High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules14114644 http://hdl.handle.net/11449/26159 |
Resumo: | Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines. |
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High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial ActivityEriocaulaceaeliquid chromatographyquantificationflavonoidsQuantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 São Paulo, BrazilMato Grosso Sul State Univ, Course Chem, UEMS, BR-79804970 Dourados, Mato Grosso Su, BrazilUNESP Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 São Paulo, BrazilMolecular Diversity Preservation International-mdpiUniversidade Estadual Paulista (Unesp)Mato Grosso Sul State Univda Silva, Marcelo Aparecido [UNESP]Lima Cardoso, Claudia AndreaVilegas, Wagner [UNESP]Santos, Lourdes Campaner dos [UNESP]2014-05-20T14:20:28Z2014-05-20T14:20:28Z2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4644-4654application/pdfhttp://dx.doi.org/10.3390/molecules14114644Molecules. Basel: Molecular Diversity Preservation International-mdpi, v. 14, n. 11, p. 4644-4654, 2009.1420-3049http://hdl.handle.net/11449/2615910.3390/molecules14114644WOS:000272190500028WOS000272190500028.pdf35382536406029770000-0003-3032-2556Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules3.0980,855info:eu-repo/semantics/openAccess2023-10-26T06:05:39Zoai:repositorio.unesp.br:11449/26159Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:05:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
title |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
spellingShingle |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity da Silva, Marcelo Aparecido [UNESP] Eriocaulaceae liquid chromatography quantification flavonoids |
title_short |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
title_full |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
title_fullStr |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
title_full_unstemmed |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
title_sort |
High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity |
author |
da Silva, Marcelo Aparecido [UNESP] |
author_facet |
da Silva, Marcelo Aparecido [UNESP] Lima Cardoso, Claudia Andrea Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] |
author_role |
author |
author2 |
Lima Cardoso, Claudia Andrea Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Mato Grosso Sul State Univ |
dc.contributor.author.fl_str_mv |
da Silva, Marcelo Aparecido [UNESP] Lima Cardoso, Claudia Andrea Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] |
dc.subject.por.fl_str_mv |
Eriocaulaceae liquid chromatography quantification flavonoids |
topic |
Eriocaulaceae liquid chromatography quantification flavonoids |
description |
Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 2014-05-20T14:20:28Z 2014-05-20T14:20:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules14114644 Molecules. Basel: Molecular Diversity Preservation International-mdpi, v. 14, n. 11, p. 4644-4654, 2009. 1420-3049 http://hdl.handle.net/11449/26159 10.3390/molecules14114644 WOS:000272190500028 WOS000272190500028.pdf 3538253640602977 0000-0003-3032-2556 |
url |
http://dx.doi.org/10.3390/molecules14114644 http://hdl.handle.net/11449/26159 |
identifier_str_mv |
Molecules. Basel: Molecular Diversity Preservation International-mdpi, v. 14, n. 11, p. 4644-4654, 2009. 1420-3049 10.3390/molecules14114644 WOS:000272190500028 WOS000272190500028.pdf 3538253640602977 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 3.098 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4644-4654 application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International-mdpi |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International-mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789513062285312 |