HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5935/0100-4042.20140193 http://hdl.handle.net/11449/114543 |
Resumo: | This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid). |
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HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activityEriocaulaceaeTonina fluviatilisflavonoidsHPLC-DADantiradical activityThis article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Instituto de Química Departamento de Química OrgânicaUniversidade Estadual Paulista Departamento de QuímicaUniversidade Estadual PaulistaUniversidade Santa CecíliaUniversidade Federal de Mato Grosso Instituto de Ciências Naturais, Humanas e SociaisUniversidade Estadual Paulista Instituto de Química Departamento de Química OrgânicaUniversidade Estadual Paulista Departamento de QuímicaUniversidade Estadual PaulistaSociedade Brasileira de QuímicaUniversidade Estadual Paulista (Unesp)Universidade Santa CecíliaUniversidade Federal de Mato Grosso Instituto de Ciências Naturais, Humanas e SociaisAmorim, Marcelo R. De [UNESP]Rinaldo, Daniel [UNESP]Amaral, Fabiano P. Do [UNESP]Vilegas, Wagner [UNESP]Magenta, Mara A. G.Vieira Jr, Gerardo M.Santos, Lourdes Campaner dos [UNESP]2015-02-02T12:39:38Z2015-02-02T12:39:38Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1122-1127application/pdfhttp://dx.doi.org/10.5935/0100-4042.20140193Química Nova. Sociedade Brasileira de Química, v. 37, n. 7, p. 1122-1127, 2014.0100-4042http://hdl.handle.net/11449/11454310.5935/0100-4042.20140193S0100-40422014000700003S0100-40422014000700003.pdf35382536406029770000-0003-3032-2556SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengQuímica Nova0.6460,255info:eu-repo/semantics/openAccess2023-11-03T06:14:02Zoai:repositorio.unesp.br:11449/114543Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-03T06:14:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
title |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
spellingShingle |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity Amorim, Marcelo R. De [UNESP] Eriocaulaceae Tonina fluviatilis flavonoids HPLC-DAD antiradical activity |
title_short |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
title_full |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
title_fullStr |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
title_full_unstemmed |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
title_sort |
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity |
author |
Amorim, Marcelo R. De [UNESP] |
author_facet |
Amorim, Marcelo R. De [UNESP] Rinaldo, Daniel [UNESP] Amaral, Fabiano P. Do [UNESP] Vilegas, Wagner [UNESP] Magenta, Mara A. G. Vieira Jr, Gerardo M. Santos, Lourdes Campaner dos [UNESP] |
author_role |
author |
author2 |
Rinaldo, Daniel [UNESP] Amaral, Fabiano P. Do [UNESP] Vilegas, Wagner [UNESP] Magenta, Mara A. G. Vieira Jr, Gerardo M. Santos, Lourdes Campaner dos [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Santa Cecília Universidade Federal de Mato Grosso Instituto de Ciências Naturais, Humanas e Sociais |
dc.contributor.author.fl_str_mv |
Amorim, Marcelo R. De [UNESP] Rinaldo, Daniel [UNESP] Amaral, Fabiano P. Do [UNESP] Vilegas, Wagner [UNESP] Magenta, Mara A. G. Vieira Jr, Gerardo M. Santos, Lourdes Campaner dos [UNESP] |
dc.subject.por.fl_str_mv |
Eriocaulaceae Tonina fluviatilis flavonoids HPLC-DAD antiradical activity |
topic |
Eriocaulaceae Tonina fluviatilis flavonoids HPLC-DAD antiradical activity |
description |
This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid). |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-02-02T12:39:38Z 2015-02-02T12:39:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5935/0100-4042.20140193 Química Nova. Sociedade Brasileira de Química, v. 37, n. 7, p. 1122-1127, 2014. 0100-4042 http://hdl.handle.net/11449/114543 10.5935/0100-4042.20140193 S0100-40422014000700003 S0100-40422014000700003.pdf 3538253640602977 0000-0003-3032-2556 |
url |
http://dx.doi.org/10.5935/0100-4042.20140193 http://hdl.handle.net/11449/114543 |
identifier_str_mv |
Química Nova. Sociedade Brasileira de Química, v. 37, n. 7, p. 1122-1127, 2014. 0100-4042 10.5935/0100-4042.20140193 S0100-40422014000700003 S0100-40422014000700003.pdf 3538253640602977 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Química Nova 0.646 0,255 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1122-1127 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964809255452672 |