Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-020-06918-7 http://hdl.handle.net/11449/206727 |
Resumo: | The present study describes Henneguya tietensis n. sp. parasitizing Prochilodus lineatus (Valenciennes, 1837) from Brazil, through morphological and molecular analysis. Myxospores were found in the gill tissue of P. lineatus with a prevalence of 10%. The myxospores consisted of two elongate, elliptical shell valves each with a long, tapering caudal appendage. The morphology was consistent with Henneguya and the myxospore measured as follows (mean ± SD): total length 55.5 ± 2.1 μm, body length 16.2 ± 1.1 μm, body width 5.5 ± 0.1 μm, caudal appendages length 39.0 ± 2.0 μm. The polar capsules were 7.3 ± 0.2 μm long, 1.7 ± 0.2 μm wide and contained a polar filament coiled 11 to 13 turns. Histological analysis showed the plasmodia developing in the middle region of each gill filament and caused a focally extensive distension by forming a space-occupying mass within the gill filament. The ultrastructural analysis allowed the observation of the presence of mature myxospores throughout the plasmodium. Phylogenetic analysis with the SSU rDNA gene showed Henneguya tietensis n. sp. as a sister species of the subclade formed by Henneguya piaractus and Henneguya brachypomus that infect fishes of the genus Piaractus. The genetically closest species was H. piaractus, which showed a similarity of 82.4%. Using molecular and morphological characterization, the myxozoan parasite described herein represents a new species of the genus Henneguya. |
id |
UNSP_2f12fbd97958f84a6c62a8cd87aeff03 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/206727 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from BrazilCharaciformesHenneguyaMyxobolidaePhylogenyTietê RiverThe present study describes Henneguya tietensis n. sp. parasitizing Prochilodus lineatus (Valenciennes, 1837) from Brazil, through morphological and molecular analysis. Myxospores were found in the gill tissue of P. lineatus with a prevalence of 10%. The myxospores consisted of two elongate, elliptical shell valves each with a long, tapering caudal appendage. The morphology was consistent with Henneguya and the myxospore measured as follows (mean ± SD): total length 55.5 ± 2.1 μm, body length 16.2 ± 1.1 μm, body width 5.5 ± 0.1 μm, caudal appendages length 39.0 ± 2.0 μm. The polar capsules were 7.3 ± 0.2 μm long, 1.7 ± 0.2 μm wide and contained a polar filament coiled 11 to 13 turns. Histological analysis showed the plasmodia developing in the middle region of each gill filament and caused a focally extensive distension by forming a space-occupying mass within the gill filament. The ultrastructural analysis allowed the observation of the presence of mature myxospores throughout the plasmodium. Phylogenetic analysis with the SSU rDNA gene showed Henneguya tietensis n. sp. as a sister species of the subclade formed by Henneguya piaractus and Henneguya brachypomus that infect fishes of the genus Piaractus. The genetically closest species was H. piaractus, which showed a similarity of 82.4%. Using molecular and morphological characterization, the myxozoan parasite described herein represents a new species of the genus Henneguya.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departament of Parasitology Universidade Estadual Paulista “Júlio de Mesquita Filho” – Unesp, R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Distrito de Rubião JuniorFaculdade de Ciências e CIIMAR Universidade do PortoDocente do Programa de Pós-graduação em Análise de Sistemas Ambientais Centro Universitário CESMACDepartament of Parasitology Universidade Estadual Paulista “Júlio de Mesquita Filho” – Unesp, R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Distrito de Rubião JuniorFAPESP: 2014/12862-0FAPESP: 2015/24901-2FAPESP: 2019/07708-5Universidade Estadual Paulista (Unesp)Universidade do PortoCentro Universitário CESMACVieira, Diego Henrique Mirandola Dias [UNESP]Rangel, Luís FilipeTagliavini, Vinicius Panciera [UNESP]Abdallah, Vanessa DoroSantos, Maria Joãode Azevedo, Rodney Kozlowiski2021-06-25T10:37:12Z2021-06-25T10:37:12Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-36http://dx.doi.org/10.1007/s00436-020-06918-7Parasitology Research, v. 120, n. 1, p. 27-36, 2021.1432-19550932-0113http://hdl.handle.net/11449/20672710.1007/s00436-020-06918-72-s2.0-85093927688Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2021-10-23T12:39:35Zoai:repositorio.unesp.br:11449/206727Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:39:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
