Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases?
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000492675 http://hdl.handle.net/11449/186887 |
Resumo: | The purpose of this cohort study was to identify associations between combined oral and bone disease phenotypes and genes present in cell regulatory pathways. The studied pathways play important roles in cellular growth, proliferation, differentiation, and homeostasis. DNA samples extracted from whole saliva of 3,912 individuals were genotyped and these data analyzed according to dental caries experience, periapical lesions, periodontitis, osteoporosis, or temporomandibular joint discomfort. Samples were obtained from the Dental Registry and DNA Repository project at the University of Pittsburgh. Twenty-seven polymorphisms in eight genes related to mTOR or endoplasmic reticulum stress pathways were selected for genotyping. Allele frequencies and Hardy-Weinberg equilibrium were calculated. Analyses were performed comparing genotypes between affected and unaffected individuals for each phenotype, as well as for the associated phenotypes combined. For all analyses, we used the software PLINK with an alpha of 0.002. Borderline associations with multiple variants of several genes were found, suggesting that both pathways may be involved in the susceptibility to multiple conditions affecting the oral cavity and bones. When combining patients that had concomitant dental caries, periodontitis, and periapical pathology, several markers in <italic>RHEB</italic> showed statistically significant association. Multiple conditions affecting bone and teeth (i.e., dental caries, periodontitis, periapical lesion formation, and osteoporosis) appear to share similar underlying genetic etiological factors, which allow us to hypothesize that instead of individually, they should be studied in conjunction in human populations. |
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Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases?Dental cariesEndodonticsEndoplasmic reticulum stressmTOR signalingOsteoporosisPeriodontitisThe purpose of this cohort study was to identify associations between combined oral and bone disease phenotypes and genes present in cell regulatory pathways. The studied pathways play important roles in cellular growth, proliferation, differentiation, and homeostasis. DNA samples extracted from whole saliva of 3,912 individuals were genotyped and these data analyzed according to dental caries experience, periapical lesions, periodontitis, osteoporosis, or temporomandibular joint discomfort. Samples were obtained from the Dental Registry and DNA Repository project at the University of Pittsburgh. Twenty-seven polymorphisms in eight genes related to mTOR or endoplasmic reticulum stress pathways were selected for genotyping. Allele frequencies and Hardy-Weinberg equilibrium were calculated. Analyses were performed comparing genotypes between affected and unaffected individuals for each phenotype, as well as for the associated phenotypes combined. For all analyses, we used the software PLINK with an alpha of 0.002. Borderline associations with multiple variants of several genes were found, suggesting that both pathways may be involved in the susceptibility to multiple conditions affecting the oral cavity and bones. When combining patients that had concomitant dental caries, periodontitis, and periapical pathology, several markers in <italic>RHEB</italic> showed statistically significant association. Multiple conditions affecting bone and teeth (i.e., dental caries, periodontitis, periapical lesion formation, and osteoporosis) appear to share similar underlying genetic etiological factors, which allow us to hypothesize that instead of individually, they should be studied in conjunction in human populations.Department of Oral Biology University of PittsburghGraduate Program of Health Sciences PUC-ParanáDepartment of Oral Surgery Positivo UniversityDepartment of Endodontics University of Texas Health Sciences Center at HoustonDepartment of Pediatric Dentistry UNESPDepartment of Endodontics Texas AandM College of DentistryDepartment of Pediatric Dentistry UNESPUniversity of PittsburghPUC-ParanáPositivo UniversityUniversity of Texas Health Sciences Center at HoustonUniversidade Estadual Paulista (Unesp)Texas AandM College of DentistryBezamat, MarianaDeeley, KathleenKhaliq, ShahryarLetra, AriadneScariot, RafaelaSilva, Renato M.Weber, Megan L.Bussaneli, Diego G. [UNESP]Trevilatto, Paula C.Almarza, Alejandro J.Ouyang, HongjiaoVieira, Alexandre R.2019-10-06T15:18:49Z2019-10-06T15:18:49Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article235-241http://dx.doi.org/10.1159/000492675Caries Research, v. 53, n. 3, p. 235-241, 2019.1421-976X0008-6568http://hdl.handle.net/11449/18688710.1159/0004926752-s2.0-85053776668Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCaries Researchinfo:eu-repo/semantics/openAccess2021-10-23T20:19:26Zoai:repositorio.unesp.br:11449/186887Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:19:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
