Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFMA |
Texto Completo: | https://tedebc.ufma.br/jspui/handle/tede/tede/2331 |
Resumo: | Sepsis is defined by the presence of organic dysfunction resulting from infection and generalized inflammation, with high mortality rates, especially among diabetic patients. For this reason, the objective of the present study was to evaluate the effect of the ethanol extract of the flowers of Anacardium occidentale L. in an experimental model of sepsis in diabetic mice. Five-month-old male Swiss mice were used. Diabetes was induced by ad libitum intake of 40% fructose, added to water, for 21 days. On day 14 animals received Streptozotocin (40 mg / kg ip.). Animals with glucose > 200mg / dL were considered diabetic. To determine survival, sepsis by cecal puncture was induced in diabetic animals, which were divided into the following groups (N = 5): Control: received saline; ATB: treated with Imipenem (8mg / kg), subcutaneously (sc) at the time of induction of sepsis and oral EEA: 5mg / kg of Ethanol Extract from Anacardium occidentale, orally, during sepsis. The remaining animals (N = 7 / group), following the same division of the previous groups, were euthanized to evaluate EEA efficacy considering: cell migration; peritoneal inflammatory exudate; cellularity; (H2O2), Nitric Oxide (NO) and cytokine production IL-6, IL-10, MCP-1, INF-y, TNF-α and IL-12. The association with fructose and streptozotocin induced diabetes in Swiss mice. The extract of the flowers of Anacardium occidentale increased the survival of diabetic mice at the time of sepsis but did not prolong it for a long time. On the other hand, it reduced the bacterial infection in the blood, but it was not effective in controlling the bacterial growth in the peritoneum and lung, however, it acted in the balance between inflammatory mediators, increasing the production of hydrogen peroxide and reducing the production of nitric oxide, in addition, it proved to be efficient in controlling exacerbated systemic inflammation in sepsis, by reducing pro-inflammatory cytokines such as IL- 6, TNF-α and the chemokine MCP-1. We conclude that the treatment offered presents promising results regarding the action of Anacardium occidentale on sepsis induced in diabetic mice. |
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GUERRA, Rosane Nassar Meireleshttp://lattes.cnpq.br/2316192786452127001.795.833-44http://lattes.cnpq.br/2751713874701874OLIVEIRA, Aluisio da Silva2018-08-07T15:39:38Z2018-05-08OLIVEIRA, Aluisio da Silva. Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos. 2018. 30 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal do Maranhão, São Luís, 2018.https://tedebc.ufma.br/jspui/handle/tede/tede/2331Sepsis is defined by the presence of organic dysfunction resulting from infection and generalized inflammation, with high mortality rates, especially among diabetic patients. For this reason, the objective of the present study was to evaluate the effect of the ethanol extract of the flowers of Anacardium occidentale L. in an experimental model of sepsis in diabetic mice. Five-month-old male Swiss mice were used. Diabetes was induced by ad libitum intake of 40% fructose, added to water, for 21 days. On day 14 animals received Streptozotocin (40 mg / kg ip.). Animals with glucose > 200mg / dL were considered diabetic. To determine survival, sepsis by cecal puncture was induced in diabetic animals, which were divided into the following groups (N = 5): Control: received saline; ATB: treated with Imipenem (8mg / kg), subcutaneously (sc) at the time of induction of sepsis and oral EEA: 5mg / kg of Ethanol Extract from Anacardium occidentale, orally, during sepsis. The remaining animals (N = 7 / group), following the same division of the previous groups, were euthanized to evaluate EEA efficacy considering: cell migration; peritoneal inflammatory exudate; cellularity; (H2O2), Nitric Oxide (NO) and cytokine production IL-6, IL-10, MCP-1, INF-y, TNF-α and IL-12. The association with fructose and streptozotocin induced diabetes in Swiss mice. The extract of the flowers of Anacardium occidentale increased the survival of diabetic mice at the time of sepsis but did not prolong it for a long time. On the other hand, it reduced the bacterial infection in the blood, but it was not effective in controlling the bacterial growth in the peritoneum