Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599 |
Resumo: | ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by introducing a genome completely synthesized within a cell envelope of a mycoplasma obtained by transformation techniques. Therefore, this article offers an updated insight to the state of the art of these peculiar organisms' molecular biology. |
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Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cellmycoplasmamolecular biologymicrobial geneticssynthetic biologygene transfer techniquesABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by introducing a genome completely synthesized within a cell envelope of a mycoplasma obtained by transformation techniques. Therefore, this article offers an updated insight to the state of the art of these peculiar organisms' molecular biology.Academia Brasileira de Ciências2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599Anais da Academia Brasileira de Ciências v.88 suppl.1 2016reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201620150164info:eu-repo/semantics/openAccessCORDOVA,CAIO M.M.HOELTGEBAUM,DANIELA L.MACHADO,LAÍS D.P.N.SANTOS,LARISSA DOSeng2016-06-17T00:00:00Zoai:scielo:S0001-37652016000200599Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-06-17T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
title |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
spellingShingle |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell CORDOVA,CAIO M.M. mycoplasma molecular biology microbial genetics synthetic biology gene transfer techniques |
title_short |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
title_full |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
title_fullStr |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
title_full_unstemmed |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
title_sort |
Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell |
author |
CORDOVA,CAIO M.M. |
author_facet |
CORDOVA,CAIO M.M. HOELTGEBAUM,DANIELA L. MACHADO,LAÍS D.P.N. SANTOS,LARISSA DOS |
author_role |
author |
author2 |
HOELTGEBAUM,DANIELA L. MACHADO,LAÍS D.P.N. SANTOS,LARISSA DOS |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
CORDOVA,CAIO M.M. HOELTGEBAUM,DANIELA L. MACHADO,LAÍS D.P.N. SANTOS,LARISSA DOS |
dc.subject.por.fl_str_mv |
mycoplasma molecular biology microbial genetics synthetic biology gene transfer techniques |
topic |
mycoplasma molecular biology microbial genetics synthetic biology gene transfer techniques |
description |
ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by introducing a genome completely synthesized within a cell envelope of a mycoplasma obtained by transformation techniques. Therefore, this article offers an updated insight to the state of the art of these peculiar organisms' molecular biology. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201620150164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.88 suppl.1 2016 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302862790230016 |