Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/2013.July.8.9 http://hdl.handle.net/11449/75931 |
Resumo: | The incidence of colorectal cancer is growing worldwide. The characterization of compounds present in the human diet that can prevent the occurrence of colorectal tumors is vital. The oligosaccharide inulin is such a compound. The aim of this study was to evaluate the antigenotoxic, antimutagenic and anticarcinogenic effects of inulin in vivo. Our study is based on 3 assays that are widely used to evaluate chemoprevention (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, post-treatment, and pre + continuous). Experiments were carried out in Swiss male mice of reproductive age. In order to induce DNA damage, we used the pro-carcinogenic agent 1,2-dimethylhydrazine. Inulin was administered orally at a concentration of 50 mg/kg body weight following the protocols mentioned above. Inulin was not administered to the control groups. Our data from the micronucleus assay reveal antimutagenic effects of inulin in all protocols. The percentage of inulin-induced damage reduction ranged from 47.25 to 141.75% across protocols. These data suggest that inulin could act through desmutagenic and bio-antimutagenic mechanisms. The anticarcinogenic activity (aberrant crypt focus assay) of inulin was observed in all protocols and the percentages of damage reduction ranged from 55.78 to 87.56% across protocols. Further tests, including human trials, will be necessary before this functional food can be proven to be effective in the prevention and treatment of colon cancer. © FUNPEC-RP. |
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Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivoChemopreventionFiberInulin1,2 dimethylhydrazineDNAinulinaberrant crypt focus assayanalytic methodanimal cellanimal experimentantigenotoxic activityantimutagenic activityantineoplastic activitybody weightcomet assayconcentration responseDNA damagedrug activitydrug determinationin vivo studymalemicronucleus testmousenonhumanThe incidence of colorectal cancer is growing worldwide. The characterization of compounds present in the human diet that can prevent the occurrence of colorectal tumors is vital. The oligosaccharide inulin is such a compound. The aim of this study was to evaluate the antigenotoxic, antimutagenic and anticarcinogenic effects of inulin in vivo. Our study is based on 3 assays that are widely used to evaluate chemoprevention (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, post-treatment, and pre + continuous). Experiments were carried out in Swiss male mice of reproductive age. In order to induce DNA damage, we used the pro-carcinogenic agent 1,2-dimethylhydrazine. Inulin was administered orally at a concentration of 50 mg/kg body weight following the protocols mentioned above. Inulin was not administered to the control groups. Our data from the micronucleus assay reveal antimutagenic effects of inulin in all protocols. The percentage of inulin-induced damage reduction ranged from 47.25 to 141.75% across protocols. These data suggest that inulin could act through desmutagenic and bio-antimutagenic mechanisms. The anticarcinogenic activity (aberrant crypt focus assay) of inulin was observed in all protocols and the percentages of damage reduction ranged from 55.78 to 87.56% across protocols. Further tests, including human trials, will be necessary before this functional food can be proven to be effective in the prevention and treatment of colon cancer. © FUNPEC-RP.Centro de Estudos em Nutrição e Genética Toxicológica Centro Universitário Filadélfia, Londrina, PRInstituto de Biociências de Rio Claro Universidade Estadual Paulista, Rio Claro, SPCentro de Estudos em Celulas Tronco, Terapia Celular e Genética Toxicologica - CeTroGen Núcleo de Hospital Universitário Universidade Federal de Mato Grosso do Sul, Campo Grande, MSCentro de Ciências Biológicas e da Saúde Universidade Federal de Mato Grosso do Sul, Campo Grande, MSDepartamento de Biologia Centro de Ciências Biológicas Universidade Estadual de Londrina, Londrina, PRCentro de Ciências da Saúde Departamento de Ciências Farmacêuticas Universidade Estadual de Londrina, Londrina, PRUniversidade Federal de Mato Grosso do Sul, Campo Grande, MSInstituto de Biociências de Rio Claro Universidade Estadual Paulista, Rio Claro, SPCentro Universitário FiladélfiaUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual de Londrina (UEL)Mauro, M. O. [UNESP]Monreal, M. T F DSilva, M. T P [UNESP]Pesarini, J. R. [UNESP]Mantovani, M. S.Ribeiro, L. R. [UNESP]Dichi, J. B.Carreira, C. M.Oliveira, R. J.2014-05-27T11:29:55Z2014-05-27T11:29:55Z2013-07-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2281-2293application/pdfhttp://dx.doi.org/10.4238/2013.July.8.9Genetics and Molecular Research, v. 12, n. 3, p. 2281-2293, 2013.1676-5680http://hdl.handle.net/11449/7593110.4238/2013.July.8.9WOS:0003317174000122-s2.0-848801271162-s2.0-84880127116.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-07T06:13:24Zoai:repositorio.unesp.br:11449/75931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-07T06:13:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
