In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review

Detalhes bibliográficos
Autor(a) principal: CECHIM,GIOVANA
Data de Publicação: 2019
Outros Autores: CHIES,JOSÉ A.B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700614
Resumo: Abstract: Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune system. As a result of such studies, monocyte-derived dendritic cells (MDDC) are presently used in clinical protocols for the treatment of a variety of diseases, including cancer and human immunodeficiency virus infection. We summarize recent advances in the understanding of methodologies and inputs used in protocols to differentiate DCs from blood monocytes in vitro.
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spelling In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive reviewCytokinesdendritic celldifferentiationmonocytesAbstract: Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune system. As a result of such studies, monocyte-derived dendritic cells (MDDC) are presently used in clinical protocols for the treatment of a variety of diseases, including cancer and human immunodeficiency virus infection. We summarize recent advances in the understanding of methodologies and inputs used in protocols to differentiate DCs from blood monocytes in vitro.Academia Brasileira de Ciências2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700614Anais da Academia Brasileira de Ciências v.91 n.4 2019reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201920190310info:eu-repo/semantics/openAccessCECHIM,GIOVANACHIES,JOSÉ A.B.eng2019-11-26T00:00:00Zoai:scielo:S0001-37652019000700614Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-11-26T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
title In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
spellingShingle In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
CECHIM,GIOVANA
Cytokines
dendritic cell
differentiation
monocytes
title_short In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
title_full In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
title_fullStr In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
title_full_unstemmed In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
title_sort In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
author CECHIM,GIOVANA
author_facet CECHIM,GIOVANA
CHIES,JOSÉ A.B.
author_role author
author2 CHIES,JOSÉ A.B.
author2_role author
dc.contributor.author.fl_str_mv CECHIM,GIOVANA
CHIES,JOSÉ A.B.
dc.subject.por.fl_str_mv Cytokines
dendritic cell
differentiation
monocytes
topic Cytokines
dendritic cell
differentiation
monocytes
description Abstract: Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune system. As a result of such studies, monocyte-derived dendritic cells (MDDC) are presently used in clinical protocols for the treatment of a variety of diseases, including cancer and human immunodeficiency virus infection. We summarize recent advances in the understanding of methodologies and inputs used in protocols to differentiate DCs from blood monocytes in vitro.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700614
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201920190310
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dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.91 n.4 2019
reponame:Anais da Academia Brasileira de Ciências (Online)
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