title |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
spellingShingle |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil Vieira, Diego Henrique Mirandola Dias [UNESP] Characiformes Henneguya Myxobolidae Phylogeny Tietê River |
title_short |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
title_full |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
title_fullStr |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
title_full_unstemmed |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
title_sort |
Morphological and molecular analysis of Henneguya tietensis n. sp. (Cnidaria: Myxosporea), parasitizing the gill filaments of Prochilodus lineatus (Valenciennes, 1837) from Brazil |
author |
Vieira, Diego Henrique Mirandola Dias [UNESP] |
author_facet |
Vieira, Diego Henrique Mirandola Dias [UNESP] Rangel, Luís Filipe Tagliavini, Vinicius Panciera [UNESP] Abdallah, Vanessa Doro Santos, Maria João de Azevedo, Rodney Kozlowiski |
author_role |
author |
author2 |
Rangel, Luís Filipe Tagliavini, Vinicius Panciera [UNESP] Abdallah, Vanessa Doro Santos, Maria João de Azevedo, Rodney Kozlowiski |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade do Porto Centro Universitário CESMAC |
dc.contributor.author.fl_str_mv |
Vieira, Diego Henrique Mirandola Dias [UNESP] Rangel, Luís Filipe Tagliavini, Vinicius Panciera [UNESP] Abdallah, Vanessa Doro Santos, Maria João de Azevedo, Rodney Kozlowiski |
dc.subject.por.fl_str_mv |
Characiformes Henneguya Myxobolidae Phylogeny Tietê River |
topic |
Characiformes Henneguya Myxobolidae Phylogeny Tietê River |
description |
The present study describes Henneguya tietensis n. sp. parasitizing Prochilodus lineatus (Valenciennes, 1837) from Brazil, through morphological and molecular analysis. Myxospores were found in the gill tissue of P. lineatus with a prevalence of 10%. The myxospores consisted of two elongate, elliptical shell valves each with a long, tapering caudal appendage. The morphology was consistent with Henneguya and the myxospore measured as follows (mean ± SD): total length 55.5 ± 2.1 μm, body length 16.2 ± 1.1 μm, body width 5.5 ± 0.1 μm, caudal appendages length 39.0 ± 2.0 μm. The polar capsules were 7.3 ± 0.2 μm long, 1.7 ± 0.2 μm wide and contained a polar filament coiled 11 to 13 turns. Histological analysis showed the plasmodia developing in the middle region of each gill filament and caused a focally extensive distension by forming a space-occupying mass within the gill filament. The ultrastructural analysis allowed the observation of the presence of mature myxospores throughout the plasmodium. Phylogenetic analysis with the SSU rDNA gene showed Henneguya tietensis n. sp. as a sister species of the subclade formed by Henneguya piaractus and Henneguya brachypomus that infect fishes of the genus Piaractus. The genetically closest species was H. piaractus, which showed a similarity of 82.4%. Using molecular and morphological characterization, the myxozoan parasite described herein represents a new species of the genus Henneguya. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:37:12Z 2021-06-25T10:37:12Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-020-06918-7 Parasitology Research, v. 120, n. 1, p. 27-36, 2021. 1432-1955 0932-0113 http://hdl.handle.net/11449/206727 10.1007/s00436-020-06918-7 2-s2.0-85093927688 |
url |
http://dx.doi.org/10.1007/s00436-020-06918-7 http://hdl.handle.net/11449/206727 |
identifier_str_mv |
Parasitology Research, v. 120, n. 1, p. 27-36, 2021. 1432-1955 0932-0113 10.1007/s00436-020-06918-7 2-s2.0-85093927688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-36 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962365220716544 |