Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

Detalhes bibliográficos
Autor(a) principal: Avram,Ş.F.
Data de Publicação: 2013
Outros Autores: Cimpean,A.M., Raica,M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100052
Resumo: The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.
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spelling Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryosP1.HTRMastocytomaChick embryo chorioallantoic membraneAngiogenesisThe P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.Associação Brasileira de Divulgação Científica2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100052Brazilian Journal of Medical and Biological Research v.46 n.1 2013reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431X20122434info:eu-repo/semantics/openAccessAvram,Ş.F.Cimpean,A.M.Raica,M.eng2015-07-21T00:00:00Zoai:scielo:S0100-879X2013000100052Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2015-07-21T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
title Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
spellingShingle Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
Avram,Ş.F.
P1.HTR
Mastocytoma
Chick embryo chorioallantoic membrane
Angiogenesis
title_short Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
title_full Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
title_fullStr Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
title_full_unstemmed Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
title_sort Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
author Avram,Ş.F.
author_facet Avram,Ş.F.
Cimpean,A.M.
Raica,M.
author_role author
author2 Cimpean,A.M.
Raica,M.
author2_role author
author
dc.contributor.author.fl_str_mv Avram,Ş.F.
Cimpean,A.M.
Raica,M.
dc.subject.por.fl_str_mv P1.HTR
Mastocytoma
Chick embryo chorioallantoic membrane
Angiogenesis
topic P1.HTR
Mastocytoma
Chick embryo chorioallantoic membrane
Angiogenesis
description The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100052
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100052
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431X20122434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.46 n.1 2013
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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