Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Douglas Zelinger
Data de Publicação: 2019
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Universitário da Ânima (RUNA)
Texto Completo: https://repositorio.animaeducacao.com.br/handle/ANIMA/12512
Resumo: The Aedes albopictus (Skuse, 1894) mosquito is considered a vector of medical importance because it has vector capacity in the laboratory for 22 arboviruses, including dengue, chikungunya and yellow fever viruses. The present study aimed to evaluate the larvicidal activity of the imidazolium salts (IS) C16MImMeS and C18MImCl against A. Albopictus larvae. For this, larvae of Ae. albopictus of 3rd final instar and/or 4th initial instar were exposed to 11 different concentrations (ranging from 0.5ppm to 10ppm) of C16MImMeS and C18MImCl. The bioassay was performed in triplicate, containing 20 larvae in each replicate and using purified water as control. It was conducted at a temperature of 25°C with a relative humidity of 80% (± 10%) in a climate chamber. Larval mortality was evaluated at 24 and 48 hours of exposure of the larvae to the solutions, being considered dead larvae that did not move when touched. For the results analysis, the variance test (ANOVA) was performed, with significant values of p≤0.05. Lethal concentrations, those that caused 50% mortality (LC50) were calculated by the Probit program. The IS C16MImMeS and C18MImCl showed larvicidal activity against A. albopictus at 24 and 48h exposure. At the lowest concentration (0.5ppm) of C16MImMeS and C18MImCl, mortality was 6.6 and 23.3%, at the highest concentration (10ppm) mortality was 85 and 100%, respectively. The 50% lethal concentrations (LC50) of IS C16MImMeS and C18MImCl at 48h exposure were 2.35ppm and 3.61ppm, respectively. Imidazolium salts C16MImMeS and C18MImCl have larvicidal potential against A. albopictus, being the first study to demonstrate the use of these IS to control A. albopictus. Further studies are necessary to understand its behavior in pupae and mosquito adulthood, its mechanism of action, and possible resistance factors.
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spelling Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)Aedes albopictusSais imidazólicosLarvicidaThe Aedes albopictus (Skuse, 1894) mosquito is considered a vector of medical importance because it has vector capacity in the laboratory for 22 arboviruses, including dengue, chikungunya and yellow fever viruses. The present study aimed to evaluate the larvicidal activity of the imidazolium salts (IS) C16MImMeS and C18MImCl against A. Albopictus larvae. For this, larvae of Ae. albopictus of 3rd final instar and/or 4th initial instar were exposed to 11 different concentrations (ranging from 0.5ppm to 10ppm) of C16MImMeS and C18MImCl. The bioassay was performed in triplicate, containing 20 larvae in each replicate and using purified water as control. It was conducted at a temperature of 25°C with a relative humidity of 80% (± 10%) in a climate chamber. Larval mortality was evaluated at 24 and 48 hours of exposure of the larvae to the solutions, being considered dead larvae that did not move when touched. For the results analysis, the variance test (ANOVA) was performed, with significant values of p≤0.05. Lethal concentrations, those that caused 50% mortality (LC50) were calculated by the Probit program. The IS C16MImMeS and C18MImCl showed larvicidal activity against A. albopictus at 24 and 48h exposure. At the lowest concentration (0.5ppm) of C16MImMeS and C18MImCl, mortality was 6.6 and 23.3%, at the highest concentration (10ppm) mortality was 85 and 100%, respectively. The 50% lethal concentrations (LC50) of IS C16MImMeS and C18MImCl at 48h exposure were 2.35ppm and 3.61ppm, respectively. Imidazolium salts C16MImMeS and C18MImCl have larvicidal potential against A. albopictus, being the first study to demonstrate the use of these IS to control A. albopictus. Further studies are necessary to understand its behavior in pupae and mosquito adulthood, its mechanism of action, and possible resistance factors.O mosquito Aedes albopictus (Skuse, 1894) é considerado vetor de importância médica por possuir capacidade vetorial em laboratório para 22 arbovírus, incluindo os vírus da dengue, chikungunya e da febre amarela. O presente estudo teve como objetivo avaliar a atividade larvicida dos sais imidazólicos (SI) C16MImMeS e C18MImCl, sobre larvas de A. albopictus. Para isso, larvas de A. albopictus de 3º instar final e/ou 4º instar inicial foram expostas a 11 diferentes concentrações (variando de 0,5ppm a 10ppm) de C16MImMeS e C18MImCl. O bioensaio foi realizado em triplicata, contendo 20 larvas em cada réplica e tendo como controle água purificada, sendo acondicionado à temperatura de 25°C com umidade relativa de 80% (±10%), em câmara climatizada. A mortalidade larval foi avaliada em 24 e 48 horas de exposição das larvas as soluções, sendo consideradas mortas as larvas que