Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos

Detalhes bibliográficos
Autor(a) principal: Cargnin-Carvalho, Anderson
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Universitário da Ânima (RUNA)
Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/3168
Resumo: Introduction: Urbanization has been dictating inadequate eating habits, with excessive consumption of sweetened drinks and high availability of fructose. From the different metabolic pathways involved in its excessive consumption, the involvement of brain regions has gained scientific relevance. In addition, a potential for fructose in biochemical changes and oxidative damage is observed. In this sense, the evaluation of biochemical alterations in the hypothalamus region and epididymal fat, in view of fructose consumption, may bring a better understanding of the evolution of some diseases with food involvement. Objective: To evaluate the effects of consumption of different fructose concentrations on biochemical parameters of mice. Methods: The experiment was based on water intake (ad libitum) with different fructose concentrations. Male Swiss mice were randomized into four groups, with exclusive intake of: water (H20 control), water + 5% fructose, water + 10% fructose and water + 20% fructose. The ration was common to all groups. Dietary intake and body weight were evaluated during the experiment. After 11 weeks the animals were euthanized for removal and analysis of visceral adipose tissue and hypothalamus. Results: There were no differences in body weight gain, but increased epididymal and visceral fat in fructose groups 10 and 20%. There was a tendency for increased oxidative damage to lipids in epididymal fat in fructose groups 10 and 20%, with alteration of mitochondrial respiratory chain complexes. In the hypothalamus fructose groups 10 and 20% presented oxidative damage, but without alteration of the mitochondrial respiratory chain. The same groups also showed a decrease in solid consumption and higher water intake. This contributed to the gradual increase in total fructose consumed. Conclusion: It was concluded that the consumption of higher amounts of fructose altered visceral fat deposition, were sufficient for mitochondrial changes in epididymal fat and increased oxidative damage in the hypothalamus.
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spelling Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongosFrutoseHipotálamoGorduraEstresse oxidativoIntroduction: Urbanization has been dictating inadequate eating habits, with excessive consumption of sweetened drinks and high availability of fructose. From the different metabolic pathways involved in its excessive consumption, the involvement of brain regions has gained scientific relevance. In addition, a potential for fructose in biochemical changes and oxidative damage is observed. In this sense, the evaluation of biochemical alterations in the hypothalamus region and epididymal fat, in view of fructose consumption, may bring a better understanding of the evolution of some diseases with food involvement. Objective: To evaluate the effects of consumption of different fructose concentrations on biochemical parameters of mice. Methods: The experiment was based on water intake (ad libitum) with different fructose concentrations. Male Swiss mice were randomized into four groups, with exclusive intake of: water (H20 control), water + 5% fructose, water + 10% fructose and water + 20% fructose. The ration was common to all groups. Dietary intake and body weight were evaluated during the experiment. After 11 weeks the animals were euthanized for removal and analysis of visceral adipose tissue and hypothalamus. Results: There were no differences in body weight gain, but increased epididymal and visceral fat in fructose groups 10 and 20%. There was a tendency for increased oxidative damage to lipids in epididymal fat in fructose groups 10 and 20%, with alteration of mitochondrial respiratory chain complexes. In the hypothalamus fructose groups 10 and 20% presented oxidative damage, but without alteration of the mitochondrial respiratory chain. The same groups also showed a decrease in solid consumption and higher water intake. This contributed to the gradual increase in total fructose consumed. Conclusion: It was concluded that the consumption of higher amounts of fructose altered visceral fat deposition, were sufficient for mitochondrial changes in epididymal fat and increased oxidative damage in the hypothalamus.Introdução: A urbanização vem ditando inadequações alimentares, com o consumo excessivo de bebidas adoçadas e grande disponibilidade de frutose. Das diferentes vias metabólicas envolvidas no seu consumo excessivo, o envolvimento de regiões cerebrais vem ganhando pertinência científica. Além disso, se observa um potencial da frutose em alterações bioquímicas e danos oxidativos. Nesse sentido, a avaliação de alterações bioquímicas na região do hipotálamo e na gordura epididimal, frente ao consumo de frutose, pode trazer um melhor compreendimento da evolução de algumas doenças com envolvimento alimentar. Objetivo: Avaliar os efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos. Métodos: O experimento se baseou na ingestão hídrica (ad libitum) com diferentes concentrações de frutose. Camundongos Swiss machos foram randomizados em quatro grupos, com ingestão exclusiva de: água (controle H20), água + 5% de frutose, água + 10% de frutose e água + 20% de frutose. A ração foi comum a todos os grupos. Foram avaliados o consumo alimentar e o peso