Are salty liquid food flavorings in vitro antitumor substances?

Detalhes bibliográficos
Autor(a) principal: CARVALHO,FRANCISCO R.S.
Data de Publicação: 2016
Outros Autores: MOURA,ANTONIO G., RODRIGUES,GARDENIA F., NUNES,NARCIA M.F., LIMA,DAISY J.B., PESSOA,CLAUDIA, COSTA,MARCILIA P., FERREIRA,PAULO M.P., PERON,ANA PAULA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419
Resumo: ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.
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spelling Are salty liquid food flavorings in vitro antitumor substances?food additivesAllium cepaantiproliferative actionneoplasic cellstoxicityABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.Academia Brasileira de Ciências2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419Anais da Academia Brasileira de Ciências v.88 n.3 2016reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201620150553info:eu-repo/semantics/openAccessCARVALHO,FRANCISCO R.S.MOURA,ANTONIO G.RODRIGUES,GARDENIA F.NUNES,NARCIA M.F.LIMA,DAISY J.B.PESSOA,CLAUDIACOSTA,MARCILIA P.FERREIRA,PAULO M.P.PERON,ANA PAULAeng2016-09-12T00:00:00Zoai:scielo:S0001-37652016000401419Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-09-12T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Are salty liquid food flavorings in vitro antitumor substances?
title Are salty liquid food flavorings in vitro antitumor substances?
spellingShingle Are salty liquid food flavorings in vitro antitumor substances?
CARVALHO,FRANCISCO R.S.
food additives
Allium cepa
antiproliferative action
neoplasic cells
toxicity
title_short Are salty liquid food flavorings in vitro antitumor substances?
title_full Are salty liquid food flavorings in vitro antitumor substances?
title_fullStr Are salty liquid food flavorings in vitro antitumor substances?
title_full_unstemmed Are salty liquid food flavorings in vitro antitumor substances?
title_sort Are salty liquid food flavorings in vitro antitumor substances?
author CARVALHO,FRANCISCO R.S.
author_facet CARVALHO,FRANCISCO R.S.
MOURA,ANTONIO G.
RODRIGUES,GARDENIA F.
NUNES,NARCIA M.F.
LIMA,DAISY J.B.
PESSOA,CLAUDIA
COSTA,MARCILIA P.
FERREIRA,PAULO M.P.
PERON,ANA PAULA
author_role author
author2 MOURA,ANTONIO G.
RODRIGUES,GARDENIA F.
NUNES,NARCIA M.F.
LIMA,DAISY J.B.
PESSOA,CLAUDIA
COSTA,MARCILIA P.
FERREIRA,PAULO M.P.
PERON,ANA PAULA
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv CARVALHO,FRANCISCO R.S.
MOURA,ANTONIO G.
RODRIGUES,GARDENIA F.
NUNES,NARCIA M.F.
LIMA,DAISY J.B.
PESSOA,CLAUDIA
COSTA,MARCILIA P.
FERREIRA,PAULO M.P.
PERON,ANA PAULA
dc.subject.por.fl_str_mv food additives
Allium cepa
antiproliferative action
neoplasic cells
toxicity
topic food additives
Allium cepa
antiproliferative action
neoplasic cells
toxicity
description ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201620150553
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.88 n.3 2016
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
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instname_str Academia Brasileira de Ciências (ABC)
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institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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