não se mexiam quando tocadas. Para análise dos resultados, foi realizado o teste de análise de variância (ANOVA), sendo significantes valores de p≤0,05. As concentrações letais que ocasionaram 50% de mortalidade (CL50) foram calculadas pelo programa Probit. Os SI C16MImMeS e C18MImCl apresentaram atividade larvicida sobre A. albopictus em 24 e 48h de exposição. Na menor concentração analisada (0,5ppm) de C16MImMeS e C18MImCl, a mortalidade foi de 6,6 e 23,3%, na maior concentração (10ppm) a mortalidade foi de 85 e 100%, respectivamente. As concentrações letais 50% (CL50) dos SI C16MImMeS e C18MImCl em 48h de exposição foram 2,35ppm e 3,61ppm, respectivamente. Os SI C16MImMeS e C18MImCl apresentam potencial larvicida contra A. albopictus, sendo o primeiro estudo a demonstrar o uso destes SI no controle de A. albopictus. Novos estudos são necessários para compreender seu comportamento em pupas e na fase adulta do mosquito, seu mecanismo de ação, e ainda, possíveis fatores de resistência.Prophiro, Josiane SomarivaGonçalves, Douglas Zelinger2020-08-11T18:51:06Z2020-12-09T17:19:22Z2020-08-11T18:51:06Z2020-12-09T17:19:22Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis39 fapplication/pdfhttps://repositorio.animaeducacao.com.br/handle/ANIMA/12512Ciências Biológicas Licenciatura - TubarãoTubarãoAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessporreponame:Repositório Universitário da Ânima (RUNA)instname:Ânima Educaçãoinstacron:Ânima2020-12-11T06:10:22Zoai:repositorio.animaeducacao.com.br:ANIMA/12512Repositório InstitucionalPRIhttps://repositorio.animaeducacao.com.br/oai/requestcontato@animaeducacao.com.bropendoar:2020-12-11T06:10:22Repositório Universitário da Ânima (RUNA) - Ânima Educaçãofalse
dc.title.none.fl_str_mv Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
title Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
spellingShingle Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
Gonçalves, Douglas Zelinger
Aedes albopictus
Sais imidazólicos
Larvicida
title_short Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
title_full Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
title_fullStr Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
title_full_unstemmed Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
title_sort Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)
author Gonçalves, Douglas Zelinger
author_facet Gonçalves, Douglas Zelinger
author_role author
dc.contributor.none.fl_str_mv Prophiro, Josiane Somariva
dc.contributor.author.fl_str_mv Gonçalves, Douglas Zelinger
dc.subject.por.fl_str_mv Aedes albopictus
Sais imidazólicos
Larvicida
topic Aedes albopictus
Sais imidazólicos
Larvicida
description The Aedes albopictus (Skuse, 1894) mosquito is considered a vector of medical importance because it has vector capacity in the laboratory for 22 arboviruses, including dengue, chikungunya and yellow fever viruses. The present study aimed to evaluate the larvicidal activity of the imidazolium salts (IS) C16MImMeS and C18MImCl against A. Albopictus larvae. For this, larvae of Ae. albopictus of 3rd final instar and/or 4th initial instar were exposed to 11 different concentrations (ranging from 0.5ppm to 10ppm) of C16MImMeS and C18MImCl. The bioassay was performed in triplicate, containing 20 larvae in each replicate and using purified water as control. It was conducted at a temperature of 25°C with a relative humidity of 80% (± 10%) in a climate chamber. Larval mortality was evaluated at 24 and 48 hours of exposure of the larvae to the solutions, being considered dead larvae that did not move when touched. For the results analysis, the variance test (ANOVA) was performed, with significant values of p≤0.05. Lethal concentrations, those that caused 50% mortality (LC50) were calculated by the Probit program. The IS C16MImMeS and C18MImCl showed larvicidal activity against A. albopictus at 24 and 48h exposure. At the lowest concentration (0.5ppm) of C16MImMeS and C18MImCl, mortality was 6.6 and 23.3%, at the highest concentration (10ppm) mortality was 85 and 100%, respectively. The 50% lethal concentrations (LC50) of IS C16MImMeS and C18MImCl at 48h exposure were 2.35ppm and 3.61ppm, respectively. Imidazolium salts C16MImMeS and C18MImCl have larvicidal potential against A. albopictus, being the first study to demonstrate the use of these IS to control A. albopictus. Further studies are necessary to understand its behavior in pupae and mosquito adulthood, its mechanism of action, and possible resistance factors.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-08-11T18:51:06Z
2020-12-09T17:19:22Z
2020-08-11T18:51:06Z
2020-12-09T17:19:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
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dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Ciências Biológicas Licenciatura - Tubarão
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 39 f
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