title |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
spellingShingle |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo Mauro, M. O. [UNESP] Chemoprevention Fiber Inulin 1,2 dimethylhydrazine DNA inulin aberrant crypt focus assay analytic method animal cell animal experiment antigenotoxic activity antimutagenic activity antineoplastic activity body weight comet assay concentration response DNA damage drug activity drug determination in vivo study male micronucleus test mouse nonhuman |
title_short |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
title_full |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
title_fullStr |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
title_full_unstemmed |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
title_sort |
Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo |
author |
Mauro, M. O. [UNESP] |
author_facet |
Mauro, M. O. [UNESP] Monreal, M. T F D Silva, M. T P [UNESP] Pesarini, J. R. [UNESP] Mantovani, M. S. Ribeiro, L. R. [UNESP] Dichi, J. B. Carreira, C. M. Oliveira, R. J. |
author_role |
author |
author2 |
Monreal, M. T F D Silva, M. T P [UNESP] Pesarini, J. R. [UNESP] Mantovani, M. S. Ribeiro, L. R. [UNESP] Dichi, J. B. Carreira, C. M. Oliveira, R. J. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro Universitário Filadélfia Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual de Londrina (UEL) |
dc.contributor.author.fl_str_mv |
Mauro, M. O. [UNESP] Monreal, M. T F D Silva, M. T P [UNESP] Pesarini, J. R. [UNESP] Mantovani, M. S. Ribeiro, L. R. [UNESP] Dichi, J. B. Carreira, C. M. Oliveira, R. J. |
dc.subject.por.fl_str_mv |
Chemoprevention Fiber Inulin 1,2 dimethylhydrazine DNA inulin aberrant crypt focus assay analytic method animal cell animal experiment antigenotoxic activity antimutagenic activity antineoplastic activity body weight comet assay concentration response DNA damage drug activity drug determination in vivo study male micronucleus test mouse nonhuman |
topic |
Chemoprevention Fiber Inulin 1,2 dimethylhydrazine DNA inulin aberrant crypt focus assay analytic method animal cell animal experiment antigenotoxic activity antimutagenic activity antineoplastic activity body weight comet assay concentration response DNA damage drug activity drug determination in vivo study male micronucleus test mouse nonhuman |
description |
The incidence of colorectal cancer is growing worldwide. The characterization of compounds present in the human diet that can prevent the occurrence of colorectal tumors is vital. The oligosaccharide inulin is such a compound. The aim of this study was to evaluate the antigenotoxic, antimutagenic and anticarcinogenic effects of inulin in vivo. Our study is based on 3 assays that are widely used to evaluate chemoprevention (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, post-treatment, and pre + continuous). Experiments were carried out in Swiss male mice of reproductive age. In order to induce DNA damage, we used the pro-carcinogenic agent 1,2-dimethylhydrazine. Inulin was administered orally at a concentration of 50 mg/kg body weight following the protocols mentioned above. Inulin was not administered to the control groups. Our data from the micronucleus assay reveal antimutagenic effects of inulin in all protocols. The percentage of inulin-induced damage reduction ranged from 47.25 to 141.75% across protocols. These data suggest that inulin could act through desmutagenic and bio-antimutagenic mechanisms. The anticarcinogenic activity (aberrant crypt focus assay) of inulin was observed in all protocols and the percentages of damage reduction ranged from 55.78 to 87.56% across protocols. Further tests, including human trials, will be necessary before this functional food can be proven to be effective in the prevention and treatment of colon cancer. © FUNPEC-RP. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-08 2014-05-27T11:29:55Z 2014-05-27T11:29:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/2013.July.8.9 Genetics and Molecular Research, v. 12, n. 3, p. 2281-2293, 2013. 1676-5680 http://hdl.handle.net/11449/75931 10.4238/2013.July.8.9 WOS:000331717400012 2-s2.0-84880127116 2-s2.0-84880127116.pdf |
url |
http://dx.doi.org/10.4238/2013.July.8.9 http://hdl.handle.net/11449/75931 |
identifier_str_mv |
Genetics and Molecular Research, v. 12, n. 3, p. 2281-2293, 2013. 1676-5680 10.4238/2013.July.8.9 WOS:000331717400012 2-s2.0-84880127116 2-s2.0-84880127116.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2281-2293 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789925680087040 |