Plasma miR-1273g-3p acts as a potential biomarker for early Breast Ductal Cancer diagnosis

• Autor(a) principal: Guo,Huan
• Data de Publicação: 2020
• Outros Autores: Zeng,Xiangting, Li,Haining, Guo,Yanxiang, Wang,Tao, Guo,Hongyun, Zhu,Gongjian, Wang,Lan, Zhou,Haihong, Liu,Kedan, Chen,Xiaoyi, Wang,Haitao, Zhao,Xinke, Su,Haixiang, Li,Yingdong
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Anais da Academia Brasileira de Ciências (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100801
• topic: breast ductal carcinoma; plasma; miR-1273g-3p; circulating miRNA; biomarker

Abstract Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer’s disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also revealed the significantly diagnostic ability of miR-1273g-3p in patients (p=0.0414). In addition, the plasma level of miR-1273g-3p was closely related to IIIB-IIIC TNM stage. We also confirmed the higher expression level of miR-1273g-3p in breast cancer cell lines MCF-7 (4.872±0.537) than normal breast cells (Hs 578Bst). Taken together, miR-1273g-3p could represent as a potential biomarker for early breast ductal cancer diagnosis.