Natural sesquiterpen lactones as acetylcholinesterase inhibitors

Detalhes bibliográficos
Autor(a) principal: HAJIMEHDIPOOR,HOMA
Data de Publicação: 2014
Outros Autores: MOSADDEGH,MAHMOUD, NAGHIBI,FARZANEH, HAERI,ALI, HAMZELOO-MOGHADAM,MARYAM
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200801
Resumo: Background and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5′-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 µg/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.
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spelling Natural sesquiterpen lactones as acetylcholinesterase inhibitorsAcetylcholinesterase inhibitorAlzheimerbritanningaillardinInulapulchellin CBackground and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5′-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 µg/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.Academia Brasileira de Ciências2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200801Anais da Academia Brasileira de Ciências v.86 n.2 2014reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201420130005info:eu-repo/semantics/openAccessHAJIMEHDIPOOR,HOMAMOSADDEGH,MAHMOUDNAGHIBI,FARZANEHHAERI,ALIHAMZELOO-MOGHADAM,MARYAMeng2015-10-26T00:00:00Zoai:scielo:S0001-37652014000200801Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2015-10-26T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Natural sesquiterpen lactones as acetylcholinesterase inhibitors
title Natural sesquiterpen lactones as acetylcholinesterase inhibitors
spellingShingle Natural sesquiterpen lactones as acetylcholinesterase inhibitors
HAJIMEHDIPOOR,HOMA
Acetylcholinesterase inhibitor
Alzheimer
britannin
gaillardin
Inula
pulchellin C
title_short Natural sesquiterpen lactones as acetylcholinesterase inhibitors
title_full Natural sesquiterpen lactones as acetylcholinesterase inhibitors
title_fullStr Natural sesquiterpen lactones as acetylcholinesterase inhibitors
title_full_unstemmed Natural sesquiterpen lactones as acetylcholinesterase inhibitors
title_sort Natural sesquiterpen lactones as acetylcholinesterase inhibitors
author HAJIMEHDIPOOR,HOMA
author_facet HAJIMEHDIPOOR,HOMA
MOSADDEGH,MAHMOUD
NAGHIBI,FARZANEH
HAERI,ALI
HAMZELOO-MOGHADAM,MARYAM
author_role author
author2 MOSADDEGH,MAHMOUD
NAGHIBI,FARZANEH
HAERI,ALI
HAMZELOO-MOGHADAM,MARYAM
author2_role author
author
author
author
dc.contributor.author.fl_str_mv HAJIMEHDIPOOR,HOMA
MOSADDEGH,MAHMOUD
NAGHIBI,FARZANEH
HAERI,ALI
HAMZELOO-MOGHADAM,MARYAM
dc.subject.por.fl_str_mv Acetylcholinesterase inhibitor
Alzheimer
britannin
gaillardin
Inula
pulchellin C
topic Acetylcholinesterase inhibitor
Alzheimer
britannin
gaillardin
Inula
pulchellin C
description Background and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5′-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 µg/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200801
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201420130005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.86 n.2 2014
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
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reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
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