,016; 0,032; 0,065 e 0,13g/mL. A concentração 0,13 mL/kg foi a sugerida para consumo em todas embalagens de leites avaliados neste estudo. Em A. cepa, verificou-se que os leites, nas duas concentrações e nos dois tempos de análise considerados, reduziram a proliferação celular dos meristemas de raízes demonstrando citotoxicidade significativa. Ainda, os leites na concentração 0,13g/mLinduziram, no tempo de exposição 24h, frequência expressiva de alterações celulares ao tecido vegetal, mostrando-se genotóxicas. Na avaliação in vitro, três leites na concentração 0,065g/mL e todos na concentração 0,13g/mL reduziram significativamente a viabilidade celular mostrando-se citotóxicos a cultura de células analisada. Portanto, nas condições de estudo estabelecidas, os leites em pó avaliados causaram significativa instabilidade genética as células dos sistemas testes utilizados.EDUFU2017-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3726610.14393/BJ-v33n6a2017-37266Bioscience Journal ; Vol. 33 No. 6 (2017): Nov./Dec.; 1622-1631Bioscience Journal ; v. 33 n. 6 (2017): Nov./Dec.; 1622-16311981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/37266/21208Brazil; ContemporaryCopyright (c) 2017 Rayssa Aléxia Martins Moura, Marcia Maria Mendes Marques, Helena Mendes Pereira, Ila Monize Sousa Sales, Fabelina Karolyne Sousa Santos, Maria Izabel Florindo Guedes, Ana Paula Peronhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMoura, Rayssa Aléxia MartinsMarques, Marcia Maria MendesPereira, Helena MendesSales, Ila Monize SousaSantos, Fabelina Karolyne SousaGuedes, Maria Izabel FlorindoPeron, Ana Paula2022-02-12T16:03:41Zoai:ojs.www.seer.ufu.br:article/37266Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-12T16:03:41Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity Leites industrializados, tipo em pó, frente a bioensaios de avaliação de citotoxicidade e genotoxicidade |
title |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
spellingShingle |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity Moura, Rayssa Aléxia Martins powdered milk cell division mitotic spindle changes cell viability meristematic tissue MTT test. Biological Sciences |
title_short |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
title_full |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
title_fullStr |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
title_full_unstemmed |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
title_sort |
Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity |
author |
Moura, Rayssa Aléxia Martins |
author_facet |
Moura, Rayssa Aléxia Martins Marques, Marcia Maria Mendes Pereira, Helena Mendes Sales, Ila Monize Sousa Santos, Fabelina Karolyne Sousa Guedes, Maria Izabel Florindo Peron, Ana Paula |
author_role |
author |
author2 |
Marques, Marcia Maria Mendes Pereira, Helena Mendes Sales, Ila Monize Sousa Santos, Fabelina Karolyne Sousa Guedes, Maria Izabel Florindo Peron, Ana Paula |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Moura, Rayssa Aléxia Martins Marques, Marcia Maria Mendes Pereira, Helena Mendes Sales, Ila Monize Sousa Santos, Fabelina Karolyne Sousa Guedes, Maria Izabel Florindo Peron, Ana Paula |
dc.subject.por.fl_str_mv |
powdered milk cell division mitotic spindle changes cell viability meristematic tissue MTT test. Biological Sciences |
topic |
powdered milk cell division mitotic spindle changes cell viability meristematic tissue MTT test. Biological Sciences |
description |
Considering the widespread consumption of milk powder by the general population as well as the lack of studies on the toxicity of such industrialized foods, this study evaluated the cytotoxic and genotoxic potential of powdered milk from four reputed companies in the food market of Brazil and other South American countries. Milk samples were evaluated in root meristem cells of Allium cepa L., at concentrations of 0.065 and 0.13 g/mL, for 24 and 48 hours of exposure; and by means of cell viability in culture of cells of normal lineage, via MTT test, for 24 hours, at concentrations of 0.016; 0.032; 0.065 and 0.13g/mL. The concentration 0.13 g/mL was the one suggested for consumption in all milk packages evaluated in this study. In A. cepa, we observed that the milks, at both concentrations and at the two exposure times investigated, reduced the cellular proliferation of root meristems demonstrating a significant cytotoxicity. Furthermore, 0.13g/mL milks at the exposure time of 24h induced an expressive frequency of cellular alterations in the plant tissue, showing to be genotoxic. In the in vitro evaluation, three milks at 0.065 g/mL and all milks at 0.13 g/mL have significantly reduced cell viability, proving to be cytotoxic to the analyzed cell culture. Therefore, under the studied conditions, the powdered milks evaluated caused significant genetic instability to the cells of the test systems used. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/37266 10.14393/BJ-v33n6a2017-37266 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/37266 |
identifier_str_mv |
10.14393/BJ-v33n6a2017-37266 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/37266/21208 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 33 No. 6 (2017): Nov./Dec.; 1622-1631 Bioscience Journal ; v. 33 n. 6 (2017): Nov./Dec.; 1622-1631 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069077829648384 |