Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/21505594.2018.1518087 http://hdl.handle.net/11449/186919 |
Resumo: | Laboratory investigations of the pathogenesis of Pseudogymnoascus destructans, the fungal causal agent of bat White Nose Syndrome (WNS), presents unique challenges due to its growth requirements (4°-15°C) and a lack of infectivity in the current disease models. Pseudogymnoascus pannorum is the nearest fungal relative of P. destructans with wider psychrophilic - physiological growth range, and ability to cause rare skin infections in humans. Our broad objectives are to create the molecular toolkit for comparative study of P. destructans and P. pannorum pathogenesis. Towards these goals, we report the successful development of an invertebrate model in the greater wax moth Galleria mellonella. Both P. destructans and P. pannorum caused fatal disease in G. mellonella and elicited immune responses and histopathological changes consistent with the experimental disease. |
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Repositório Institucional da UNESP |
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Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorumGalleria mellonellaHistopathologyInfection modelInvertebrate hostPseudogymnoascus destructansPseudogymnoascus pannorumSurvival curveLaboratory investigations of the pathogenesis of Pseudogymnoascus destructans, the fungal causal agent of bat White Nose Syndrome (WNS), presents unique challenges due to its growth requirements (4°-15°C) and a lack of infectivity in the current disease models. Pseudogymnoascus pannorum is the nearest fungal relative of P. destructans with wider psychrophilic - physiological growth range, and ability to cause rare skin infections in humans. Our broad objectives are to create the molecular toolkit for comparative study of P. destructans and P. pannorum pathogenesis. Towards these goals, we report the successful development of an invertebrate model in the greater wax moth Galleria mellonella. Both P. destructans and P. pannorum caused fatal disease in G. mellonella and elicited immune responses and histopathological changes consistent with the experimental disease.National Science FoundationDivision of Infectious Diseases Rhode Island Hospital Warren Alpert Medical School Brown UniversityMycology Laboratory Division of Infectious Diseases Wadsworth Center New York State Department of HealthDepartment of Biomedical Sciences School of Public Health University of AlbanyDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual PaulistaDivision of Infectious Diseases Wadsworth Center New York State Department of HealthDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual PaulistaNational Science Foundation: 1203528Brown UniversityNew York State Department of HealthUniversity of AlbanyUniversidade Estadual Paulista (Unesp)Fuchs, Beth BurgwynChaturvedi, SudhaRossoni, Rodnei Dennis [UNESP]De Barros, Patricia P. [UNESP]Torres-Velez, FernandoMylonakis, EleftheriosChaturvedi, Vishnu2019-10-06T15:19:50Z2019-10-06T15:19:50Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1539-1547http://dx.doi.org/10.1080/21505594.2018.1518087Virulence, v. 9, n. 1, p. 1539-1547, 2018.2150-56082150-5594http://hdl.handle.net/11449/18691910.1080/21505594.2018.15180872-s2.0-85054423725Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirulenceinfo:eu-repo/semantics/openAccess2021-10-23T00:57:27Zoai:repositorio.unesp.br:11449/186919Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T00:57:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
title |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
spellingShingle |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum Fuchs, Beth Burgwyn Galleria mellonella Histopathology Infection model Invertebrate host Pseudogymnoascus destructans Pseudogymnoascus pannorum Survival curve |
title_short |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
title_full |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
title_fullStr |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
title_full_unstemmed |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
title_sort |
Galleria mellonella experimental model for bat fungal pathogen Pseudogymnoascus destructans and human fungal pathogen Pseudogymnoascus pannorum |
author |
Fuchs, Beth Burgwyn |
author_facet |
Fuchs, Beth Burgwyn Chaturvedi, Sudha Rossoni, Rodnei Dennis [UNESP] De Barros, Patricia P. [UNESP] Torres-Velez, Fernando Mylonakis, Eleftherios Chaturvedi, Vishnu |
author_role |
author |
author2 |
Chaturvedi, Sudha Rossoni, Rodnei Dennis [UNESP] De Barros, Patricia P. [UNESP] Torres-Velez, Fernando Mylonakis, Eleftherios Chaturvedi, Vishnu |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Brown University New York State Department of Health University of Albany Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fuchs, Beth Burgwyn Chaturvedi, Sudha Rossoni, Rodnei Dennis [UNESP] De Barros, Patricia P. [UNESP] Torres-Velez, Fernando Mylonakis, Eleftherios Chaturvedi, Vishnu |
dc.subject.por.fl_str_mv |
Galleria mellonella Histopathology Infection model Invertebrate host Pseudogymnoascus destructans Pseudogymnoascus pannorum Survival curve |
topic |
Galleria mellonella Histopathology Infection model Invertebrate host Pseudogymnoascus destructans Pseudogymnoascus pannorum Survival curve |
description |
Laboratory investigations of the pathogenesis of Pseudogymnoascus destructans, the fungal causal agent of bat White Nose Syndrome (WNS), presents unique challenges due to its growth requirements (4°-15°C) and a lack of infectivity in the current disease models. Pseudogymnoascus pannorum is the nearest fungal relative of P. destructans with wider psychrophilic - physiological growth range, and ability to cause rare skin infections in humans. Our broad objectives are to create the molecular toolkit for comparative study of P. destructans and P. pannorum pathogenesis. Towards these goals, we report the successful development of an invertebrate model in the greater wax moth Galleria mellonella. Both P. destructans and P. pannorum caused fatal disease in G. mellonella and elicited immune responses and histopathological changes consistent with the experimental disease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2019-10-06T15:19:50Z 2019-10-06T15:19:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/21505594.2018.1518087 Virulence, v. 9, n. 1, p. 1539-1547, 2018. 2150-5608 2150-5594 http://hdl.handle.net/11449/186919 10.1080/21505594.2018.1518087 2-s2.0-85054423725 |
url |
http://dx.doi.org/10.1080/21505594.2018.1518087 http://hdl.handle.net/11449/186919 |
identifier_str_mv |
Virulence, v. 9, n. 1, p. 1539-1547, 2018. 2150-5608 2150-5594 10.1080/21505594.2018.1518087 2-s2.0-85054423725 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Virulence |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1539-1547 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789607557857280 |