and lung, however, it acted in the balance between inflammatory mediators, increasing the production of hydrogen peroxide and reducing the production of nitric oxide, in addition, it proved to be efficient in controlling exacerbated systemic inflammation in sepsis, by reducing pro-inflammatory cytokines such as IL- 6, TNF-α and the chemokine MCP-1. We conclude that the treatment offered presents promising results regarding the action of Anacardium occidentale on sepsis induced in diabetic mice.Sepse é definida pela presença de disfunção orgânica decorrente de infecção e inflamação generalizada, com elevadas taxas de mortalidade, sobretudo entre os pacientes diabéticos. Por esse motivo, o objetivo do presente estudo foi avaliar o efeito do extrato etanólico das flores de Anacardium occidentale L. em modelo experimental de sepse, em camundongos diabéticos. Foram utilizados camundongos Swiss, machos, com cinco meses de idade. A diabetes foi induzida pela ingestão ad libitum de frutose 40%, adicionada à água, durante 21 dias. No 14º dia os animais receberam estreptozotocina (40mg/kg ip.). Foram considerados diabéticos os animais com glicose > 200mg/dL. Para determinar a sobrevida, a sepse por perfuração cecal foi induzida nos animais diabéticos, que foram divididos nos seguintes grupos (N=5): Controle: recebeu salina; ATB: tratado com Imipenem (8mg/kg), via subcutânea (sc) no momento da indução da sepse e EEA oral: 5mg/kg de Extrato Etanólico de Anacardium occidentale, via oral, durante a sepse. Os demais animais (N=7/grupo), seguindo a mesma divisão dos grupos anteriores, foram eutanasiados para avaliar eficácia do EEA considerando: a migração celular; exsudado inflamatório peritoneal; celularidade; efeito antimicrobiano, produção de peróxido de hidrogênio (H2O2), óxido nítrico (NO) e produção das citocinas IL-6, IL-10, MCP-1, IFN-, TNF-α e IL-12. A associação com frutose e estreptozotocina induziu diabetes em camundongos Swiss. O extrato das flores de Anacardium occidentale aumentou a sobrevida de camundongos diabéticos, no momento da sepse, reduziu a infecção bacteriana no sangue e no pulmão, mas não apresentou eficácia em controlar o crescimento bacteriano no peritônio, no entanto, atuou na balanço entre mediadores inflamatório, aumentando a produção de peróxido de hidrogênio e reduzindo a produção de óxido nítrico, além disso, mostrou-se eficiente em controlar a inflamação sistêmica exacerbada na sepse, por reduzir citocinas pro-inflamatórias como IL-6, TNF-α e a quimiocinas MCP-1. Concluímos que o tratamento oferecido apresenta resultados promissores quanto a ação de Anacardium occidentale na sepse induzida em camundongos diabéticos.Submitted by Rosivalda Pereira (mrs.pereira@ufma.br) on 2018-08-07T15:39:38Z No. of bitstreams: 1 AluisioOliveira.pdf: 672502 bytes, checksum: 527931b03322209d958d071ff4707021 (MD5)Made available in DSpace on 2018-08-07T15:39:38Z (GMT). No. of bitstreams: 1 AluisioOliveira.pdf: 672502 bytes, checksum: 527931b03322209d958d071ff4707021 (MD5) Previous issue date: 2018-05-08FAPEMACNPqapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBSUFMABrasilDEPARTAMENTO DE MEDICINA II/CCBSAnacardium occidentaleSepseDiabetesCitocinasPerfuração cecalAntiinflamatórioSepsisCytokinesCecal ligation and punctureCiências da SaúdeEfeitos de Anacardium occidentale L. na sepse de camundongos diabéticosEffects of Anacardium occidentale L. on sepsis in diabetic miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALAluisioOliveira.pdfAluisioOliveira.pdfapplication/pdf672502http://tedebc.ufma.br:8080/bitstream/tede/2331/2/AluisioOliveira.pdf527931b03322209d958d071ff4707021MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/2331/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/23312018-08-07 12:39:38.492oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312018-08-07T15:39:38Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
dc.title.por.fl_str_mv |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
dc.title.alternative.eng.fl_str_mv |
Effects of Anacardium occidentale L. on sepsis in diabetic mice |
title |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
spellingShingle |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos OLIVEIRA, Aluisio da Silva Anacardium occidentale Sepse Diabetes Citocinas Perfuração cecal Antiinflamatório Sepsis Cytokines Cecal ligation and puncture Ciências da Saúde |
