Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses

Detalhes bibliográficos
Autor(a) principal: SILVA,RAIMUNDO DA
Data de Publicação: 2017
Outros Autores: SILVA,DANILLO, VENEZA,IVANA, SAMPAIO,IRACILDA, SCHNEIDER,HORACIO, GOMES,GRAZIELLE
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502095
Resumo: ABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa.
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spelling Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population AnalysesEPIC-PCRintronLutjanusSouthern Red SnapperABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa.Academia Brasileira de Ciências2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502095Anais da Academia Brasileira de Ciências v.89 n.3 suppl.0 2017reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720150476info:eu-repo/semantics/openAccessSILVA,RAIMUNDO DASILVA,DANILLOVENEZA,IVANASAMPAIO,IRACILDASCHNEIDER,HORACIOGOMES,GRAZIELLEeng2017-11-13T00:00:00Zoai:scielo:S0001-37652017000502095Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2017-11-13T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
title Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
spellingShingle Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
SILVA,RAIMUNDO DA
EPIC-PCR
intron
Lutjanus
Southern Red Snapper
title_short Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
title_full Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
title_fullStr Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
title_full_unstemmed Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
title_sort Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
author SILVA,RAIMUNDO DA
author_facet SILVA,RAIMUNDO DA
SILVA,DANILLO
VENEZA,IVANA
SAMPAIO,IRACILDA
SCHNEIDER,HORACIO
GOMES,GRAZIELLE
author_role author
author2 SILVA,DANILLO
VENEZA,IVANA
SAMPAIO,IRACILDA
SCHNEIDER,HORACIO
GOMES,GRAZIELLE
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv SILVA,RAIMUNDO DA
SILVA,DANILLO
VENEZA,IVANA
SAMPAIO,IRACILDA
SCHNEIDER,HORACIO
GOMES,GRAZIELLE
dc.subject.por.fl_str_mv EPIC-PCR
intron
Lutjanus
Southern Red Snapper
topic EPIC-PCR
intron
Lutjanus
Southern Red Snapper
description ABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502095
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502095
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201720150476
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.89 n.3 suppl.0 2017
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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