Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos

• Autor(a) principal: Cargnin-Carvalho, Anderson
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Universitário da Ânima (RUNA)
• Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3168
• topic: Frutose; Hipotálamo; Gordura; Estresse oxidativo

Introduction: Urbanization has been dictating inadequate eating habits, with excessive consumption of sweetened drinks and high availability of fructose. From the different metabolic pathways involved in its excessive consumption, the involvement of brain regions has gained scientific relevance. In addition, a potential for fructose in biochemical changes and oxidative damage is observed. In this sense, the evaluation of biochemical alterations in the hypothalamus region and epididymal fat, in view of fructose consumption, may bring a better understanding of the evolution of some diseases with food involvement. Objective: To evaluate the effects of consumption of different fructose concentrations on biochemical parameters of mice. Methods: The experiment was based on water intake (ad libitum) with different fructose concentrations. Male Swiss mice were randomized into four groups, with exclusive intake of: water (H20 control), water + 5% fructose, water + 10% fructose and water + 20% fructose. The ration was common to all groups. Dietary intake and body weight were evaluated during the experiment. After 11 weeks the animals were euthanized for removal and analysis of visceral adipose tissue and hypothalamus. Results: There were no differences in body weight gain, but increased epididymal and visceral fat in fructose groups 10 and 20%. There was a tendency for increased oxidative damage to lipids in epididymal fat in fructose groups 10 and 20%, with alteration of mitochondrial respiratory chain complexes. In the hypothalamus fructose groups 10 and 20% presented oxidative damage, but without alteration of the mitochondrial respiratory chain. The same groups also showed a decrease in solid consumption and higher water intake. This contributed to the gradual increase in total fructose consumed. Conclusion: It was concluded that the consumption of higher amounts of fructose altered visceral fat deposition, were sufficient for mitochondrial changes in epididymal fat and increased oxidative damage in the hypothalamus.