Human group C rotavirus in children with diarrhea in the Federal District, Brazil

Detalhes bibliográficos
Autor(a) principal: Teixeira,J.M.S.
Data de Publicação: 1998
Outros Autores: Camara,G.N.N.L., Pimentel,P.F.V., Ferreira,M.N.R., Ferreira,M.S.R., Alfieri,A.A., Gentsch,J.R., Leite,J.P.G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005
Resumo: Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an HIV-seropositive adult male with diarrhea of sudden onset. All samples were analyzed by enzyme immunoassay for group A rotavirus and adenovirus (EIARA) and by polyacrylamide gel electrophoresis (PAGE). One hundred and seven (26%) were positive for group A rotavirus. Four samples from children and the sample from the HIV-seropositive patient, although negative by EIARA, showed a group C rotavirus profile by PAGE and were positive for rotavirus by electron microscopy. Using specific VP6 and VP7 primers for group C rotavirus, a reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and products were detected by agarose gel electrophoresis and ethidium bromide staining. These products were confirmed to be specific for group C rotavirus by using digoxigenin-oligonucleotide probes, Southern hybridization and chemiluminescent detection. The five positive group C rotavirus samples were detected in August (3 samples) and September (2 samples). To the best of our knowledge, this is the first report of group C rotavirus detected in the Federal District, Brazil and in an HIV-seropositive patient with acute gastroenteritis.
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spelling Human group C rotavirus in children with diarrhea in the Federal District, Brazilgroup C rotavirusdiarrheaHIVRT-PCRprobe hybridizationGroup C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an HIV-seropositive adult male with diarrhea of sudden onset. All samples were analyzed by enzyme immunoassay for group A rotavirus and adenovirus (EIARA) and by polyacrylamide gel electrophoresis (PAGE). One hundred and seven (26%) were positive for group A rotavirus. Four samples from children and the sample from the HIV-seropositive patient, although negative by EIARA, showed a group C rotavirus profile by PAGE and were positive for rotavirus by electron microscopy. Using specific VP6 and VP7 primers for group C rotavirus, a reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and products were detected by agarose gel electrophoresis and ethidium bromide staining. These products were confirmed to be specific for group C rotavirus by using digoxigenin-oligonucleotide probes, Southern hybridization and chemiluminescent detection. The five positive group C rotavirus samples were detected in August (3 samples) and September (2 samples). To the best of our knowledge, this is the first report of group C rotavirus detected in the Federal District, Brazil and in an HIV-seropositive patient with acute gastroenteritis.Associação Brasileira de Divulgação Científica1998-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005Brazilian Journal of Medical and Biological Research v.31 n.11 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998001100005info:eu-repo/semantics/openAccessTeixeira,J.M.S.Camara,G.N.N.L.Pimentel,P.F.V.Ferreira,M.N.R.Ferreira,M.S.R.Alfieri,A.A.Gentsch,J.R.Leite,J.P.G.eng1998-10-20T00:00:00Zoai:scielo:S0100-879X1998001100005Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Human group C rotavirus in children with diarrhea in the Federal District, Brazil
title Human group C rotavirus in children with diarrhea in the Federal District, Brazil
spellingShingle Human group C rotavirus in children with diarrhea in the Federal District, Brazil
Teixeira,J.M.S.
group C rotavirus
diarrhea
HIV
RT-PCR
probe hybridization
title_short Human group C rotavirus in children with diarrhea in the Federal District, Brazil
title_full Human group C rotavirus in children with diarrhea in the Federal District, Brazil
title_fullStr Human group C rotavirus in children with diarrhea in the Federal District, Brazil
title_full_unstemmed Human group C rotavirus in children with diarrhea in the Federal District, Brazil
title_sort Human group C rotavirus in children with diarrhea in the Federal District, Brazil
author Teixeira,J.M.S.
author_facet Teixeira,J.M.S.
Camara,G.N.N.L.
Pimentel,P.F.V.
Ferreira,M.N.R.
Ferreira,M.S.R.
Alfieri,A.A.
Gentsch,J.R.
Leite,J.P.G.
author_role author
author2 Camara,G.N.N.L.
Pimentel,P.F.V.
Ferreira,M.N.R.
Ferreira,M.S.R.
Alfieri,A.A.
Gentsch,J.R.
Leite,J.P.G.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira,J.M.S.
Camara,G.N.N.L.
Pimentel,P.F.V.
Ferreira,M.N.R.
Ferreira,M.S.R.
Alfieri,A.A.
Gentsch,J.R.
Leite,J.P.G.
dc.subject.por.fl_str_mv group C rotavirus
diarrhea
HIV
RT-PCR
probe hybridization
topic group C rotavirus
diarrhea
HIV
RT-PCR
probe hybridization
description Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an HIV-seropositive adult male with diarrhea of sudden onset. All samples were analyzed by enzyme immunoassay for group A rotavirus and adenovirus (EIARA) and by polyacrylamide gel electrophoresis (PAGE). One hundred and seven (26%) were positive for group A rotavirus. Four samples from children and the sample from the HIV-seropositive patient, although negative by EIARA, showed a group C rotavirus profile by PAGE and were positive for rotavirus by electron microscopy. Using specific VP6 and VP7 primers for group C rotavirus, a reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and products were detected by agarose gel electrophoresis and ethidium bromide staining. These products were confirmed to be specific for group C rotavirus by using digoxigenin-oligonucleotide probes, Southern hybridization and chemiluminescent detection. The five positive group C rotavirus samples were detected in August (3 samples) and September (2 samples). To the best of our knowledge, this is the first report of group C rotavirus detected in the Federal District, Brazil and in an HIV-seropositive patient with acute gastroenteritis.
publishDate 1998
dc.date.none.fl_str_mv 1998-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S0100-879X1998001100005
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dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.31 n.11 1998
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
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reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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