Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique

Detalhes bibliográficos
Autor(a) principal: GUERRA,PATRÍCIA A.
Data de Publicação: 2021
Outros Autores: SOUZA,EVERTON H., MAX,DANIELA A. S., ROSSI,MÔNICA L., VILLALOBOS-OLIVERA,ARIEL, LEDO,CARLOS A. S., MARTINEZ-MONTERO,MARCOS E., SOUZA,FERNANDA V. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501
Resumo: Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
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spelling Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification techniqueAnanas comosus (L.) MerrilEx situ conservationGrowing timeStarting materialRegenerationAbstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501Anais da Academia Brasileira de Ciências v.93 n.1 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120190555info:eu-repo/semantics/openAccessGUERRA,PATRÍCIA A.SOUZA,EVERTON H.MAX,DANIELA A. S.ROSSI,MÔNICA L.VILLALOBOS-OLIVERA,ARIELLEDO,CARLOS A. S.MARTINEZ-MONTERO,MARCOS E.SOUZA,FERNANDA V. D.eng2021-03-03T00:00:00Zoai:scielo:S0001-37652021000101501Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-03-03T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
title Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
spellingShingle Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
GUERRA,PATRÍCIA A.
Ananas comosus (L.) Merril
Ex situ conservation
Growing time
Starting material
Regeneration
title_short Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
title_full Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
title_fullStr Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
title_full_unstemmed Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
title_sort Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique
author GUERRA,PATRÍCIA A.
author_facet GUERRA,PATRÍCIA A.
SOUZA,EVERTON H.
MAX,DANIELA A. S.
ROSSI,MÔNICA L.
VILLALOBOS-OLIVERA,ARIEL
LEDO,CARLOS A. S.
MARTINEZ-MONTERO,MARCOS E.
SOUZA,FERNANDA V. D.
author_role author
author2 SOUZA,EVERTON H.
MAX,DANIELA A. S.
ROSSI,MÔNICA L.
VILLALOBOS-OLIVERA,ARIEL
LEDO,CARLOS A. S.
MARTINEZ-MONTERO,MARCOS E.
SOUZA,FERNANDA V. D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv GUERRA,PATRÍCIA A.
SOUZA,EVERTON H.
MAX,DANIELA A. S.
ROSSI,MÔNICA L.
VILLALOBOS-OLIVERA,ARIEL
LEDO,CARLOS A. S.
MARTINEZ-MONTERO,MARCOS E.
SOUZA,FERNANDA V. D.
dc.subject.por.fl_str_mv Ananas comosus (L.) Merril
Ex situ conservation
Growing time
Starting material
Regeneration
topic Ananas comosus (L.) Merril
Ex situ conservation
Growing time
Starting material
Regeneration
description Abstract Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 ( var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202120190555
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.93 n.1 2021
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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