Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856 |
Resumo: | This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g mL-1, C at 0.075 g mL-1 and D at 0.037 g mL-1, significantly reduced the cellular viability of the cell culture exposed to the foods studied, being potentially toxic. The milk A was considered the most toxic, with LC50 of 0.031 g mL-1, and B as the least toxic, with LC50 of 0.15 g mL-1. Therefore, the milk evaluated caused significant instability in cells of the test systems used and were characterized as cytotoxic. |
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Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young childrenInfant formula; cell division; meristematic tissue; cell viability; LC50This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g mL-1, C at 0.075 g mL-1 and D at 0.037 g mL-1, significantly reduced the cellular viability of the cell culture exposed to the foods studied, being potentially toxic. The milk A was considered the most toxic, with LC50 of 0.031 g mL-1, and B as the least toxic, with LC50 of 0.15 g mL-1. Therefore, the milk evaluated caused significant instability in cells of the test systems used and were characterized as cytotoxic.Universidade Estadual De Maringá2020-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4685610.4025/actascibiolsci.v42i1.46856Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e46856Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e468561807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856/751375149336Copyright (c) 2020 Acta Scientiarum. Biological Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Geysa Janne SousaSantos, Fabelina Karollyne Silva dosMarques, Márcia Maria MendesPeron, Ana Paula2020-05-05T12:49:05Zoai:periodicos.uem.br/ojs:article/46856Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2020-05-05T12:49:05Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
title |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
spellingShingle |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children Silva, Geysa Janne Sousa Infant formula; cell division; meristematic tissue; cell viability; LC50 |
title_short |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
title_full |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
title_fullStr |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
title_full_unstemmed |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
title_sort |
Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children |
author |
Silva, Geysa Janne Sousa |
author_facet |
Silva, Geysa Janne Sousa Santos, Fabelina Karollyne Silva dos Marques, Márcia Maria Mendes Peron, Ana Paula |
author_role |
author |
author2 |
Santos, Fabelina Karollyne Silva dos Marques, Márcia Maria Mendes Peron, Ana Paula |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva, Geysa Janne Sousa Santos, Fabelina Karollyne Silva dos Marques, Márcia Maria Mendes Peron, Ana Paula |
dc.subject.por.fl_str_mv |
Infant formula; cell division; meristematic tissue; cell viability; LC50 |
topic |
Infant formula; cell division; meristematic tissue; cell viability; LC50 |
description |
This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g mL-1, C at 0.075 g mL-1 and D at 0.037 g mL-1, significantly reduced the cellular viability of the cell culture exposed to the foods studied, being potentially toxic. The milk A was considered the most toxic, with LC50 of 0.031 g mL-1, and B as the least toxic, with LC50 of 0.15 g mL-1. Therefore, the milk evaluated caused significant instability in cells of the test systems used and were characterized as cytotoxic. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856 10.4025/actascibiolsci.v42i1.46856 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856 |
identifier_str_mv |
10.4025/actascibiolsci.v42i1.46856 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856/751375149336 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e46856 Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e46856 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317397435318272 |