The role of enzymes in the angiogenic activity of Hancornia speciosa latex

Detalhes bibliográficos
Autor(a) principal: D'Abadia, Patricia
Data de Publicação: 2022
Outros Autores: Fernandes Costa, Amanda, Firmino, Myllena Tolentino Firmino, Caramori, Samantha, Ruy de Souza Lino-Junior, Zanoelo, Fabiana Fonseca Zanoelo, Gonçalves, Pablo José Gonçalves, Almeida, Luciane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/61092
Resumo: The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.
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spelling The role of enzymes in the angiogenic activity of Hancornia speciosa latexO papel das enzimas na atividade angiogênica do látex de Hancornia speciosaChick chorioallantoic membraneEnzymesProteinase KBiological SciencesThe Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.O látex de Hancornia speciosa possui atividade angiogênica, antiinflamatória e antioxidante. Essas propriedades são desejáveis para medicamentos usados para auxiliar na cicatrização de feridas. A angiogênese, foco desta pesquisa, é uma etapa crucial do processo de cicatrização dos tecidos. O objetivo deste trabalho foi compreender o papel das proteínas da fração soro do látex de H. speciosa na angiogênese. Para isso, esse material foi tratado com proteinase K e as proteínas inativadas. Após a inativação, a atividade angiogênica foi avaliada com o uso do ensaio da membrana corioalantóide de embrião de galinha. Como resultado, foi observado que as proteínas presentes na fração soro são responsáveis pela angiogênese. Como as proteínas mostraram importante papel na angiogênese, foi investigado o teor de proteínas totais presentes na fração soro, bem como sua atividade enzimática. O baixo teor de proteína observado no látex da fração sérica de H. speciosa sugere que esse biomaterial possa ser utilizado para o desenvolvimento de novos medicamentos com resposta hipoalergênica. Apesar do baixo teor de proteína, foi identificada atividade enzimática significativa de pelo menos 3 enzimas na fração sérica do látex: β-1,3 glucanase, β-glucosidase e proteases. Em geral, essas enzimas parecem influenciar o processo de cicatrização, auxiliando no desbridamento, ajudando na remodelação da matriz extracelular e deposição de colágeno, diminuindo as chances de contaminação por microrganismos. Em conclusão, o processo de cicatrização de feridas depende não apenas das células e dos fatores de crescimento disponíveis no tecido, mas também de enzimas do microambiente da matriz extracelular.EDUFU2022-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6109210.14393/BJ-v38n0a2022-61092Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38086Bioscience Journal ; v. 38 (2022): Continuous Publication; e380861981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/61092/34671Brazil; Contemporary Copyright (c) 2022 Patrícia Lima D'Abadia, Amanda Fernandes Costa, Myllena Tolentino Firmino, Samantha Salomão Caramori, Ruy de Souza Lino-Junior, Fabiana Fonseca Zanoelo, Pablo José Gonçalves, Luciane Almeidahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessD'Abadia, PatriciaFernandes Costa, Amanda Firmino, Myllena Tolentino FirminoCaramori, SamanthaRuy de Souza Lino-JuniorZanoelo, Fabiana Fonseca ZanoeloGonçalves, Pablo José GonçalvesAlmeida, Luciane2022-09-30T16:13:42Zoai:ojs.www.seer.ufu.br:article/61092Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-09-30T16:13:42Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv The role of enzymes in the angiogenic activity of Hancornia speciosa latex
O papel das enzimas na atividade angiogênica do látex de Hancornia speciosa
title The role of enzymes in the angiogenic activity of Hancornia speciosa latex
spellingShingle The role of enzymes in the angiogenic activity of Hancornia speciosa latex
D'Abadia, Patricia
Chick chorioallantoic membrane
Enzymes
Proteinase K
Biological Sciences
title_short The role of enzymes in the angiogenic activity of Hancornia speciosa latex
title_full The role of enzymes in the angiogenic activity of Hancornia speciosa latex
title_fullStr The role of enzymes in the angiogenic activity of Hancornia speciosa latex
title_full_unstemmed The role of enzymes in the angiogenic activity of Hancornia speciosa latex
title_sort The role of enzymes in the angiogenic activity of Hancornia speciosa latex
author D'Abadia, Patricia
author_facet D'Abadia, Patricia
Fernandes Costa, Amanda
Firmino, Myllena Tolentino Firmino
Caramori, Samantha
Ruy de Souza Lino-Junior
Zanoelo, Fabiana Fonseca Zanoelo
Gonçalves, Pablo José Gonçalves
Almeida, Luciane
author_role author
author2 Fernandes Costa, Amanda
Firmino, Myllena Tolentino Firmino
Caramori, Samantha
Ruy de Souza Lino-Junior
Zanoelo, Fabiana Fonseca Zanoelo
Gonçalves, Pablo José Gonçalves
Almeida, Luciane
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv D'Abadia, Patricia
Fernandes Costa, Amanda
Firmino, Myllena Tolentino Firmino
Caramori, Samantha
Ruy de Souza Lino-Junior
Zanoelo, Fabiana Fonseca Zanoelo
Gonçalves, Pablo José Gonçalves
Almeida, Luciane
dc.subject.por.fl_str_mv Chick chorioallantoic membrane
Enzymes
Proteinase K
Biological Sciences
topic Chick chorioallantoic membrane
Enzymes
Proteinase K
Biological Sciences
description The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/61092
10.14393/BJ-v38n0a2022-61092
url https://seer.ufu.br/index.php/biosciencejournal/article/view/61092
identifier_str_mv 10.14393/BJ-v38n0a2022-61092
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/61092/34671
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38086
Bioscience Journal ; v. 38 (2022): Continuous Publication; e38086
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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