Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0022029920000205 http://hdl.handle.net/11449/200511 |
Resumo: | The work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence. |
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Detection of clinical bovine mastitis caused by Mycoplasma bovis in BrazilMastitismicrobiologymolecular biologyMycoplasma bovisMycoplasmosisThe work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence.Department of Veterinary Hygiene and Public Health São Paulo State University (Unesp) School of Veterinary Medicine and Animal ScienceLaboratti Veterinary Diagnostic Laboratory Pedrinhas PaulistaDepartment of Veterinary Hygiene and Public Health São Paulo State University (Unesp) School of Veterinary Medicine and Animal ScienceUniversidade Estadual Paulista (Unesp)Pedrinhas PaulistaJunqueira, Nathália Brancato [UNESP]Salina, Anelise [UNESP]Oliveira, Gabriela Capriogli [UNESP]Mettifogo, ElenaJoaquim, Sâmea Fernandes [UNESP]Guimarães, Felipe Freitas [UNESP]Dalanezi, Felipe Morales [UNESP]Langoni, Helio [UNESP]2020-12-12T02:08:32Z2020-12-12T02:08:32Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S0022029920000205Journal of Dairy Research.1469-76290022-0299http://hdl.handle.net/11449/20051110.1017/S00220299200002052-s2.0-85085322305Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Researchinfo:eu-repo/semantics/openAccess2021-10-23T14:33:47Zoai:repositorio.unesp.br:11449/200511Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:33:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
title |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
spellingShingle |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil Junqueira, Nathália Brancato [UNESP] Mastitis microbiology molecular biology Mycoplasma bovis Mycoplasmosis |
title_short |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
title_full |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
title_fullStr |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
title_full_unstemmed |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
title_sort |
Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil |
author |
Junqueira, Nathália Brancato [UNESP] |
author_facet |
Junqueira, Nathália Brancato [UNESP] Salina, Anelise [UNESP] Oliveira, Gabriela Capriogli [UNESP] Mettifogo, Elena Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] Dalanezi, Felipe Morales [UNESP] Langoni, Helio [UNESP] |
author_role |
author |
author2 |
Salina, Anelise [UNESP] Oliveira, Gabriela Capriogli [UNESP] Mettifogo, Elena Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] Dalanezi, Felipe Morales [UNESP] Langoni, Helio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Pedrinhas Paulista |
dc.contributor.author.fl_str_mv |
Junqueira, Nathália Brancato [UNESP] Salina, Anelise [UNESP] Oliveira, Gabriela Capriogli [UNESP] Mettifogo, Elena Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] Dalanezi, Felipe Morales [UNESP] Langoni, Helio [UNESP] |
dc.subject.por.fl_str_mv |
Mastitis microbiology molecular biology Mycoplasma bovis Mycoplasmosis |
topic |
Mastitis microbiology molecular biology Mycoplasma bovis Mycoplasmosis |
description |
The work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:08:32Z 2020-12-12T02:08:32Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0022029920000205 Journal of Dairy Research. 1469-7629 0022-0299 http://hdl.handle.net/11449/200511 10.1017/S0022029920000205 2-s2.0-85085322305 |
url |
http://dx.doi.org/10.1017/S0022029920000205 http://hdl.handle.net/11449/200511 |
identifier_str_mv |
Journal of Dairy Research. 1469-7629 0022-0299 10.1017/S0022029920000205 2-s2.0-85085322305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dairy Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964657935450112 |