Pressure-induced dissociation of casein micelles: size distribution and effect of temperature

Detalhes bibliográficos
Autor(a) principal: Gebhardt,R.
Data de Publicação: 2005
Outros Autores: Doster,W., Kulozik,U.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800008
Resumo: Pressure-induced dissociation of a turbid solution of casein micelles was studied in situ in static and dynamic light scattering experiments. We show that at high pressure casein micelles decompose into small fragments comparable in size to casein monomers. At intermediate pressure we observe particles measuring 15 to 20 nm in diameter. The stability against pressure dissociation increased with temperature, suggesting enhanced hydrophobic contacts. The pressure transition curves are biphasic, compatible with a temperature (but not pressure)-dependent conformational equilibrium of two micelle species. Our thermodynamic model predicts an increase in structural entropy with temperature.
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spelling Pressure-induced dissociation of casein micelles: size distribution and effect of temperatureCasein micellesHigh-pressure light scatteringPressure dissociationPressure-induced dissociation of a turbid solution of casein micelles was studied in situ in static and dynamic light scattering experiments. We show that at high pressure casein micelles decompose into small fragments comparable in size to casein monomers. At intermediate pressure we observe particles measuring 15 to 20 nm in diameter. The stability against pressure dissociation increased with temperature, suggesting enhanced hydrophobic contacts. The pressure transition curves are biphasic, compatible with a temperature (but not pressure)-dependent conformational equilibrium of two micelle species. Our thermodynamic model predicts an increase in structural entropy with temperature.Associação Brasileira de Divulgação Científica2005-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800008Brazilian Journal of Medical and Biological Research v.38 n.8 2005reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2005000800008info:eu-repo/semantics/openAccessGebhardt,R.Doster,W.Kulozik,U.eng2005-07-30T00:00:00Zoai:scielo:S0100-879X2005000800008Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2005-07-30T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
title Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
spellingShingle Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
Gebhardt,R.
Casein micelles
High-pressure light scattering
Pressure dissociation
title_short Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
title_full Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
title_fullStr Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
title_full_unstemmed Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
title_sort Pressure-induced dissociation of casein micelles: size distribution and effect of temperature
author Gebhardt,R.
author_facet Gebhardt,R.
Doster,W.
Kulozik,U.
author_role author
author2 Doster,W.
Kulozik,U.
author2_role author
author
dc.contributor.author.fl_str_mv Gebhardt,R.
Doster,W.
Kulozik,U.
dc.subject.por.fl_str_mv Casein micelles
High-pressure light scattering
Pressure dissociation
topic Casein micelles
High-pressure light scattering
Pressure dissociation
description Pressure-induced dissociation of a turbid solution of casein micelles was studied in situ in static and dynamic light scattering experiments. We show that at high pressure casein micelles decompose into small fragments comparable in size to casein monomers. At intermediate pressure we observe particles measuring 15 to 20 nm in diameter. The stability against pressure dissociation increased with temperature, suggesting enhanced hydrophobic contacts. The pressure transition curves are biphasic, compatible with a temperature (but not pressure)-dependent conformational equilibrium of two micelle species. Our thermodynamic model predicts an increase in structural entropy with temperature.
publishDate 2005
dc.date.none.fl_str_mv 2005-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2005000800008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.38 n.8 2005
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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