Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds

Detalhes bibliográficos
Autor(a) principal: Soares, Giuliana C.M.
Data de Publicação: 2015
Outros Autores: Dias, Denise C.F.S., Faria, José M.R., Borges, Eduardo E.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/0001-3765201520140195
http://www.locus.ufv.br/handle/123456789/12528
Resumo: We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.
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spelling Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seedsCell cycleDesiccation sensitivityDNA contentDryingHeat-stable proteinsWe investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.Nós investigamos a perda da tolerância à dessecação (TD) em sementes deAdenanthera pavonina durante a germinação. Sementes submetidas à embebição por 0, 24, 36, 48, 60 e 81 h foram secas até à umidade inicial (13%), reidratadas e avaliadas quanto à sobrevivência (retomada do crescimento e formação de plântulas normais) e à integridade do sistema de membranas (extravasamento de eletrólitos). Eixos embrionários de sementes submetidas apenas à embebição, pelos mesmos tempos citados anteriormente, foram utilizados para avaliar os padrões eletroforéticos de proteínas resistentes ao calor e o conteúdo relativo de DNA nuclear. Em sementes de A. pavonina, a TD foi mantida por até 36 h de embebição (resultando em germinação e 82% de plântulas normais), após o que foi gradativamente perdida, até que sementes com 1 mm de raiz primária não sobreviveram após a secagem. A perda da TD não pôde ser correlacionada com danos causados por secagem ao sistema de membranas, nem com a retomada do ciclo celular durante a germinação. No entanto, a redução do conteúdo de proteínas resistentes ao calor, ao longo da germinação, pode estar relacionada com a perda da TD em sementes de A. pavonina.Anais da Academia Brasileira de Ciências2017-10-30T15:43:14Z2017-10-30T15:43:14Z2015-01-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1678-2690http://dx.doi.org/10.1590/0001-3765201520140195http://www.locus.ufv.br/handle/123456789/12528engv.87 n.4, p. 2001-2011, Oct./Dec. 2015Soares, Giuliana C.M.Dias, Denise C.F.S.Faria, José M.R.Borges, Eduardo E.L.info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:02:29Zoai:locus.ufv.br:123456789/12528Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:02:29LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
title Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
spellingShingle Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
Soares, Giuliana C.M.
Cell cycle
Desiccation sensitivity
DNA content
Drying
Heat-stable proteins
title_short Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
title_full Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
title_fullStr Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
title_full_unstemmed Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
title_sort Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds
author Soares, Giuliana C.M.
author_facet Soares, Giuliana C.M.
Dias, Denise C.F.S.
Faria, José M.R.
Borges, Eduardo E.L.
author_role author
author2 Dias, Denise C.F.S.
Faria, José M.R.
Borges, Eduardo E.L.
author2_role author
author
author
dc.contributor.author.fl_str_mv Soares, Giuliana C.M.
Dias, Denise C.F.S.
Faria, José M.R.
Borges, Eduardo E.L.
dc.subject.por.fl_str_mv Cell cycle
Desiccation sensitivity
DNA content
Drying
Heat-stable proteins
topic Cell cycle
Desiccation sensitivity
DNA content
Drying
Heat-stable proteins
description We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-16
2017-10-30T15:43:14Z
2017-10-30T15:43:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1678-2690
http://dx.doi.org/10.1590/0001-3765201520140195
http://www.locus.ufv.br/handle/123456789/12528
identifier_str_mv 1678-2690
url http://dx.doi.org/10.1590/0001-3765201520140195
http://www.locus.ufv.br/handle/123456789/12528
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v.87 n.4, p. 2001-2011, Oct./Dec. 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Anais da Academia Brasileira de Ciências
publisher.none.fl_str_mv Anais da Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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