Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502001 |
Resumo: | ABSTRACT We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds. |
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Anais da Academia Brasileira de Ciências (Online) |
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Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seedscell cycledesiccation sensitivityDNA contentdryingheat-stable proteinsABSTRACT We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.Academia Brasileira de Ciências2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502001Anais da Academia Brasileira de Ciências v.87 n.4 2015reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201520140195info:eu-repo/semantics/openAccessSOARES,GIULIANA C.M.DIAS,DENISE C.F.S.FARIA,JOSÉ M.R.BORGES,EDUARDO E.L.eng2015-12-11T00:00:00Zoai:scielo:S0001-37652015000502001Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-12-11T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
title |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
spellingShingle |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds SOARES,GIULIANA C.M. cell cycle desiccation sensitivity DNA content drying heat-stable proteins |
title_short |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
title_full |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
title_fullStr |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
title_full_unstemmed |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
title_sort |
Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds |
author |
SOARES,GIULIANA C.M. |
author_facet |
SOARES,GIULIANA C.M. DIAS,DENISE C.F.S. FARIA,JOSÉ M.R. BORGES,EDUARDO E.L. |
author_role |
author |
author2 |
DIAS,DENISE C.F.S. FARIA,JOSÉ M.R. BORGES,EDUARDO E.L. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
SOARES,GIULIANA C.M. DIAS,DENISE C.F.S. FARIA,JOSÉ M.R. BORGES,EDUARDO E.L. |
dc.subject.por.fl_str_mv |
cell cycle desiccation sensitivity DNA content drying heat-stable proteins |
topic |
cell cycle desiccation sensitivity DNA content drying heat-stable proteins |
description |
ABSTRACT We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201520140195 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.87 n.4 2015 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302861237288960 |