corporal, durante o experimento. Após 11 semanas os animais foram eutanasiados, para remoção e análise do tecido adiposo visceral e do hipotálamo. Resultados: Não foram observadas diferenças no ganho de peso corporal, mas aumento da gordura epididimal e visceral, nos grupos frutose 10 e 20%. Houve uma tendência ao aumento do dano oxidativo a lipídeos na gordura epididimal, nos grupos frutose 10 e 20%, com alteração dos complexos da cadeia respiratória mitocondrial. No hipotálamo os grupos frutose 10 e 20%, apresentaram dano oxidativo, mas sem alteração da cadeia respiratória mitocondrial. Os mesmos grupos também apresentaram uma diminuição do consumo sólido e maior ingestão hídrica. O que contribuiu para o aumento gradativo do total de frutose consumida. Conclusão: Conclui-se que, o consumo das maiores quantidades de frutose, alteraram a deposição da gordura visceral, foram suficientes para alterações mitocondriais na gordura epididimal e aumentaram o dano oxidativo no hipotálamo.Rezin, Gislaine TezzaCargnin-Carvalho, Anderson2019-10-31T18:57:40Z2020-11-26T21:07:56Z2019-10-31T18:57:40Z2020-11-26T21:07:56Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis71 f.application/pdfhttps://repositorio.animaeducacao.com.br/handle/ANIMA/3168Programa de Pós-Graduação em Ciência da SaúdeTubarão - SCAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessporreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2020-12-01T16:50:06Zoai:repositorio.animaeducacao.com.br:ANIMA/3168Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2020-12-01T16:50:06Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse
dc.title.none.fl_str_mv Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
title Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
spellingShingle Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
Cargnin-Carvalho, Anderson
Frutose
Hipotálamo
Gordura
Estresse oxidativo
title_short Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
title_full Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
title_fullStr Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
title_full_unstemmed Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
title_sort Efeitos do consumo de diferentes concentrações de frutose sobre parâmetros bioquímicos de camundongos
author Cargnin-Carvalho, Anderson
author_facet Cargnin-Carvalho, Anderson
author_role author
dc.contributor.none.fl_str_mv Rezin, Gislaine Tezza
dc.contributor.author.fl_str_mv Cargnin-Carvalho, Anderson
dc.subject.por.fl_str_mv Frutose
Hipotálamo
Gordura
Estresse oxidativo
topic Frutose
Hipotálamo
Gordura
Estresse oxidativo
description Introduction: Urbanization has been dictating inadequate eating habits, with excessive consumption of sweetened drinks and high availability of fructose. From the different metabolic pathways involved in its excessive consumption, the involvement of brain regions has gained scientific relevance. In addition, a potential for fructose in biochemical changes and oxidative damage is observed. In this sense, the evaluation of biochemical alterations in the hypothalamus region and epididymal fat, in view of fructose consumption, may bring a better understanding of the evolution of some diseases with food involvement. Objective: To evaluate the effects of consumption of different fructose concentrations on biochemical parameters of mice. Methods: The experiment was based on water intake (ad libitum) with different fructose concentrations. Male Swiss mice were randomized into four groups, with exclusive intake of: water (H20 control), water + 5% fructose, water + 10% fructose and water + 20% fructose. The ration was common to all groups. Dietary intake and body weight were evaluated during the experiment. After 11 weeks the animals were euthanized for removal and analysis of visceral adipose tissue and hypothalamus. Results: There were no differences in body weight gain, but increased epididymal and visceral fat in fructose groups 10 and 20%. There was a tendency for increased oxidative damage to lipids in epididymal fat in fructose groups 10 and 20%, with alteration of mitochondrial respiratory chain complexes. In the hypothalamus fructose groups 10 and 20% presented oxidative damage, but without alteration of the mitochondrial respiratory chain. The same groups also showed a decrease in solid consumption and higher water intake. This contributed to the gradual increase in total fructose consumed. Conclusion: It was concluded that the consumption of higher amounts of fructose altered visceral fat deposition, were sufficient for mitochondrial changes in epididymal fat and increased oxidative damage in the hypothalamus.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-10-31T18:57:40Z
2019-10-31T18:57:40Z
2020-11-26T21:07:56Z
2020-11-26T21:07:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.animaeducacao.com.br/handle/ANIMA/3168
url https://repositorio.animaeducacao.com.br/handle/ANIMA/3168
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Programa de Pós-Graduação em Ciência da Saúde
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 71 f.
application/pdf
dc.coverage.none.fl_str_mv Tubarão - SC
dc.source.none.fl_str_mv reponame:Repositório Universitário da Ânima (RUNA)
instname:Ânima Educação
instacron:Ânima
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institution Ânima
reponame_str Repositório Universitário da Ânima (RUNA)
collection Repositório Universitário da Ânima (RUNA)
repository.name.fl_str_mv Repositório Universitário da Ânima (RUNA) - Ânima Educação
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