Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600012 |
Resumo: | It has been shown that angiotensin-(1-7) (Ang-(1-7)) infusion potentiates the bradykinin (BK)-induced hypotensive response in conscious rats. The present study was conducted to identify Ang-(1-7)-BK interactions in the isolated rat heart perfused according to the Langendorff technique. Hearts were excised and perfused through the aortic stump under a constant flow with Krebs-Ringer solution and the changes in perfusion pressure and heart contractile force were recorded. Bolus injections of BK (2.5, 5, 10 and 20 ng) produced a dose-dependent hypotensive effect. Ang-(1-7) added to the perfusion solution (2 ng/ml) did not change the perfusion pressure or the contractile force but doubled the hypotensive effect of the lower doses of BK. The BK-potentiating Ang-(1-7) activity was blocked by pretreatment with indomethacin (5 mg/kg, ip) or L-NAME (30 mg/kg, ip). The Ang-(1-7) antagonist A-779 (50 ng/ml in Krebs-Ringer) completely blocked the effect of Ang-(1-7) on BK-induced vasodilation. These data suggest that the potentiation of the BK-induced vasodilation by Ang-(1-7) can be attributed to the release of nitric oxide and vasodilator prostaglandins through an Ang-(1-7) receptor-mediated mechanism. |
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Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heartangiotensin-(1-7)bradykinincoronary arteryrat heartL-NAMEprostaglandinsbradykinin potentiationIt has been shown that angiotensin-(1-7) (Ang-(1-7)) infusion potentiates the bradykinin (BK)-induced hypotensive response in conscious rats. The present study was conducted to identify Ang-(1-7)-BK interactions in the isolated rat heart perfused according to the Langendorff technique. Hearts were excised and perfused through the aortic stump under a constant flow with Krebs-Ringer solution and the changes in perfusion pressure and heart contractile force were recorded. Bolus injections of BK (2.5, 5, 10 and 20 ng) produced a dose-dependent hypotensive effect. Ang-(1-7) added to the perfusion solution (2 ng/ml) did not change the perfusion pressure or the contractile force but doubled the hypotensive effect of the lower doses of BK. The BK-potentiating Ang-(1-7) activity was blocked by pretreatment with indomethacin (5 mg/kg, ip) or L-NAME (30 mg/kg, ip). The Ang-(1-7) antagonist A-779 (50 ng/ml in Krebs-Ringer) completely blocked the effect of Ang-(1-7) on BK-induced vasodilation. These data suggest that the potentiation of the BK-induced vasodilation by Ang-(1-7) can be attributed to the release of nitric oxide and vasodilator prostaglandins through an Ang-(1-7) receptor-mediated mechanism.Associação Brasileira de Divulgação Científica2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600012Brazilian Journal of Medical and Biological Research v.33 n.6 2000reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2000000600012info:eu-repo/semantics/openAccessAlmeida,A.P.Frábregas,B.C.Madureira,M.M.Santos,R.J.S.Campagnole-Santos,M.J.Santos,R.A.S.eng2000-05-30T00:00:00Zoai:scielo:S0100-879X2000000600012Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2000-05-30T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
title |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
spellingShingle |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart Almeida,A.P. angiotensin-(1-7) bradykinin coronary artery rat heart L-NAME prostaglandins bradykinin potentiation |
title_short |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
title_full |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
title_fullStr |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
title_full_unstemmed |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
title_sort |
Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart |
author |
Almeida,A.P. |
author_facet |
Almeida,A.P. Frábregas,B.C. Madureira,M.M. Santos,R.J.S. Campagnole-Santos,M.J. Santos,R.A.S. |
author_role |
author |
author2 |
Frábregas,B.C. Madureira,M.M. Santos,R.J.S. Campagnole-Santos,M.J. Santos,R.A.S. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Almeida,A.P. Frábregas,B.C. Madureira,M.M. Santos,R.J.S. Campagnole-Santos,M.J. Santos,R.A.S. |
dc.subject.por.fl_str_mv |
angiotensin-(1-7) bradykinin coronary artery rat heart L-NAME prostaglandins bradykinin potentiation |
topic |
angiotensin-(1-7) bradykinin coronary artery rat heart L-NAME prostaglandins bradykinin potentiation |
description |
It has been shown that angiotensin-(1-7) (Ang-(1-7)) infusion potentiates the bradykinin (BK)-induced hypotensive response in conscious rats. The present study was conducted to identify Ang-(1-7)-BK interactions in the isolated rat heart perfused according to the Langendorff technique. Hearts were excised and perfused through the aortic stump under a constant flow with Krebs-Ringer solution and the changes in perfusion pressure and heart contractile force were recorded. Bolus injections of BK (2.5, 5, 10 and 20 ng) produced a dose-dependent hypotensive effect. Ang-(1-7) added to the perfusion solution (2 ng/ml) did not change the perfusion pressure or the contractile force but doubled the hypotensive effect of the lower doses of BK. The BK-potentiating Ang-(1-7) activity was blocked by pretreatment with indomethacin (5 mg/kg, ip) or L-NAME (30 mg/kg, ip). The Ang-(1-7) antagonist A-779 (50 ng/ml in Krebs-Ringer) completely blocked the effect of Ang-(1-7) on BK-induced vasodilation. These data suggest that the potentiation of the BK-induced vasodilation by Ang-(1-7) can be attributed to the release of nitric oxide and vasodilator prostaglandins through an Ang-(1-7) receptor-mediated mechanism. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2000000600012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.33 n.6 2000 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302930487345152 |