Searching an auxinic herbicide to use as positive control in toxicity assays
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101504 |
Resumo: | Abstract Due to rising concerns for environmental and human health, many toxic compounds, such as auxin-based herbicides, have been tested in relation their toxicity effect. Especially cyto- and phytotoxic assays have been performed on a number monocot and eudicot plant species. In these approaches the toxicity level of the auxin is compared to a positive control – usually a commercial compound with known effects and chemical similarity to the target compound. However, many target compounds still lack an indication of an adequate positive control. Here, we evaluate the phytotoxic and cytotoxic effect of the auxins 2,4-dichlorophenoxyacetic acid, dicamba, and picloram in order test their potential use as positive controls. All tested auxinic herbicides showed clastogenic and aneugenic effect mechanisms. The results indicate 2,4-dichlorophenoxyacetic acid as the most phyto- and cytotoxic in the discontinuous method in Lactuca sativa L. and Allium cepa L., and also in the continuous method in A. cepa. Thus, we suggest 2,4-dichlorophenoxyacetic acid as a positive control for future mutagenesis studies involving new auxins. For studies with L. sativa in continuous method, we recommend the auxin picloram as positive control as this one was the only one which allowed the development of roots. |
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Searching an auxinic herbicide to use as positive control in toxicity assays24-DcytotoxicitydicambamutagenesisphytotoxicitypicloramAbstract Due to rising concerns for environmental and human health, many toxic compounds, such as auxin-based herbicides, have been tested in relation their toxicity effect. Especially cyto- and phytotoxic assays have been performed on a number monocot and eudicot plant species. In these approaches the toxicity level of the auxin is compared to a positive control – usually a commercial compound with known effects and chemical similarity to the target compound. However, many target compounds still lack an indication of an adequate positive control. Here, we evaluate the phytotoxic and cytotoxic effect of the auxins 2,4-dichlorophenoxyacetic acid, dicamba, and picloram in order test their potential use as positive controls. All tested auxinic herbicides showed clastogenic and aneugenic effect mechanisms. The results indicate 2,4-dichlorophenoxyacetic acid as the most phyto- and cytotoxic in the discontinuous method in Lactuca sativa L. and Allium cepa L., and also in the continuous method in A. cepa. Thus, we suggest 2,4-dichlorophenoxyacetic acid as a positive control for future mutagenesis studies involving new auxins. For studies with L. sativa in continuous method, we recommend the auxin picloram as positive control as this one was the only one which allowed the development of roots.Academia Brasileira de Ciências2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101504Anais da Academia Brasileira de Ciências v.93 n.1 2021reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202120181262info:eu-repo/semantics/openAccessALVES,THAMMYRES A.ROBERTO,CARLOS EDUARDO O.PINHEIRO,PATRÍCIA F.ALVES,THAYLLON A.HENRIQUE,MAIKON K.C.FERREIRA,ADÉSIOCLARINDO,WELLINGTON R.PRAÇA-FONTES,MILENE M.eng2021-03-24T00:00:00Zoai:scielo:S0001-37652021000101504Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2021-03-24T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Searching an auxinic herbicide to use as positive control in toxicity assays |
title |
Searching an auxinic herbicide to use as positive control in toxicity assays |
spellingShingle |
Searching an auxinic herbicide to use as positive control in toxicity assays ALVES,THAMMYRES A. 2 4-D cytotoxicity dicamba mutagenesis phytotoxicity picloram |
title_short |
Searching an auxinic herbicide to use as positive control in toxicity assays |
title_full |
Searching an auxinic herbicide to use as positive control in toxicity assays |
title_fullStr |
Searching an auxinic herbicide to use as positive control in toxicity assays |
title_full_unstemmed |
Searching an auxinic herbicide to use as positive control in toxicity assays |
title_sort |
Searching an auxinic herbicide to use as positive control in toxicity assays |
author |
ALVES,THAMMYRES A. |
author_facet |
ALVES,THAMMYRES A. ROBERTO,CARLOS EDUARDO O. PINHEIRO,PATRÍCIA F. ALVES,THAYLLON A. HENRIQUE,MAIKON K.C. FERREIRA,ADÉSIO CLARINDO,WELLINGTON R. PRAÇA-FONTES,MILENE M. |
author_role |
author |
author2 |
ROBERTO,CARLOS EDUARDO O. PINHEIRO,PATRÍCIA F. ALVES,THAYLLON A. HENRIQUE,MAIKON K.C. FERREIRA,ADÉSIO CLARINDO,WELLINGTON R. PRAÇA-FONTES,MILENE M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
ALVES,THAMMYRES A. ROBERTO,CARLOS EDUARDO O. PINHEIRO,PATRÍCIA F. ALVES,THAYLLON A. HENRIQUE,MAIKON K.C. FERREIRA,ADÉSIO CLARINDO,WELLINGTON R. PRAÇA-FONTES,MILENE M. |
dc.subject.por.fl_str_mv |
2 4-D cytotoxicity dicamba mutagenesis phytotoxicity picloram |
topic |
2 4-D cytotoxicity dicamba mutagenesis phytotoxicity picloram |
description |
Abstract Due to rising concerns for environmental and human health, many toxic compounds, such as auxin-based herbicides, have been tested in relation their toxicity effect. Especially cyto- and phytotoxic assays have been performed on a number monocot and eudicot plant species. In these approaches the toxicity level of the auxin is compared to a positive control – usually a commercial compound with known effects and chemical similarity to the target compound. However, many target compounds still lack an indication of an adequate positive control. Here, we evaluate the phytotoxic and cytotoxic effect of the auxins 2,4-dichlorophenoxyacetic acid, dicamba, and picloram in order test their potential use as positive controls. All tested auxinic herbicides showed clastogenic and aneugenic effect mechanisms. The results indicate 2,4-dichlorophenoxyacetic acid as the most phyto- and cytotoxic in the discontinuous method in Lactuca sativa L. and Allium cepa L., and also in the continuous method in A. cepa. Thus, we suggest 2,4-dichlorophenoxyacetic acid as a positive control for future mutagenesis studies involving new auxins. For studies with L. sativa in continuous method, we recommend the auxin picloram as positive control as this one was the only one which allowed the development of roots. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101504 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000101504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765202120181262 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.93 n.1 2021 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302869746483200 |