High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity
Main Author: | |
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Publication Date: | 2009 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3390/molecules14114661 http://hdl.handle.net/11449/225723 |
Summary: | Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines. |
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High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activityEriocaulaceaeFlavonoidsLiquid chromatographyQuantificationQuantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.UNESP-Univ Estadual Paulista Instituto de Química Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São PauloUEMS Course of Chemistry Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do SulUNESP-Univ Estadual Paulista Instituto de Química Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São PauloUniversidade Estadual Paulista (UNESP)Universidade Estadual de Mato Grosso do Sul (UEMS)Da Silva, Marcelo Aparecido [UNESP]Cardoso, Claudia Andréa LimaVilegas, Wagner [UNESP]Dos Santos, Lourdes Campaner [UNESP]2022-04-28T20:59:05Z2022-04-28T20:59:05Z2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4644-4654http://dx.doi.org/10.3390/molecules14114661Molecules, v. 14, n. 11, p. 4644-4654, 2009.1420-3049http://hdl.handle.net/11449/22572310.3390/molecules141146612-s2.0-73349097549Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2022-04-28T20:59:05Zoai:repositorio.unesp.br:11449/225723Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:59:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
title |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
spellingShingle |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity Da Silva, Marcelo Aparecido [UNESP] Eriocaulaceae Flavonoids Liquid chromatography Quantification |
title_short |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
title_full |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
title_fullStr |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
title_full_unstemmed |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
title_sort |
High-performance liquid chromatographic quantification of flavonoids in Eriocaulaceae species and their antimicrobial activity |
author |
Da Silva, Marcelo Aparecido [UNESP] |
author_facet |
Da Silva, Marcelo Aparecido [UNESP] Cardoso, Claudia Andréa Lima Vilegas, Wagner [UNESP] Dos Santos, Lourdes Campaner [UNESP] |
author_role |
author |
author2 |
Cardoso, Claudia Andréa Lima Vilegas, Wagner [UNESP] Dos Santos, Lourdes Campaner [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Mato Grosso do Sul (UEMS) |
dc.contributor.author.fl_str_mv |
Da Silva, Marcelo Aparecido [UNESP] Cardoso, Claudia Andréa Lima Vilegas, Wagner [UNESP] Dos Santos, Lourdes Campaner [UNESP] |
dc.subject.por.fl_str_mv |
Eriocaulaceae Flavonoids Liquid chromatography Quantification |
topic |
Eriocaulaceae Flavonoids Liquid chromatography Quantification |
description |
Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 2022-04-28T20:59:05Z 2022-04-28T20:59:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules14114661 Molecules, v. 14, n. 11, p. 4644-4654, 2009. 1420-3049 http://hdl.handle.net/11449/225723 10.3390/molecules14114661 2-s2.0-73349097549 |
url |
http://dx.doi.org/10.3390/molecules14114661 http://hdl.handle.net/11449/225723 |
identifier_str_mv |
Molecules, v. 14, n. 11, p. 4644-4654, 2009. 1420-3049 10.3390/molecules14114661 2-s2.0-73349097549 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4644-4654 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789260696256512 |