title |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
spellingShingle |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? Bezamat, Mariana Dental caries Endodontics Endoplasmic reticulum stress mTOR signaling Osteoporosis Periodontitis |
title_short |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
title_full |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
title_fullStr |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
title_full_unstemmed |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
title_sort |
Are mTOR and Endoplasmic Reticulum Stress Pathway Genes Associated with Oral and Bone Diseases? |
author |
Bezamat, Mariana |
author_facet |
Bezamat, Mariana Deeley, Kathleen Khaliq, Shahryar Letra, Ariadne Scariot, Rafaela Silva, Renato M. Weber, Megan L. Bussaneli, Diego G. [UNESP] Trevilatto, Paula C. Almarza, Alejandro J. Ouyang, Hongjiao Vieira, Alexandre R. |
author_role |
author |
author2 |
Deeley, Kathleen Khaliq, Shahryar Letra, Ariadne Scariot, Rafaela Silva, Renato M. Weber, Megan L. Bussaneli, Diego G. [UNESP] Trevilatto, Paula C. Almarza, Alejandro J. Ouyang, Hongjiao Vieira, Alexandre R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Pittsburgh PUC-Paraná Positivo University University of Texas Health Sciences Center at Houston Universidade Estadual Paulista (Unesp) Texas AandM College of Dentistry |
dc.contributor.author.fl_str_mv |
Bezamat, Mariana Deeley, Kathleen Khaliq, Shahryar Letra, Ariadne Scariot, Rafaela Silva, Renato M. Weber, Megan L. Bussaneli, Diego G. [UNESP] Trevilatto, Paula C. Almarza, Alejandro J. Ouyang, Hongjiao Vieira, Alexandre R. |
dc.subject.por.fl_str_mv |
Dental caries Endodontics Endoplasmic reticulum stress mTOR signaling Osteoporosis Periodontitis |
topic |
Dental caries Endodontics Endoplasmic reticulum stress mTOR signaling Osteoporosis Periodontitis |
description |
The purpose of this cohort study was to identify associations between combined oral and bone disease phenotypes and genes present in cell regulatory pathways. The studied pathways play important roles in cellular growth, proliferation, differentiation, and homeostasis. DNA samples extracted from whole saliva of 3,912 individuals were genotyped and these data analyzed according to dental caries experience, periapical lesions, periodontitis, osteoporosis, or temporomandibular joint discomfort. Samples were obtained from the Dental Registry and DNA Repository project at the University of Pittsburgh. Twenty-seven polymorphisms in eight genes related to mTOR or endoplasmic reticulum stress pathways were selected for genotyping. Allele frequencies and Hardy-Weinberg equilibrium were calculated. Analyses were performed comparing genotypes between affected and unaffected individuals for each phenotype, as well as for the associated phenotypes combined. For all analyses, we used the software PLINK with an alpha of 0.002. Borderline associations with multiple variants of several genes were found, suggesting that both pathways may be involved in the susceptibility to multiple conditions affecting the oral cavity and bones. When combining patients that had concomitant dental caries, periodontitis, and periapical pathology, several markers in <italic>RHEB</italic> showed statistically significant association. Multiple conditions affecting bone and teeth (i.e., dental caries, periodontitis, periapical lesion formation, and osteoporosis) appear to share similar underlying genetic etiological factors, which allow us to hypothesize that instead of individually, they should be studied in conjunction in human populations. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:18:49Z 2019-10-06T15:18:49Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000492675 Caries Research, v. 53, n. 3, p. 235-241, 2019. 1421-976X 0008-6568 http://hdl.handle.net/11449/186887 10.1159/000492675 2-s2.0-85053776668 |
url |
http://dx.doi.org/10.1159/000492675 http://hdl.handle.net/11449/186887 |
identifier_str_mv |
Caries Research, v. 53, n. 3, p. 235-241, 2019. 1421-976X 0008-6568 10.1159/000492675 2-s2.0-85053776668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Caries Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
235-241 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964688925065216 |