FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117 |
Resumo: | ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. |
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Anais da Academia Brasileira de Ciências (Online) |
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FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)wild and cultivated Capsicum5S rRNA geneCapsicum-derived probeFISH-mappingABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.Academia Brasileira de Ciências2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117Anais da Academia Brasileira de Ciências v.88 n.1 2016reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-37652301620140616info:eu-repo/semantics/openAccessAGUILERA,PATRICIA M.DEBAT,HUMBERTO J.SCALDAFERRO,MARISEL A.MARTÍ,DARDO A.GRABIELE,MAUROeng2016-03-07T00:00:00Zoai:scielo:S0001-37652016000100117Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2016-03-07T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
spellingShingle |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) AGUILERA,PATRICIA M. wild and cultivated Capsicum 5S rRNA gene Capsicum-derived probe FISH-mapping |
title_short |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_full |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_fullStr |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_full_unstemmed |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_sort |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
author |
AGUILERA,PATRICIA M. |
author_facet |
AGUILERA,PATRICIA M. DEBAT,HUMBERTO J. SCALDAFERRO,MARISEL A. MARTÍ,DARDO A. GRABIELE,MAURO |
author_role |
author |
author2 |
DEBAT,HUMBERTO J. SCALDAFERRO,MARISEL A. MARTÍ,DARDO A. GRABIELE,MAURO |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
AGUILERA,PATRICIA M. DEBAT,HUMBERTO J. SCALDAFERRO,MARISEL A. MARTÍ,DARDO A. GRABIELE,MAURO |
dc.subject.por.fl_str_mv |
wild and cultivated Capsicum 5S rRNA gene Capsicum-derived probe FISH-mapping |
topic |
wild and cultivated Capsicum 5S rRNA gene Capsicum-derived probe FISH-mapping |
description |
ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-37652301620140616 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.88 n.1 2016 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
_version_ |
1754302862774501376 |