title_short |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
title_full |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
title_fullStr |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
title_full_unstemmed |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
title_sort |
Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos |
author |
OLIVEIRA, Aluisio da Silva |
author_facet |
OLIVEIRA, Aluisio da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
GUERRA, Rosane Nassar Meireles |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2316192786452127 |
dc.contributor.authorID.fl_str_mv |
001.795.833-44 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2751713874701874 |
dc.contributor.author.fl_str_mv |
OLIVEIRA, Aluisio da Silva |
contributor_str_mv |
GUERRA, Rosane Nassar Meireles |
dc.subject.por.fl_str_mv |
Anacardium occidentale Sepse Diabetes Citocinas Perfuração cecal Antiinflamatório |
topic |
Anacardium occidentale Sepse Diabetes Citocinas Perfuração cecal Antiinflamatório Sepsis Cytokines Cecal ligation and puncture Ciências da Saúde |
dc.subject.eng.fl_str_mv |
Sepsis Cytokines Cecal ligation and puncture |
dc.subject.cnpq.fl_str_mv |
Ciências da Saúde |
description |
Sepsis is defined by the presence of organic dysfunction resulting from infection and generalized inflammation, with high mortality rates, especially among diabetic patients. For this reason, the objective of the present study was to evaluate the effect of the ethanol extract of the flowers of Anacardium occidentale L. in an experimental model of sepsis in diabetic mice. Five-month-old male Swiss mice were used. Diabetes was induced by ad libitum intake of 40% fructose, added to water, for 21 days. On day 14 animals received Streptozotocin (40 mg / kg ip.). Animals with glucose > 200mg / dL were considered diabetic. To determine survival, sepsis by cecal puncture was induced in diabetic animals, which were divided into the following groups (N = 5): Control: received saline; ATB: treated with Imipenem (8mg / kg), subcutaneously (sc) at the time of induction of sepsis and oral EEA: 5mg / kg of Ethanol Extract from Anacardium occidentale, orally, during sepsis. The remaining animals (N = 7 / group), following the same division of the previous groups, were euthanized to evaluate EEA efficacy considering: cell migration; peritoneal inflammatory exudate; cellularity; (H2O2), Nitric Oxide (NO) and cytokine production IL-6, IL-10, MCP-1, INF-y, TNF-α and IL-12. The association with fructose and streptozotocin induced diabetes in Swiss mice. The extract of the flowers of Anacardium occidentale increased the survival of diabetic mice at the time of sepsis but did not prolong it for a long time. On the other hand, it reduced the bacterial infection in the blood, but it was not effective in controlling the bacterial growth in the peritoneum and lung, however, it acted in the balance between inflammatory mediators, increasing the production of hydrogen peroxide and reducing the production of nitric oxide, in addition, it proved to be efficient in controlling exacerbated systemic inflammation in sepsis, by reducing pro-inflammatory cytokines such as IL- 6, TNF-α and the chemokine MCP-1. We conclude that the treatment offered presents promising results regarding the action of Anacardium occidentale on sepsis induced in diabetic mice. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-08-07T15:39:38Z |
dc.date.issued.fl_str_mv |
2018-05-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Aluisio da Silva. Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos. 2018. 30 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal do Maranhão, São Luís, 2018. |
dc.identifier.uri.fl_str_mv |
https://tedebc.ufma.br/jspui/handle/tede/tede/2331 |
identifier_str_mv |
OLIVEIRA, Aluisio da Silva. Efeitos de Anacardium occidentale L. na sepse de camundongos diabéticos. 2018. 30 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal do Maranhão, São Luís, 2018. |
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por |
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Universidade Federal do Maranhão |
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UFMA |
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Brasil |
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Universidade Federal do Maranhão |
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