Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases

Detalhes bibliográficos
Autor(a) principal: Sukoyan,M.A.
Data de Publicação: 2002
Outros Autores: Kerkis,A.Y., Mello,M.R.B., Kerkis,I.E., Visintin,J.A., Pereira,L.V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004
Resumo: Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase activity and their capacity to form complex embryoid bodies with rhythmically contracting cardiomyocytes. Two lines, USP-1 and USP-3, with the best in vitro characteristics of pluripotency were used in chimera-generating experiments. The capacity to contribute to the germ line was demonstrated by the USP-1 cell line. This cell line is currently being used to generate mouse models of human diseases.
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spelling Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseasesHuman genetic diseasesMurine embryonic stem cellsMouse modelTransgenic mouseEmbryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase activity and their capacity to form complex embryoid bodies with rhythmically contracting cardiomyocytes. Two lines, USP-1 and USP-3, with the best in vitro characteristics of pluripotency were used in chimera-generating experiments. The capacity to contribute to the germ line was demonstrated by the USP-1 cell line. This cell line is currently being used to generate mouse models of human diseases.Associação Brasileira de Divulgação Científica2002-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004Brazilian Journal of Medical and Biological Research v.35 n.5 2002reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2002000500004info:eu-repo/semantics/openAccessSukoyan,M.A.Kerkis,A.Y.Mello,M.R.B.Kerkis,I.E.Visintin,J.A.Pereira,L.V.eng2002-05-06T00:00:00Zoai:scielo:S0100-879X2002000500004Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2002-05-06T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
title Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
spellingShingle Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
Sukoyan,M.A.
Human genetic diseases
Murine embryonic stem cells
Mouse model
Transgenic mouse
title_short Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
title_full Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
title_fullStr Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
title_full_unstemmed Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
title_sort Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
author Sukoyan,M.A.
author_facet Sukoyan,M.A.
Kerkis,A.Y.
Mello,M.R.B.
Kerkis,I.E.
Visintin,J.A.
Pereira,L.V.
author_role author
author2 Kerkis,A.Y.
Mello,M.R.B.
Kerkis,I.E.
Visintin,J.A.
Pereira,L.V.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sukoyan,M.A.
Kerkis,A.Y.
Mello,M.R.B.
Kerkis,I.E.
Visintin,J.A.
Pereira,L.V.
dc.subject.por.fl_str_mv Human genetic diseases
Murine embryonic stem cells
Mouse model
Transgenic mouse
topic Human genetic diseases
Murine embryonic stem cells
Mouse model
Transgenic mouse
description Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase activity and their capacity to form complex embryoid bodies with rhythmically contracting cardiomyocytes. Two lines, USP-1 and USP-3, with the best in vitro characteristics of pluripotency were used in chimera-generating experiments. The capacity to contribute to the germ line was demonstrated by the USP-1 cell line. This cell line is currently being used to generate mouse models of human diseases.
publishDate 2002
dc.date.none.fl_str_mv 2002-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2002000500004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.35 n.5 2002
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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