Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation

Detalhes bibliográficos
Autor(a) principal: Barichello,T.
Data de Publicação: 2007
Outros Autores: Martins,M.R., Reinke,A., Constantino,L.S., Machado,R.A., Valvassori,S.S., Moreira,J.C.F., Quevedo,J., Dal-Pizzol,F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600013
Resumo: Sepsis and its complications are the leading causes of mortality in intensive care units, accounting for 10-50% of deaths. Intensive care unit survivors present long-term cognitive impairment, including alterations in memory, attention, concentration, and/or global loss of cognitive function. In the present study, we investigated behavioral alterations in sepsis-surviving rats. One hundred and ten male Wistar rats (3-4 months, 250-300 g) were submitted to cecal ligation and puncture (CLP), and 44 were submitted to sham operation. Forty-four rats (40%) survived after CLP, and all sham-operated animals survived and were used as control. Twenty animals of each group were used in the object recognition task (10 in short-term memory and 10 in long-term memory), 12 in the plus-maze test and 12 in the forced swimming test. Ten days after surgery, the animals were submitted individually to an object recognition task, plus-maze and forced swimming tests. A significant impairment of short- and long-term recognition memory was observed in the sepsis group (recognition index 0.75 vs 0.55 and 0.74 vs 0.51 for short- and long-term memory, respectively (P < 0.05). In the elevated plus-maze test no difference was observed between groups in any of the parameters assessed. In addition, sepsis survivors presented an increase in immobility time in the forced swimming test (180 vs 233 s, P < 0.05), suggesting the presence of depressive-like symptoms in these animals after recovery from sepsis. The present results demonstrated that rats surviving exposure to CLP, a classical sepsis model, presented recognition memory impairment and depressive-like symptoms but not anxiety-like behavior.
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spelling Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforationSepsis survivorsRecognition memoryPlus-maze testForced swimming testCecal ligation and punctureCognitive impairmentSepsis and its complications are the leading causes of mortality in intensive care units, accounting for 10-50% of deaths. Intensive care unit survivors present long-term cognitive impairment, including alterations in memory, attention, concentration, and/or global loss of cognitive function. In the present study, we investigated behavioral alterations in sepsis-surviving rats. One hundred and ten male Wistar rats (3-4 months, 250-300 g) were submitted to cecal ligation and puncture (CLP), and 44 were submitted to sham operation. Forty-four rats (40%) survived after CLP, and all sham-operated animals survived and were used as control. Twenty animals of each group were used in the object recognition task (10 in short-term memory and 10 in long-term memory), 12 in the plus-maze test and 12 in the forced swimming test. Ten days after surgery, the animals were submitted individually to an object recognition task, plus-maze and forced swimming tests. A significant impairment of short- and long-term recognition memory was observed in the sepsis group (recognition index 0.75 vs 0.55 and 0.74 vs 0.51 for short- and long-term memory, respectively (P < 0.05). In the elevated plus-maze test no difference was observed between groups in any of the parameters assessed. In addition, sepsis survivors presented an increase in immobility time in the forced swimming test (180 vs 233 s, P < 0.05), suggesting the presence of depressive-like symptoms in these animals after recovery from sepsis. The present results demonstrated that rats surviving exposure to CLP, a classical sepsis model, presented recognition memory impairment and depressive-like symptoms but not anxiety-like behavior.Associação Brasileira de Divulgação Científica2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600013Brazilian Journal of Medical and Biological Research v.40 n.6 2007reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2007000600013info:eu-repo/semantics/openAccessBarichello,T.Martins,M.R.Reinke,A.Constantino,L.S.Machado,R.A.Valvassori,S.S.Moreira,J.C.F.Quevedo,J.Dal-Pizzol,F.eng2007-06-18T00:00:00Zoai:scielo:S0100-879X2007000600013Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2007-06-18T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
title Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
spellingShingle Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
Barichello,T.
Sepsis survivors
Recognition memory
Plus-maze test
Forced swimming test
Cecal ligation and puncture
Cognitive impairment
title_short Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
title_full Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
title_fullStr Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
title_full_unstemmed Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
title_sort Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation
author Barichello,T.
author_facet Barichello,T.
Martins,M.R.
Reinke,A.
Constantino,L.S.
Machado,R.A.
Valvassori,S.S.
Moreira,J.C.F.
Quevedo,J.
Dal-Pizzol,F.
author_role author
author2 Martins,M.R.
Reinke,A.
Constantino,L.S.
Machado,R.A.
Valvassori,S.S.
Moreira,J.C.F.
Quevedo,J.
Dal-Pizzol,F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barichello,T.
Martins,M.R.
Reinke,A.
Constantino,L.S.
Machado,R.A.
Valvassori,S.S.
Moreira,J.C.F.
Quevedo,J.
Dal-Pizzol,F.
dc.subject.por.fl_str_mv Sepsis survivors
Recognition memory
Plus-maze test
Forced swimming test
Cecal ligation and puncture
Cognitive impairment
topic Sepsis survivors
Recognition memory
Plus-maze test
Forced swimming test
Cecal ligation and puncture
Cognitive impairment
description Sepsis and its complications are the leading causes of mortality in intensive care units, accounting for 10-50% of deaths. Intensive care unit survivors present long-term cognitive impairment, including alterations in memory, attention, concentration, and/or global loss of cognitive function. In the present study, we investigated behavioral alterations in sepsis-surviving rats. One hundred and ten male Wistar rats (3-4 months, 250-300 g) were submitted to cecal ligation and puncture (CLP), and 44 were submitted to sham operation. Forty-four rats (40%) survived after CLP, and all sham-operated animals survived and were used as control. Twenty animals of each group were used in the object recognition task (10 in short-term memory and 10 in long-term memory), 12 in the plus-maze test and 12 in the forced swimming test. Ten days after surgery, the animals were submitted individually to an object recognition task, plus-maze and forced swimming tests. A significant impairment of short- and long-term recognition memory was observed in the sepsis group (recognition index 0.75 vs 0.55 and 0.74 vs 0.51 for short- and long-term memory, respectively (P < 0.05). In the elevated plus-maze test no difference was observed between groups in any of the parameters assessed. In addition, sepsis survivors presented an increase in immobility time in the forced swimming test (180 vs 233 s, P < 0.05), suggesting the presence of depressive-like symptoms in these animals after recovery from sepsis. The present results demonstrated that rats surviving exposure to CLP, a classical sepsis model, presented recognition memory impairment and depressive-like symptoms but not anxiety-like behavior.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2007000600013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.40 n.6 2007
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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