Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin
Main Author: | |
---|---|
Publication Date: | 2011 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3390/molecules16010738 http://hdl.handle.net/11449/42348 |
Summary: | In this work we have characterized the action of the naringin, a flavonoid found in grapefruit and known for its various pharmacological effects, which include antioxidant, blood lipid lowering and anticancer activity, on the structure and biochemical activities of a secretory phospholipase A (sPLA2) from Crotalus durissus cascavella, an important protein involved in the releasinge of arachidonic acid in phospholipid membranes. sPLA2 was incubated with naringin (mol:mol) at 37 degrees C and a discrete reduction in the UV scanning signal and a modification of the circular dichroism spectra were observed after treatment with naringin, suggesting modifications of the secondary structure of the protein. This flavonoid was able to decrease enzymatic activity and some pharmacological effects, such as myonecrosis, platelet aggregation, and neurotoxic activity caused by sPLA2, however, the inflammatory effect was not affected by naringin. In addition, small angle X-ray scattering (SAXS) data were collected for sPLA2 and naringin-treated sPLA2 to evaluate possible modifications of the protein structure. These structural investigations have shown that sPLA2 is an elongated dimer in solution and after treatment with naringin a conformational change in the dimeric configuration was observed. Our results suggest that structural modification may be correlated with the loss of enzymatic activity and alterations in pharmacological properties. |
id |
UNSP_704ffdfc559e743e47c23ea164237476 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/42348 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringinsecretoy phospholipase A2Crotalus durissus cascavellanaringinenzymatic activity pharmacological effectssmall angle X-ray scatteringIn this work we have characterized the action of the naringin, a flavonoid found in grapefruit and known for its various pharmacological effects, which include antioxidant, blood lipid lowering and anticancer activity, on the structure and biochemical activities of a secretory phospholipase A (sPLA2) from Crotalus durissus cascavella, an important protein involved in the releasinge of arachidonic acid in phospholipid membranes. sPLA2 was incubated with naringin (mol:mol) at 37 degrees C and a discrete reduction in the UV scanning signal and a modification of the circular dichroism spectra were observed after treatment with naringin, suggesting modifications of the secondary structure of the protein. This flavonoid was able to decrease enzymatic activity and some pharmacological effects, such as myonecrosis, platelet aggregation, and neurotoxic activity caused by sPLA2, however, the inflammatory effect was not affected by naringin. In addition, small angle X-ray scattering (SAXS) data were collected for sPLA2 and naringin-treated sPLA2 to evaluate possible modifications of the protein structure. These structural investigations have shown that sPLA2 is an elongated dimer in solution and after treatment with naringin a conformational change in the dimeric configuration was observed. Our results suggest that structural modification may be correlated with the loss of enzymatic activity and alterations in pharmacological properties.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAEPEX/PRP/UNICAMPUNESP CLP, Lab Macromol Quim, São Paulo, BrazilUniv Estadual Campinas, Inst Quim, Lab Biol Estrutural & Cristalog, São Paulo, BrazilUniv Presbiteriana Mackenzie, CCBS, São Paulo, BrazilUniv Estadual Campinas, Inst Biol, Dept Bioquim, São Paulo, BrazilUNESP CLP, Lab Macromol Quim, São Paulo, BrazilMdpi AgUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Univ Presbiteriana MackenzieSantos, Marcelo L.Toyama, Daniela O.Oliveira, Simone C. B.Cotrim, Camila A.Diz-Filho, Eduardo B. S.Fagundes, Fabio H. R.Soares, Veronica C. G.Aparicio, RicardoToyama, Marcos H. [UNESP]2014-05-20T15:33:53Z2014-05-20T15:33:53Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article738-761application/pdfhttp://dx.doi.org/10.3390/molecules16010738Molecules. Basel: Mdpi Ag, v. 16, n. 1, p. 738-761, 2011.1420-3049http://hdl.handle.net/11449/4234810.3390/molecules16010738WOS:000286596400053WOS000286596400053.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules3.0980,855info:eu-repo/semantics/openAccess2023-11-28T06:15:10Zoai:repositorio.unesp.br:11449/42348Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-28T06:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
title |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
spellingShingle |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin Santos, Marcelo L. secretoy phospholipase A2 Crotalus durissus cascavella naringin enzymatic activity pharmacological effects small angle X-ray scattering |
title_short |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
title_full |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
title_fullStr |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
title_full_unstemmed |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
title_sort |
Modulation of the Pharmacological Activities of Secretory Phospholipase A2 from Crotalus durissus cascavella Induced by Naringin |
author |
Santos, Marcelo L. |
author_facet |
Santos, Marcelo L. Toyama, Daniela O. Oliveira, Simone C. B. Cotrim, Camila A. Diz-Filho, Eduardo B. S. Fagundes, Fabio H. R. Soares, Veronica C. G. Aparicio, Ricardo Toyama, Marcos H. [UNESP] |
author_role |
author |
author2 |
Toyama, Daniela O. Oliveira, Simone C. B. Cotrim, Camila A. Diz-Filho, Eduardo B. S. Fagundes, Fabio H. R. Soares, Veronica C. G. Aparicio, Ricardo Toyama, Marcos H. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Univ Presbiteriana Mackenzie |
dc.contributor.author.fl_str_mv |
Santos, Marcelo L. Toyama, Daniela O. Oliveira, Simone C. B. Cotrim, Camila A. Diz-Filho, Eduardo B. S. Fagundes, Fabio H. R. Soares, Veronica C. G. Aparicio, Ricardo Toyama, Marcos H. [UNESP] |
dc.subject.por.fl_str_mv |
secretoy phospholipase A2 Crotalus durissus cascavella naringin enzymatic activity pharmacological effects small angle X-ray scattering |
topic |
secretoy phospholipase A2 Crotalus durissus cascavella naringin enzymatic activity pharmacological effects small angle X-ray scattering |
description |
In this work we have characterized the action of the naringin, a flavonoid found in grapefruit and known for its various pharmacological effects, which include antioxidant, blood lipid lowering and anticancer activity, on the structure and biochemical activities of a secretory phospholipase A (sPLA2) from Crotalus durissus cascavella, an important protein involved in the releasinge of arachidonic acid in phospholipid membranes. sPLA2 was incubated with naringin (mol:mol) at 37 degrees C and a discrete reduction in the UV scanning signal and a modification of the circular dichroism spectra were observed after treatment with naringin, suggesting modifications of the secondary structure of the protein. This flavonoid was able to decrease enzymatic activity and some pharmacological effects, such as myonecrosis, platelet aggregation, and neurotoxic activity caused by sPLA2, however, the inflammatory effect was not affected by naringin. In addition, small angle X-ray scattering (SAXS) data were collected for sPLA2 and naringin-treated sPLA2 to evaluate possible modifications of the protein structure. These structural investigations have shown that sPLA2 is an elongated dimer in solution and after treatment with naringin a conformational change in the dimeric configuration was observed. Our results suggest that structural modification may be correlated with the loss of enzymatic activity and alterations in pharmacological properties. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2014-05-20T15:33:53Z 2014-05-20T15:33:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules16010738 Molecules. Basel: Mdpi Ag, v. 16, n. 1, p. 738-761, 2011. 1420-3049 http://hdl.handle.net/11449/42348 10.3390/molecules16010738 WOS:000286596400053 WOS000286596400053.pdf |
url |
http://dx.doi.org/10.3390/molecules16010738 http://hdl.handle.net/11449/42348 |
identifier_str_mv |
Molecules. Basel: Mdpi Ag, v. 16, n. 1, p. 738-761, 2011. 1420-3049 10.3390/molecules16010738 WOS:000286596400053 WOS000286596400053.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 3.098 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
738-761 application/pdf |
dc.publisher.none.fl_str_mv |
Mdpi Ag |
publisher.none.fl_str_mv |
Mdpi Ag |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789842608750592 |